Botanical and genetic characters of Erythrina × neillii cultivated in Egypt

Abstract

a b s t r a c tErythrina × neillii Mabberley & Lorence, Fabaceae, is a sterile hybrid between E. herbacea L. and E. humeana Spreng. Nothing was traced about its genetic, macro and micromorphology. Therefore, it was deemed of interest to study its botanical characters, in addition to the DNA fingerprint to help in the identification of the plant. The anatomical characters of the old stem and its bark are characterized by the presence of cork cells, bast fibers and sclereids. Pericycle is sclerenchymatous forming crystal sheath. The epidermises of the leaf and young stem are characterized by the presence of anomocytic and paracytic stomata, nonglandular, unicellular and multicellular two armed hairs, and glandular club shaped hair. Calcium oxalate is present in the form of crystal sheath and prisms. Secretory cavities are distributed in the phloem and cortex. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used as one of the molecular methods to differentiate between the samples of Erythrina. The DNA of Erythrina was extracted and analyzed using seven-mer random primers. Randomly Amplified Polymorphic DNA were recognized. This characterization allows certification of the authenticity of Erythrina × neillii, in order to provide quality control for the plant.

Phytotomy of ten accessions of Vigna subterranean (L.) Verdc

“…In this study, the stem anatomical investigations revealed that the cortex is made of parenchyma and sclerenchyma cells, vascular bundles varied among accessions. Also Vascular tissues alternate with the vascular bundles for all the accessions studied considering the type of vascular bundles found in the stem and petiole of the ten accessions studied (collateral vascular bundles), the same were found in Erythrina velutina of Fabaceae family [29].…”

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“…The unique observation of flower-like cell arrangements in the pith region of the stem can be considered as a distinguishing feature. Uni, bi or multiseriate medullary rays extended towards phloem region may also be considered for good diagnostic characters for plant discrimination 30 . Starch grains found in plants have characteristic morphological features, physical and chemical properties, which may be used as diagnostic characters to identify the plant species 31 .…”