Bottle Cultivation of Culinary-Medicinal Bunashimeji Mushroom Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae) in Nagano Prefecture (Japan)

Nakamura, Kimiyoshi

doi:10.1615/intjmedmushr.v8.i2.90

Cited by 7 publications

(1 citation statement)

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“…자연계에서 분해자 역할을 하는 버섯은 예로부터 식용 및 약 용으로 널리 이용되어왔으며 [2,13,17,21], 버섯에 대한 연구 가 활발히 이루어진 결과, 항암활성, 면역활성, 항진균 활성, 항종양 효과 등을 가지는 여러 종류의 생리활성 물질들이 밝 혀졌다 [3,10,14,22]. 느티만가닥 [4,15]. 한국을 비롯한 동아시아 지역과 유럽에 주로 분포하고 있으며, 전세계적으로 1972년 이후 꾸준하게 생산량이 증가해왔다 [13].…”

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Secondary Structure of the Ribosomal Internal Transcribed Spacer (ITS) Region of Hypsizygus marmoreus

Woo¹,

Yoon²,

You³

et al. 2013

Journal of Life Science

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The ribosomal DNA (rDNA) clusters of Hypsizygus marmoreus 3-10 and H. marmoreus 1-1 were analyzed in this study. The small subunit (SSU) and intergenic spacer 2 (IGS 2) was partially sequenced. The internal transcribed spacer 1 (ITS 1), 5.8S, internal transcribed spacer 2 (ITS 2), large subunit (LSU), intergenic spacer 1 (IGS 1), and 5S were completely sequenced. The rDNA clusters of H. marmoreus 3-10 and H. marmoreus 1-1 were 7,049 bp in length. The sequence of SSU rDNA, which corresponded to 18S rDNA, was 1,796 bp in length, and the sequence of LSU rDNA, which corresponded to 28S rDNA, was 3,348 bp in length. The ITS region that variable region and IGS region that nontranscribed spacer was 462 bp and 1,290 bp in length. The sequence of 5.8S rDNA and 5S rDNA was 153 bp and 43 bp in length, respectively. The 17 bp of the rDNA cluster in the H. marmoreus 3-10 strain was different to that in the H. marmoreus 1-1 strain, with 2 bp in the SSU, 3 bp in the ITS, 9 bp in the LSU, and 3 bp in the IGS. The analysis of the secondary structure revealed that the ITS regions of H. marmoreus 3-10 and H. marmoreus 1-1 have five stem-loop structures. Interestingly, among these structures, one different nucleotide sequence resulted in a different secondary structure in stem-loop V.

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