Botanical survey and leishmanicidal activity of grown-love

Abstract

Grown-love (Portulaca pilosa L) is used in Amazon medicine for the treatment of diseases caused by bacteria, wound care. The objective of this work was to conduct a botanical survey of P. pilosa, as well as to evaluate its leishmanicidal activity. For the botanical studies techniques of epidermal decoupling and histological sections were used (by hand and microtome). The ethanol extract (EE) of aerial parts of P. pilosa was obtained by maceration, followed by concentration in evaporator route. The EE was fractionated by chromatographic column. The EE and fractions were analyzed in thin layer chromatography (TLC) and NMR. The leishmanicidal activity was evaluated against promastigotes and amastigotes of Leishmania amazonensis, and promastigotes of L. chagasi, with prior cytotoxicity assessment in murine macrophages. In anatomical terms, P. pilosa presents leaf surface with adaxial epidermal cells and sinuous walls. In the phytochemical prospection of the extract, terpenes and phenols were detected, among others. The analysis of the NMR suggested the presence of terpenes. All samples were inactive against promastigotes and amastigotes of L. (L) amazonensis. The dichloromethane fraction was active against promastigotes of L. (L) chagasi. The EE, dichloromethane, ethyl acetate and methanol fractions showed no cytotoxicity to murine peritoneal macrophages (CC50> 500 mg/mL). The dichloromethane fraction was promising for Leishmania, so futher studies may lead to the isolation of the active metabolite.

Extractos y componentes fitoquímicos de Clibadium remotiflorum, Lonchocarpus nicou, Hura crepitans, Dieffenbachia costata, con probable valor terapéutico contra Leishmania, in vitro, San Martín

“…Así mismo utilizando macrófagos murinos para evaluar la toxicidad de los alcaloides, observaron que no eran tóxicos (15); aun siendo citotóxico estos alcaloides, son menos potentes que el glucantime. Similarmente la actividad leishmanicida fue evaluada contra promastigotos de Leishmania amazonensis con pobre citotoxicidad, evaluados en macrófagos (19). El extracto fluido de hojas de Tessaria integrifolia presenta actividad leishmanicida sobre Mesocricetus auratus (20).…”

Decoration of Ultramicrotome-Cut Polymers with Silver Nanoparticles: Effect of Post-Deposition Laser Treatment

“…The microtome is an original mechanical instrument that serves to prepare very thin cuts of biological samples for microscopic examination [5]. However, it has been used effectively to cut, for example, botanical specimens [6] and newly developed polymers [7]. Microtome cuts in the preparation of botanical specimens refer to various parts of botanical tissues, very small sections of wood [5] and plants [6], which serve for histological and pathological studies.…”

“…However, it has been used effectively to cut, for example, botanical specimens [6] and newly developed polymers [7]. Microtome cuts in the preparation of botanical specimens refer to various parts of botanical tissues, very small sections of wood [5] and plants [6], which serve for histological and pathological studies. Brandão et al [6] used a microtome to cut the aerial parts of the Portulaca pilosa L. plant used in Amazon medicine for the treatment of bacteria-related diseases.…”