Botrytis cinerea

Chen, Tong; Zhang, Zhanquan; Chen, Yong; Li, Boqiang; Tian, Shiping

doi:10.1016/j.cub.2023.01.058

Cited by 20 publications

(4 citation statements)

References 8 publications

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“…As a result, growers adopt integrated management strategies to minimize crop losses. These strategies combine multiple approaches such as cultural practices, chemical and biological controls, and use of tolerant/resistant varieties (Chen et al., 2023 ; Hua et al., 2018 ; Orozco‐Mosqueda et al., 2023 ). Because the quantitative nature of host resistance confounds breeding efforts, chemical fungicides are the primary means of control.…”

Section: Emerging Strategies For Disease Control Of B Cinereamentioning

confidence: 99%

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Singh,

Caseys,

Kliebenstein

2023

Molecular Plant Pathology

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Botrytis cinerea Pers. Fr. (teleomorph: Botryotinia fuckeliana) is a necrotrophic fungal pathogen that attacks a wide range of plants. This updated pathogen profile explores the extensive genetic diversity of B. cinerea, highlights the progress in genome sequencing, and provides current knowledge of genetic and molecular mechanisms employed by the fungus to attack its hosts. In addition, we also discuss recent innovative strategies to combat B. cinerea.TaxonomyKingdom: Fungi, phylum: Ascomycota, subphylum: Pezizomycotina, class: Leotiomycetes, order: Helotiales, family: Sclerotiniaceae, genus: Botrytis, species: cinerea.Host rangeB. cinerea infects almost all of the plant groups (angiosperms, gymnosperms, pteridophytes, and bryophytes). To date, 1606 plant species have been identified as hosts of B. cinerea.Genetic diversityThis polyphagous necrotroph has extensive genetic diversity at all population levels shaped by climate, geography, and plant host variation.PathogenicityGenetic architecture of virulence and host specificity is polygenic using multiple weapons to target hosts, including secretory proteins, complex signal transduction pathways, metabolites, and mobile small RNA.Disease control strategiesEfforts to control B. cinerea, being a high‐diversity generalist pathogen, are complicated. However, integrated disease management strategies that combine cultural practices, chemical and biological controls, and the use of appropriate crop varieties will lessen yield losses. Recently, studies conducted worldwide have explored the potential of small RNA as an efficient and environmentally friendly approach for combating grey mould. However, additional research is necessary, especially on risk assessment and regulatory frameworks, to fully harness the potential of this technology.

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Section: Emerging Strategies For Disease Control Of B Cinereamentioning

confidence: 99%

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Singh,

Caseys,

Kliebenstein

2023

Molecular Plant Pathology

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“…B. cinerea has a remarkably wide host range and infects more than 1000 plant species, including almost all fruit and vegetable crops (Yigal et al 2016 ). Moreover, B. cinerea attacks horticultural crops not only in the field but also during postharvest storage, causing an annual economic loss of over $10 billion (Chen et al 2023b ). Due to its substantial economic impact and scientific importance, B. cinerea ranks second among the top ten plant fungal pathogens worldwide (Dean et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Zhang,

Chen

et al. 2024

Mol Horticulture

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Botrytis cinerea is one of the most destructive phytopathogenic fungi, causing significant losses to horticultural crops. As a necrotrophic fungus, B. cinerea obtains nutrients by killing host cells. Secreted cell death-inducing proteins (CDIPs) play a crucial role in necrotrophic infection; however, only a limited number have been reported. For high-throughput CDIP screening, we optimized the prokaryotic expression system and compared its efficiency with other commonly used protein expression systems. The optimized prokaryotic expression system showed superior effectiveness and efficiency and was selected for subsequent CDIP screening. The screening system verified fifty-five candidate proteins and identified two novel SGNH family CDIPs: BcRAE and BcFAT. BcRAE and BcFAT exhibited high expression levels throughout the infection process. Site-directed mutagenesis targeting conserved Ser residues abolished the cell death-inducing activity of both BcRAE and BcFAT. Moreover, the transient expression of BcRAE and BcFAT in plants enhanced plant resistance against B. cinerea without inducing cell death, independent of their enzymatic activities. Our results suggest a high-efficiency screening system for high-throughput CDIP screening and provide new targets for further study of B. cinerea-plant interactions.

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“…Botrytis cinerea is a ubiquitous plant pathogenic fungus that infects over 1,400 plant species. It is the primary cause of post-harvest losses of fruits and vegetables, resulting in significant yield reductions in global agricultural production ( 1 – 3 ). The conidia of B. cinerea attach to and germinate on plant surfaces, subsequently differentiate into germ tubes or elongated hyphae, which then form simple appressoria or multicellular infection cushions, which invade the host tissue by releasing diverse virulence factors ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

The GATA transcription factor BcWCL2 regulates citric acid secretion to maintain redox homeostasis and full virulence in Botrytis cinerea

Ren,

Qian,

Ren

et al. 2024

mBio

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Botrytis cinerea is a typical necrotrophic plant pathogenic fungus which can deliberately acidify host tissues and trigger oxidative bursts therein to facilitate its virulence. The white collar complex (WCC), consisting of BcWCL1 and BcWCL2, is recognized as the primary light receptor in B. cinerea . Nevertheless, the specific mechanisms through which the WCC components, particularly BcWCL2 as a GATA transcription factor, control virulence are not yet fully understood. This study demonstrates that deletion of BcWCL2 results in the loss of light-sensitive phenotypic characteristics. Additionally, the Δ bcwcl2 strain exhibits reduced secretion of citrate, delayed infection cushion development, weaker hyphal penetration, and decreased virulence. The application of exogenous citric acid was found to restore infection cushion formation, hyphal penetration, and virulence of the Δ bcwcl2 strain. Transcriptome analysis at 48 h post-inoculation revealed that two citrate synthases, putative citrate transporters, hydrolytic enzymes, and reactive oxygen species scavenging-related genes were down-regulated in Δ bcwcl2 , whereas exogenous citric acid application restored the expression of the above genes involved in the early infection process of Δ bcwcl2 . Moreover, the expression of Bcvel1 , a known regulator of citrate secretion, tissue acidification, and secondary metabolism, was down-regulated in Δ bcwcl2 but not in Δ bcwcl1 . ChIP-qPCR and electrophoretic mobility shift assays revealed that BcWCL2 can bind to the promoter sequences of Bcvel1 . Overexpressing Bcvel1 in Δ bcwcl2 was found to rescue the mutant defects. Collectively, our findings indicate that BcWCL2 regulates the expression of the global regulator Bcvel1 to influence citrate secretion, tissue acidification, redox homeostasis, and virulence of B. cinerea . IMPORTANCE This study illustrated the significance of the fungal blue light receptor component BcWCL2 protein in regulating citrate secretion in Botrytis cinerea . Unlike BcWCL1, BcWCL2 may contribute to redox homeostasis maintenance during infection cushion formation, ultimately proving to be essential for full virulence. It is also demonstrated that BcWCL2 can regulate the expression of Bcvel1 to influence host tissue acidification, citrate secretion, infection cushion development, and virulence. While the role of organic acids secreted by plant pathogenic fungi in fungus-host interactions has been recognized, this paper revealed the importance, regulatory mechanisms, and key transcription factors that control organic acid secretion. These understanding of the pathogenetic mechanism of plant pathogens can provide valuable insights for developing effective prevention and treatment strategies against fungal diseases.

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Botrytis cinerea

Cited by 20 publications

References 8 publications

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

The GATA transcription factor BcWCL2 regulates citric acid secretion to maintain redox homeostasis and full virulence in Botrytis cinerea

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