Boron induces osteogenesis by stimulating NaBC1 in cooperation with BMPR1A

Rico, Patricia; Rodrigo‐Navarro, Aleixandre; Pérez, Laura Sánchez; Salmerón-Sánchez, Manuel

doi:10.1101/2020.03.17.995001

Cited by 2 publications

(2 citation statements)

References 61 publications

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“…The datasets generated during and/or analyzed during this study are available in the University of Glasgow Repository, http://researchdata.gla.ac.uk/ 65 .…”

Section: Data Availabilitymentioning

confidence: 99%

Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

et al. 2020

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The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/α5β1/αvβ3 co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors.

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“…The datasets generated during and/or analyzed during this study are available in the University of Glasgow Repository, http://researchdata.gla.ac.uk/ 65 .…”

Section: Data Availabilitymentioning

confidence: 99%

Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

et al. 2020

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“…17 Furthermore, there is growing interest in boron homeostasis, more precisely, in sodium boron cotransporter 1 (NaBC1), which controls boron homeostasis and takes part in myogenic differentiation. 18 Boron presence also enhances the availability of tumour necrosis factor -α (TNF-α) in fibroblasts, which in turn induces angiogenesis, augments gene expression responsible for inflammatory mediators, and represses the transcription of collagen gene. 19,20 Among precursors of boron, boric acid stands out as a long used medium for deep wound healing as it possesses antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

Boric Acid Modified Chitosan Scaffolds Chemically Crosslinked by Genipin

Dornjak¹,

Ostojić²,

Klaser³

et al. 2022

Kemija U Industriji

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Chitosan scaffolds are an effective biologically active material with versatile application in chemistry and medicine. Chitosan is a linear polysaccharide, a derivative of chitin, with great biocompatibility due to the possession of functional groups such as −OH and −NH 2 , which allow for biodegradability and antibacterial function. Chitosan has a polycation nature allowing complex formation with metal ions and many biomolecules such as DNA, proteins and lipids, while its specific structure and functional groups are responsible for antibacterial, hemostatic, and analgesic properties. To improve its angiogenic and antimicrobial potential, chitosan can be modified by boron (borate ions). The aim of this work was to prepare boric acid modified chitosan scaffolds, using boric acid as a boron precursor, as potential bioactive scaffolds for tissue regeneration. Borate ions tend to form complexes with hydroxyl groups, however, such physical interactions between boron and chitosan functional groups result in poor encapsulation efficiency. To ensure higher boron incorporation, chitosan scaffolds were cross-linked by genipin, a cross linker with lower cytotoxicity in contrast to glutaraldehyde commonly used to prepare stable chitosan-based materials. The degree of deacetylation (DD) and concentration of chitosan solution as well as the concentration of a solvent are important parameters that affect the crosslinking process. Moreover, the addition of boric acid could interfere with the crosslinking process by occupying chitosan functional groups. Here, chitosan scaffolds were modified with different concentrations of boric acid, while the concentrations of chitosan solution (1.2 w/v), genipin (2 % w/w), and acetic acid (0.5 % v/v) were kept constant. Obtained scaffolds were characterised by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), differential scanning calorimetry (DSC), Fourier transformation infrared spectroscopy (FTIR), while cytotoxicity was evaluated as a function of materials concentration and exposure time. The results indicated successful incorporation of boron into crosslinked chitosan scaffolds with highly porous structure and low cytotoxicity.

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Boron induces osteogenesis by stimulating NaBC1 in cooperation with BMPR1A

Cited by 2 publications

References 61 publications

Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

Boric Acid Modified Chitosan Scaffolds Chemically Crosslinked by Genipin

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