Both viable and inactivated amoeba spores protect their intracellular bacteria from drinking water disinfection

He, Zhenzhen; Wang, Luting; Ge, Yuexian; Zhang, Siyi; Tian, Yuehui; Yang, Xin; Shu, Longfei

doi:10.1016/j.jhazmat.2021.126006

Cited by 35 publications

(37 citation statements)

References 55 publications

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“…In contrast to FLA, the BCA spores infected by B. agricolaris exhibited different colors under fluorescent light. Such differences were used to differentiate the spores of BCA and FLA. 29,46 Consistent with other previously reported work, roughly 40% of the total prepared D. discoideum amoeba spores in the suspension were effectively infected by B. agricolaris (Figure S7).…”

Section: ■ Materials and Methodssupporting

confidence: 91%

“…28 In particular, exposure to 100 mg/L chlorine dioxide for 10 min or 0.5 mg/L only has a limited impact on deactivating amoeba spores. 29,30 These reports collectively suggest that the fate and transport of amoebae in environments can lead to the development of virulence in human-pathogenic microorganisms. 27 Therefore, this area requires further exploration.…”

Section: ■ Introductionmentioning

confidence: 99%

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Host–Endosymbiont Relationship Impacts the Retention of Bacteria-Containing Amoeba Spores in Porous Media

Jin

Zhao

et al. 2022

Environ. Sci. Technol.

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Amoebae are protists that are commonly found in water, soil, and other habitats around the world and have complex interactions with other microorganisms. In this work, we investigated how host−endosymbiont interactions between amoebae and bacteria impacted the retention behavior of amoeba spores in porous media. A model amoeba species, Dictyostelium discoideum, and a representative bacterium, Burkholderia agricolaris B1qs70, were used to prepare amoeba spores that carried bacteria. After interacting with B. agricolaris, the retention of D. discoideum spores was enhanced compared to noninfected spores. Diverse proteins, especially proteins contributing to the looser exosporium structure and cell adhesion functionality, are secreted in higher quantities on the exosporium surface of infected spores compared to that of noninfected ones. Comprehensive examinations using a quartz crystal microbalance with dissipation (QCM-D), a parallel plate chamber, and a single-cell force microscope present coherent evidence that changes in the exosporium of D. discoideum spores due to infection by B. agricolaris enhance the connections between spores in the suspension and the spores that were previously deposited on the collector surface, thus resulting in more retention compared to the uninfected ones in porous media. This work provides novel insight into the retention of amoeba spores after bacterial infection in porous media and suggests that the host−endosymbiont relationship regulates the fate of biocolloids in drinking water systems, groundwater, and other porous environments.

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Section: ■ Materials and Methodssupporting

confidence: 91%

Section: ■ Introductionmentioning

confidence: 99%

Host–Endosymbiont Relationship Impacts the Retention of Bacteria-Containing Amoeba Spores in Porous Media

Jin

Zhao

et al. 2022

Environ. Sci. Technol.

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“…The amoeba proto-farming symbiosis is a promising system to address whether unicellular protist hosts could recognize and discriminate their symbionts ( 19 – 21 , 25 – 28 ). Dictyostelium discoideum is a soil-dwelling amoeba belonging to protozoa and primarily feeding on bacteria, which has been widely used as an ideal system to study cell biology, symbiosis, evolution, and ecology ( 20 , 21 , 23 , 24 , 26 , 28 – 32 ). Amoebas can aggregate and differentiate into pluricellular fruiting bodies upon food-deprived conditions.…”

Section: Introductionmentioning

confidence: 99%

Symbiont-Induced Phagosome Changes Rather than Extracellular Discrimination Contribute to the Formation of Social Amoeba Farming Symbiosis

Tian

Peng

et al. 2022

Microbiol Spectr

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“…Meanwhile, some bacteria have evolved various mechanisms to escape or resist protistan predation . Bacteria developed many tools to weaken prey encounter and recognition, reducing the attack rate and the predation pressure .…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, some bacteria have evolved various mechanisms to escape or resist protistan predation. 8 Bacteria developed many tools to weaken prey encounter and recognition, reducing the attack rate and the predation pressure. 9 The bacterial cell wall traits (e.g., flagella and cell wall components), bacterial secretion, and oxidative stress response determine the success of protist predation on bacteria.…”

Section: ■ Introductionmentioning

confidence: 99%

Soil Amoebae Affect Iron and Chromium Reduction through Preferential Predation between Two Metal-Reducing Bacteria

Huang

et al. 2022

Environ. Sci. Technol.

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Soil protists are essential but often overlooked in soil and could impact microbially driven element cycling in natural ecosystems. However, how protists influence heavy metal cycling in soil remains poorly understood. In this study, we used a model protist, Dictyostelium discoideum, to explore the effect of interactions between soil amoeba and metal-reducing bacteria on the reduction of soil Fe(III) and Cr(VI). We found that D. discoideum could preferentially prey on the Fe(III)-reducing bacterium Shewanella decolorationis S12 and significantly decrease its biomass. Surprisingly, this predation pressure also stimulated the activity of a single S. decolorationis S12 bacterium to reduce Fe(III) by enhancing the content of electron-transfer protein cyt c, intracellular ATP synthesis, and reactive oxygen species (e.g., H2O2). We also found that D. discoideum could not prey on the Cr(VI)-reducing bacterium Brevibacillus laterosporus. In contrast, B. laterosporus became edible to amoebae in the presence of S. decolorationis S12, and their Cr(VI) reduction ability decreased under amoeba predation pressure. This study provides direct evidence that protists can affect the Cr and Fe cycling via the elective predation pressure on the metal-reducing bacteria, broadening our horizons of predation of protists on soil metal cycling.

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Both viable and inactivated amoeba spores protect their intracellular bacteria from drinking water disinfection

Cited by 35 publications

References 55 publications

Host–Endosymbiont Relationship Impacts the Retention of Bacteria-Containing Amoeba Spores in Porous Media

Host–Endosymbiont Relationship Impacts the Retention of Bacteria-Containing Amoeba Spores in Porous Media

Symbiont-Induced Phagosome Changes Rather than Extracellular Discrimination Contribute to the Formation of Social Amoeba Farming Symbiosis

Soil Amoebae Affect Iron and Chromium Reduction through Preferential Predation between Two Metal-Reducing Bacteria

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