Both Hsp70 chaperone and Clp protease plastidial systems are required for protection against oxidative stress

Pulido, Pablo; Llamas, Ernesto; Rodríguez‐Concepción, Manuel

doi:10.1080/15592324.2017.1290039

Cited by 45 publications

(25 citation statements)

References 34 publications

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“…Paraquat is widely used to study the effects of oxidative stress to isolate mutants and investigate the functions of genes involved in ROS detoxification pathways [7,[9][10][11][12][13]. In addition to SODs and other antioxidant enzymes that are part of the elaborate antioxidant defense network, plants employ various molecular strategies to counteract oxidative stress and minimize its detrimental effect, including the accumulation of osmoprotectants and compatible solutes, stress protective proteins such as late embryogenesis abundant (LEA) proteins/dehydrins, heat shock proteins (HSPs), and an activation of cellular repair mechanisms [8,14,15].…”

Section: Introductionmentioning

confidence: 99%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.

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Section: Introductionmentioning

confidence: 99%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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“…Interestingly, HSP70 was also identified among the proteins that were more abundant in seeds of the tolerant hybrid (Table ). HSPs 70 are chaperone proteins that bind ATP in response to stress, and they are also involved in protection against oxidative stress (Pulido, Llamas & Rodriguez‐Concepcion ). In cotton fibre, it has been demonstrated that their inhibition leads to H 2 O 2 accumulation and oxidative stress (Sable et al, ).…”

Section: Discussionmentioning

confidence: 99%

A multiscale approach reveals regulatory players of water stress responses in seeds during germination

Saux

Ponnaiah

Langlade

et al. 2020

Plant Cell & Environment

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Seed germination is regulated by environmental factors, particularly water availability. Water deficits at the time of sowing impair the establishment of crop plants.Transcriptome and proteome profiling was used to document the responses of sunflower (Helianthus annuus) seeds to moderate water stress during germination in two hybrids that are nominally classed as drought sensitive and drought tolerant. Differences in the water stress-dependent accumulation reactive oxygen species and antioxidant enzymes activities were observed between the hybrids. A pathway-based analysis of the hybrid transcriptomes demonstrated that the water stress-dependent responses of seed metabolism were similar to those of the plant, with a decreased abundance of transcripts encoding proteins associated with metabolism and cell expansion. Moreover, germination under water stress conditions was associated with increased levels of transcripts encoding heat shock proteins. Exposure of germinating seeds to water stress specifically affected the abundance of a small number of proteins, including heat shock proteins. Taken together, these data not only identify factors that are likely to play a key role in drought tolerance during seed germination, but they also demonstrate the importance of the female parent in the transmission of water stress tolerance.

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“…Inactivation of the ClpC1 gene in A. thaliana reduced plant growth and hampered chloroplast development [135][136][137]. When mutants with different variations of Clp protease (clpr1, clps, clpc1, clpc1, clpd, clpt1, and clpt2) were treated with methyl viologen, clpc1 and clpc2 mutants were more resistant to methyl viologen, compared to other mutants [138]. clpc1 and clpc2 mutants show 90% similarity in DNA and amino acid sequences.…”

Section: Roles Of Clp Protease Complexes In Land Plants During Stressmentioning

confidence: 99%

Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion

Ali

Baek

2020

Plants

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Protein malfunction is typically caused by abiotic stressors. To ensure cell survival during conditions of stress, it is important for plant cells to maintain proteins in their respective functional conformation. Self-compartmentalizing proteases, such as ATP-dependent Clp proteases and proteasomes are designed to act in the crowded cellular environment, and they are responsible for degradation of misfolded or damaged proteins within the cell. During different types of stress conditions, the levels of misfolded or orphaned proteins that are degraded by the 26S proteasome in the cytosol and nucleus and by the Clp proteases in the mitochondria and chloroplasts increase. This allows cells to uphold feedback regulations to cellular-level signals and adjust to altered environmental conditions. In this review, we summarize recent findings on plant proteolytic complexes with respect to their protective functions against abiotic and biotic stressors.

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Both Hsp70 chaperone and Clp protease plastidial systems are required for protection against oxidative stress

Cited by 45 publications

References 34 publications

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

A multiscale approach reveals regulatory players of water stress responses in seeds during germination

Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion

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