Borrelia miyamotoi strain LB-2001 retains plasmids and infectious phenotype throughout continuous culture passages as evaluated by multiplex PCR

Gilmore, Robert D.; Mikula, Sierra; Harris, Emma K.; Gundy, Taylor J. Van; Goodrich, Irina; Brandt, Kevin S.

doi:10.1016/j.ttbdis.2020.101587

Cited by 6 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(i) Assessment of B. miyamotoi LB-2001 acquisition and transmission by I. scapularis on CD-1 mice. Our lab has previously shown that CD-1 mice that are needle inoculated with LB-2001 elicit a robust humoral response against B. miyamotoi antigens and that spirochetes could occasionally be observed in blood smears and detected in organs via PCR ( 51 , 52 ). Based on our findings and on those of other studies conducted on B. miyamotoi and STRF spirochetes, we attempted to generate infected ticks using CD-1 mice inoculated with LB-2001 ( 40 , 41 , 48 , 51 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Both isolates have the standard chromosome, with plasmid variation: LB-2001 has 12 plasmids, 8 linear and 4 circular, and CT13-2396 has 14 plasmids, 9 linear and 5 circular. Upon further investigation, the differences in the plasmid contents were found to be the result of alternative rearrangements [142]. This revealed that B. miyamotoi has the capability for genetic rearrangement among isolates of the same geographic lineage [143].…”

Section: Phylogenetics Genomics and Geneticsmentioning

confidence: 94%

Relapsing Review: A Research Update on <em>Borrelia miyamotoi</em>

Cleveland¹,

Anderson²,

Brissette³

2022

Preprint

View full text Add to dashboard Cite

Borrelia miyamotoi is an emerging tick-borne pathogen in the Northern hemisphere and is the causative agent of Borrelia miyamotoi disease (BMD). B. miyamotoi is vectored by the same hard-bodied ticks as Lyme disease Borrelia, yet phylogenetically groups with relapsing fever Borrelia, and thus has been uniquely labeled a hard tick-borne relapsing fever Borrelia. Burgeoning research has uncovered new aspects of B. miyamotoi in human patients, nature, and the lab. Of particular interest are novel findings on disease pathology, prevalence, diagnostic methods, ecological maintenance, transmission, and genetic characteristics. Herein we review recent literature on B. miyamotoi, discuss how findings adapt to current Borrelia doctrines, and briefly consider what remains unknown about B. miyamotoi.

show abstract

“…Both isolates have the standard chromosome, with plasmid variation: LB-2001 has 12 plasmids, 8 linear and 4 circular, and CT13-2396 has 14 plasmids, 9 linear and 5 circular. Upon further investigation, the differences in the plasmid contents were found to be the result of alternative rearrangements [ 153 ]. This revealed that B. miyamotoi has the capability for genetic rearrangement among isolates of the same geographic lineage [ 154 ].…”

Section: Laboratory Studies On B Miyamotoimentioning

confidence: 99%

Borrelia miyamotoi: A Comprehensive Review

2023

View full text Add to dashboard Cite

Borrelia miyamotoi is an emerging tick-borne pathogen in the Northern Hemisphere and is the causative agent of Borrelia miyamotoi disease (BMD). Borrelia miyamotoi is vectored by the same hard-bodied ticks as Lyme disease Borrelia, yet phylogenetically groups with relapsing fever Borrelia, and thus, has been uniquely labeled a hard tick-borne relapsing fever Borrelia. Burgeoning research has uncovered new aspects of B. miyamotoi in human patients, nature, and the lab. Of particular interest are novel findings on disease pathology, prevalence, diagnostic methods, ecological maintenance, transmission, and genetic characteristics. Herein, we review recent literature on B. miyamotoi, discuss how findings adapt to current Borrelia doctrines, and briefly consider what remains unknown about B. miyamotoi.

show abstract

Borrelia miyamotoi strain LB-2001 retains plasmids and infectious phenotype throughout continuous culture passages as evaluated by multiplex PCR

Cited by 6 publications

References 25 publications

Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

Evaluation of Immunocompetent Mouse Models for Borrelia miyamotoi Infection

Relapsing Review: A Research Update on <em>Borrelia miyamotoi</em>

Borrelia miyamotoi: A Comprehensive Review

Contact Info

Product

Resources

About