Bothrops moojeni myotoxin-II, a Lys49-phospholipase A2 homologue: An example of function versatility of snake venom proteins

Stábeli, Rodrigo G.; Amui, Saulo França; Sant’Ana, Carolina D.; Pires, Matheus Giovanni; Nomizo, Auro; Monteiro, Marta Chagas; Romão, Pedro Roosevelt Torres; Guerra‐Sá, Renata; Vieira, Camila; Giglio, José Roberto; Fontes, Marcos R.M.; Soares, Andreimar M.

doi:10.1016/j.cbpc.2005.11.020

Cited by 79 publications

(73 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Solutions of B. jararacussu snake venom BthTX-I PLA 2 (M r 14,088.1), chicken egg ovalbumin (M r 44,400.0) and bovine serum albumin (BSA, M r 66,430.0) were used to calibrate the mass scale. The mass values assigned to the amino acid residues represent average values [24].…”

Section: Biochemical Characterizationmentioning

confidence: 99%

Cytotoxic l-amino acid oxidase from Bothrops moojeni: Biochemical and functional characterization

Stábeli

Sant’Ana

Ribeiro

et al. 2007

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

Section: Biochemical Characterizationmentioning

confidence: 99%

Cytotoxic l-amino acid oxidase from Bothrops moojeni: Biochemical and functional characterization

Stábeli

Sant’Ana

Ribeiro

et al. 2007

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

“…Various venoms and toxins show antibacterial activity, such as miotoxin II from B. moojeni and venom from B. neuwiedi pauloensis (8,9). Several bothropic venoms inhibit growth of some intracellular parasites, such as Leishmania sp.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom

Torres¹,

Dantas²,

Menezes³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi

show abstract

“…These toxins are pure chemical substances that are present in venoms presenting specific action in biological systems [9]. Several studies demonstrate the antimicrobial potential of venoms of neotropical snakes, especially those from the genus Bothrops [10]. Bothrops toxin-II (BthTX-II) used in this study was isolated from Bothrops jararacussu venom, which also displays myotoxic action, whose amino acid sequence was further characterized and it was identified as a basic Asp49-PLA 2 [11].…”

Section: Introductionmentioning

confidence: 99%

Liposomes containing an ASP49-phospholipase A 2 from Bothrops jararacussu snake venom as experimental therapy against cutaneous leishmaniasis

Barros

Macedo

Ferreira

et al. 2016

International Immunopharmacology

Self Cite

View full text Add to dashboard Cite

Bothrops moojeni myotoxin-II, a Lys49-phospholipase A2 homologue: An example of function versatility of snake venom proteins

Cited by 79 publications

References 53 publications

Cytotoxic l-amino acid oxidase from Bothrops moojeni: Biochemical and functional characterization

Cytotoxic l-amino acid oxidase from Bothrops moojeni: Biochemical and functional characterization

Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom

Liposomes containing an ASP49-phospholipase A 2 from Bothrops jararacussu snake venom as experimental therapy against cutaneous leishmaniasis

Contact Info

Product

Resources

About