Boron reduces cell wall aluminum content in rice (Oryza sativa) roots by decreasing H2O2 accumulation

Zhu, Chun Quan; Cao, Xiaochuang; Hu, Wengang; Hu, An Yong; Abliz, Buhailiqem; Bai, Zhi Gang; Huang, Jie; Liang, Qing; Hussain, Sajid; Li, Ye Feng; Wang, Li Ping; Jin, Qian; Zhang, Jun Hua

doi:10.1016/j.plaphy.2019.02.022

Cited by 49 publications

(26 citation statements)

References 73 publications

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“…7b), accompanied by lesser cytoplasmic Al ration and higher vacuole Al ratio of root tips after B10 and B50 addition (Fig. S5), and yielded similar results in rice that B application could increase the ALS1 expression (Zhu et al ., 2019), suggesting that B could provoke the transfer of Al from the cytoplasm to the vacuoles, which showing low Al toxicity to seedlings. In addition, the expression of OsSTAR1 and OsSTAR2 , plays an important role in rice Al tolerance (Zhu et al ., 2018; Zhu et al ., 2019), and functions as a transporter of the ATP binding cassette, witch transports UDP-glucose from the cytoplasm to CW, thus reducing the deposition of Al in CW (Huang et al ., 2009).…”

Section: Discussionsupporting

confidence: 64%

“…Boron is indispensable for plant growth and in the mitigation of Al toxicity (Zhu et al ., 2019). In the previous study, we have explored mechanisms by which B could regulate Al stress in the rape seedlings (Yan et al , 2018b) and citrus (Yan et al ., 2018a; Riaz et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…These include shielding the CW of Al-binding site (Yan et al , 2018a), reducing the transfer of Al from the external environment to cells and detoxifying Al inside the apoplast (Zhu et al ., 2018), and compartmentalizes Al into the vacuoles (Xia et al ., 2010). Therefore, enhancing the exclusion of Al from root CW is the pivotal role of plants to resist Al toxicity (Zhu et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, the mitigation mechanisms of B to Al toxicity in plants has been studied. Zhu et al ., (2019) pointed out that B alleviates Al toxicity by altering pectin content and its properties. Moreover, B could enhance Al transfer from the cytoplasm to vacuoles by regulating Al-related transporters in rice root (Zhu et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Zhu et al ., (2019) pointed out that B alleviates Al toxicity by altering pectin content and its properties. Moreover, B could enhance Al transfer from the cytoplasm to vacuoles by regulating Al-related transporters in rice root (Zhu et al ., 2019). However, the mechanisms of B reducing Al toxicity in different plants may be distinct and complex.…”

Section: Introductionmentioning

confidence: 99%

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Boron alters carboxyl group binding capacity and Al transport pathway to relieve Al toxicity

Riaz

Liu

et al. 2019

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Boron (B) is indispensable for plant growth and has been reported in the mitigation of aluminum (Al) toxicity in different plants. This study unraveled the efficacy of B in reducing the toxicity of Al to trifoliate orange seedlings in a hydroponic experiment. In the current study, B supply had a positive effect on root length and plant growth-related parameters, and attenuated Al-induced inhibition of plasma membrane H+-ATPase activity. The results of XPS and SEM-EDS revealed that B reduces the Al accumulation in root cell wall (CW), especially acts on pectin fractions (alkali-soluble pectin), accompanied by suppressing the pectin synthesis, inhibiting pectin methylesterase (PME) activity and PME expression. Furthermore, B application inhibits NRAT1 expression while increases ALS1 expression, which are responsible for restraining Al transport from external cells to the cytoplasm and accelerating Al divert to vacuoles, and the results can be further demonstrated by TEM-EDS analysis. Taken together, our results indicated that B mainly promotes the efflux of H+ by regulating the plasma membrane H+-ATPase activity, futhur reduce the demethylation of pectin to weaken Al binding ability to carboxyl. More importantly, B alleviated some of the toxic effects of Al by decreasing the deposition of Al in cytoplasm and compartmentalizes Al into vacuoles.One-sentence summaryBoron can reduce the binding amount of carboxyl group to Al in pectin, decreasing the deposition of Al in cytoplasm and compartmentalizes Al into vacuoles, thereby reduce the toxicity of Al to plants..

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