Bothrops leucurus snake venom protein profile, isolation and biological characterization of its major toxin PLA2s-like

Dias, Êuder Reis; Oliveira, Laudicéia Alves de; Lauria, Pedro Santana Sales; Bordon, Karla de Castro Figueiredo; Domênico, Adriana Moraes Rodrigues; Guerreiro, Marcos Lázaro da Silva; Wiezel, Gisele Adriano; Cardoso, Iara Aimê; Rossini, Bruno César; Marino, Celso Luís; Pimenta, Daniel Carvalho; Arantes, Eliane Candiani; Casais-e-Silva, Luciana Lyra; Branco, Alexsandro; Santos, Lucilene Delazari dos; Biondi, Ilka

doi:10.1016/j.toxicon.2022.04.002

Cited by 5 publications

(2 citation statements)

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“…In this study, the authors indicate that a decomplexation step, although being time and labor consuming, provides higher coverage of proteins [110]. In another study, Bothrops leucurus venom yielded 137 proteins hits for shotgun analysis compared to 62 proteins identified using the first workflow [114].…”

Section: Whole Proteome Characterization and Identificationmentioning

confidence: 81%

“…Shotgun analysis has numerous advantages over other techniques, since it does not require a decomplexation process at the beginning that is time consuming and, in some cases, expensive. This strategy allows the recovery of low abundant proteins, since they may be lost during the separation step performed in other techniques [114]. This is reflected with a relatively high number of protein hits compared to other techniques.…”

Section: Whole Proteome Characterization and Identificationmentioning

confidence: 99%

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Separation and Analytical Techniques Used in Snake Venomics: A Review Article

et al. 2022

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The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms are considered as libraries of bioactive molecules that can be used to develop innovative drugs. Numerous separation and analytical techniques are combined to study snake venom composition including chromatographic techniques such as size exclusion and RP-HPLC and electrophoretic techniques. Herein, we present in detail these existing techniques and their applications in snake venom research. In the first part, we discuss the different possible technical combinations that could be used to isolate and purify SV proteins using what is known as bioassay-guided fractionation. In the second part, we describe four different proteomic strategies that could be applied for venomics studies to evaluate whole venom composition, including the mostly used technique: RP-HPLC. Eventually, we show that to date, there is no standard technique used for the separation of all snake venoms. Thus, different combinations might be developed, taking into consideration the main objective of the study, the available resources, and the properties of the target molecules to be isolated.

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Section: Whole Proteome Characterization and Identificationmentioning

confidence: 81%

Section: Whole Proteome Characterization and Identificationmentioning

confidence: 99%