Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester

Ouattara, Aboubakar S.; Assih, Essokazi A.; Thierry, Sébastien; Cayol, Jean‐Luc; Labat, Marc; Monroy, O.; Macarie, Hervé

doi:10.1099/ijs.0.02540-0

Cited by 49 publications

(27 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The other related species are mesophilic bacteria, and no growth was detected at 45°C in case of B. minatitlanensis DSM 13099 T or B. thiooxidans DSM 9653 T (Ouattara et al 2003). Also none of the related strains identified by the assays using API 20 NE or BBLÔ Oxi ⁄ Ferm Tube II tests was recorded to be a thermophilic bacterium.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Ibrahim

Willems

Steinbüchel

2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: This study aimed at isolating thermophilic bacteria that utilize cheap carbon substrates for the economically feasible production of poly(3-hydroxybutyrate), poly(3HB), at elevated temperatures. Methods and Results: Thermophilic bacteria were enriched from an aerobic organic waste treatment plant in Germany, and from hot springs in Egypt. Using the viable colony staining method for hydrophobic cellular inclusions with Nile red in mineral salts medium (MSM) containing different carbon sources, six Gram-negative bacteria were isolated. Under the cultivation conditions used in this study, strains MW9, MW11, MW12, MW13 and MW14 formed stable star-shaped cell-aggregates (SSCAs) during growth; only strain MW10 consisted of free-living rod-shaped cells. The phylogenetic relationships of the strains as derived from 16S rRNA gene sequence comparisons revealed them as members of the Alphaproteobacteria. The 16S rRNA gene sequences of the isolates were very similar (> 99% similarity) and exhibited similarities ranging from 93 to 99% with the most closely related species that were Chelatococcus daeguensis, Chelatococcus sambhunathii,Chelatococcus asaccharovorans, Bosea minatitlanensis, Bosea thiooxidans and Methylobacterium lusitanum. Strains MW9, MW10, MW13 and MW14 grew optimally in MSM with glucose, whereas strains MW11 and MW12 preferred glycerol as sole carbon source for growth and poly(3HB) accumulation. The highest cell density and highest poly(3HB) content attained were 4 center dot 8 g l-l (cell dry weight) and 73% (w/w), respectively. Cells of all strains grew at temperatures between 37 and 55 degrees C with the optimum growth at 50 degrees C. Conclusions: New PHA-accumulating thermophilic bacterial strains were isolated and characterized to produce poly(3HB) from glucose or glycerol in MSM at 50 degrees C. SSCAs formation was reported during growth. Significance and Impact of the Study: To the best of our knowledge, this is the first report on the formation of SSCAs by PHA-accumulating bacteria and also by thermophilic bacteria. PHA-producing thermophiles can significantly reduce the costs of fermentative PHA production

show abstract

Section: Discussionmentioning

confidence: 99%

“…The other related species are mesophilic bacteria, and no growth was detected at 45°C in case of B. minatitlanensis DSM 13099 T or B. thiooxidans DSM 9653 T (Ouattara et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Ibrahim

Willems

Steinbüchel

2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Among the Bradyrhizobiaceae, bacteria of the genera Bosea and Afipia were the most frequently isolated. Due to the fastidiousness of these bacteria (3,15,16), identification is mostly based on 16S rRNA gene sequence (15,16,21). However, the 16S rRNA genes of these bacteria show very low variability: bacteria with only 1 base difference may belong to different species, as evidenced by DNA-DNA hybridization studies (15,16,25).…”

mentioning

confidence: 99%

Usefulness of rpoB Gene Sequencing for Identification of Afipia and Bosea Species, Including a Strategy for Choosing Discriminative Partial Sequences

Khamis

Colson

Raoult

et al. 2003

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacteria belonging to the genera Afipia and Bosea are amoeba-resisting bacteria that have been recently reported to colonize hospital water supplies and are suspected of being responsible for intensive care unitacquired pneumonia. Identification of these bacteria is now based on determination of the 16S ribosomal DNA sequence. However, the 16S rRNA gene is not polymorphic enough to ensure discrimination of species defined by DNA-DNA relatedness. The complete rpoB sequences of 20 strains were first determined by both PCR and genome walking methods. The percentage of homology between different species ranged from 83 to 97% and was in all cases lower than that observed with the 16S rRNA gene; this was true even for species that differed in only one position. The taxonomy of Bosea and Afipia is discussed in light of these results. For strain identification that does not require the complete rpoB sequence (4,113 to 4,137 bp), we propose a simple computerized method that allows determination of nucleotide positions of high variability in the sequence that are bordered by conserved sequences and that could be useful for design of universal primers. A fragment of 740 to 752 bp that contained the most highly variable area (positions 408 to 420) was amplified and sequenced with these universal primers for 47 strains. The variability of this sequence allowed identification of all strains and correlated well with results of DNA-DNA relatedness. In the future, this method could be also used for the determination of variability "hot spots" in sets of housekeeping genes, not only for identification purposes but also for increasing the discriminatory power of sequence typing techniques such as multilocus sequence typing.Aquatic bacteria such as Legionella, Pseudomonas, Stenotrophomonas, Burkholderia spp., and Acinetobacter spp. may colonize hospital water supplies and have previously been shown to be causally associated with cases of nosocomial infections (23). Free-living amoebae have been shown to be a reservoir of pathogens, such as Legionella spp., Burkholderia pickettii, and Cryptococcus neoformans (2, 26). The most studied of amoebaresisting bacteria (ARB) is Legionella pneumophila, the agent of Legionnaires' disease (27), which frequently results from exposure to contaminated aerosols. There are growing hints that additional ARB might be implicated in community-acquired pneumonia, including Legionella-like amoebal pathogens and members of the genus Parachlamydia (19). As part of the research into the diversity of bacterial agents associated with amoebae in hospital water supplies, we previously identified new ␣-proteobacteria belonging to the Bradyrhizobiaceae (13). Moreover, we demonstrated that patients with nosocomial pneumonia who were hospitalized in a public hospital where contaminated water was found had elevated titers of antibodies against these bacteria (14) and that patient seroconversion to Bosea massiliensis was frequent in patients hospitalized in intensive care units and was associated with the occurrence ...

show abstract

“…Various morphological, biochemical, and molecular traits (DNA-DNA hybridization analysis) determined the isolation of four more species in this genus, B. eneae, B. vestrisii, B. massiliensis, and B. minatitlanensis (La Scola et al 2003; Ouattara et al 2003), and the additional isolates Bosea lupine, Bosea lathyri, and Bosea robiniae were determined by multilocus analysis techniques .…”

Section: Phenotypic Analysesmentioning

confidence: 99%

The Family Bradyrhizobiaceae

et al. 2014

View full text Add to dashboard Cite

Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester

Cited by 49 publications

References 11 publications

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Usefulness of rpoB Gene Sequencing for Identification of Afipia and Bosea Species, Including a Strategy for Choosing Discriminative Partial Sequences

The Family Bradyrhizobiaceae

Contact Info

Product

Resources

About