Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)

Scola, Bernard La; Mallet, M N; Grimont, Patrick A. D.; Raoult, Didier

doi:10.1099/ijs.0.02127-0

Cited by 81 publications

(36 citation statements)

References 17 publications

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“…Three additional species, B. eneae, B. vestrisii, and B. massiliensis (154), were recovered by amoebal coculture from hospital water supplies (150), demonstrating that some representatives of that genus are ARB commonly present in water. More importantly, they were associated with severe nosocomial pneumonia in ventilated intensive care unit patients (152).…”

Section: Bradyrhizobiaceaementioning

confidence: 99%

Microorganisms Resistant to Free-Living Amoebae

2004

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Free-living amoebae feed on bacteria, fungi, and algae. However, some microorganisms have evolved to become resistant to these protists. These amoeba-resistant microorganisms include established pathogens, such as Cryptococcus neoformans, Legionella spp., Chlamydophila pneumoniae, Mycobacterium avium, Listeria monocytogenes, Pseudomonas aeruginosa, and Francisella tularensis, and emerging pathogens, such as Bosea spp., Simkania negevensis, Parachlamydia acanthamoebae, and Legionella-like amoebal pathogens. Some of these amoeba-resistant bacteria (ARB) are lytic for their amoebal host, while others are considered endosymbionts, since a stable host-parasite ratio is maintained. Free-living amoebae represent an important reservoir of ARB and may, while encysted, protect the internalized bacteria from chlorine and other biocides. Free-living amoebae may act as a Trojan horse, bringing hidden ARB within the human “Troy,” and may produce vesicles filled with ARB, increasing their transmission potential. Free-living amoebae may also play a role in the selection of virulence traits and in adaptation to survival in macrophages. Thus, intra-amoebal growth was found to enhance virulence, and similar mechanisms seem to be implicated in the survival of ARB in response to both amoebae and macrophages. Moreover, free-living amoebae represent a useful tool for the culture of some intracellular bacteria and new bacterial species that might be potential emerging pathogens

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Section: Bradyrhizobiaceaementioning

confidence: 99%

Microorganisms Resistant to Free-Living Amoebae

2004

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“…In our laboratory, the great qualities of several "cultivators," technicians, or researchers were identified and recognized in the names of bacterial species to honor these qualities (i.e., Rickettsia raoultii, Legionella drancourtii, Mycobacterium barrassiae, Afipia birgiae, Bosea vestrisii, and Bosea eneae, etc. [251,384,392,396,397]), as the names of other species acknowledge several "cultivators," such as Finegoldia magna, Murdochiella asaccharolytica, or Gordonibacterium pamelaeae (398,399).…”

Section: Resultsmentioning

confidence: 99%

“…Currently, we have cultured 41 different T. whipplei strains from diverse tissues and fluid (310,312,374,375,390,391), playing a key role in the understanding of the clinical manifestations caused by this bacterium (194). Coculture with amoebae, which was performed in our laboratory, has allowed us to identify 139 different bacterial species from both clinical and environmental samples, including 10 new bacterial species (224,235,239,251,261,(392)(393)(394)(395)(396). In our laboratory, the great qualities of several "cultivators," technicians, or researchers were identified and recognized in the names of bacterial species to honor these qualities (i.e., Rickettsia raoultii, Legionella drancourtii, Mycobacterium barrassiae, Afipia birgiae, Bosea vestrisii, and Bosea eneae, etc.…”

Section: Resultsmentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

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“…Catalase activity was determined on freshly grown colonies using 3 % (v/v) hydrogen peroxide solution. Determination of starch and casein hydrolysis and oxidative or fermentative catabolism followed the methods described by Smibert & Krieg (1994) and La Scola et al (2003). Growth with various pH, temperature and sodium chloride regimes was determined on LB agar plates incubated for up to 3 days.…”

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confidence: 99%

Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga

Zhang

Song

Xin

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga A Gram-negative-staining bacterium, designated strain 25B T , was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25B T grew optimally at 37 6C and pH 7.0. The predominant fatty acids of this soil isolate were C 18 : 1 v7c, C 19 : 0 cyclo v8c and C 16 : 0 . Phylogenetic analysis based on the almostcomplete 16S rRNA gene sequence showed that strain 25B T formed a monophyletic clade with the type strain of Microvirga subterranea; the two organisms shared 97.2 % 16S rRNA gene sequence similarity. However, the two strains shared low DNA-DNA relatedness. Strain 25B T was also readily distinguishable from Microvirga subterranea DSM 14364 T by various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus Microvirga, for which the name Microvirga guangxiensis sp. nov. is proposed. The type strain is 25B T (5CGMCC 1.7666 T 5JCM 15710 T ).The genus Microvirga was established by Kanso & Patel (2003) to accommodate strictly aerobic, Gram-negative, non-sporulating, motile and rod-shaped bacteria from free-flowing geothermal waters of a bore tapping the Great Artesian Basin of Australia, which grew optimally at 41 u C and pH 7.0 and had an absolute requirement for yeast extract. The genus currently encompasses a single species with a validly published name, Microvirga subterranea, and is assigned to the class Alphaproteobacteria, phylum Proteobacteria (Garrity & Holt, 2001;Garrity et al., 2005a), almost equidistant from Methylobacterium species (Patt et al., 1976;Urakami et al., 1993) Egli et al., 1988) and Bosea thiooxidans (Das et al., 1996), its nearest phylogenetic relatives, with a mean similarity value of 93 %., Chelatococcus asaccharovorans (AulingA sampling campaign during an ecological survey of soil bacteria in China led to the isolation of a pink-pigmented bacterium, designated strain 25B T . The soil sample (10 cm in depth, pH 6.2) was collected from a rice field in Guangxi Province. A glucose-yeast extract-malt extract (GYM) agar plate (comprising, l 21 : 4 g D-glucose, 4 g yeast extract, 10 g malt extract, 15 g agar; pH 7.3) was inoculated with a suspension of the soil and incubated at 37 u C for 7 days. The isolate that was obtained, strain 25B T , was maintained on LB agar (l 21 : 10 g tryptone, 5 g yeast extract, 10 g NaCl, 15 g agar; pH 7.0) at 4 u C and as glycerol suspensions (20 %, v/v) at 220 u C. Biomass for the chemotaxonomic and molecular systematic studies was prepared as described previously (Zhang et al., 2002) with the modification that the strain was grown in shake flasks of LB broth.Micromorphological and colonial properties of strain 25B T were examined on LB agar, Rourf's agar (Mulder & Deinema, 1992) and ...

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Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)

Cited by 81 publications

References 17 publications

Microorganisms Resistant to Free-Living Amoebae

Microorganisms Resistant to Free-Living Amoebae

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga

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