Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications

Weisenberger, Mitchell S.; Deans, Tara L.

doi:10.1007/s10295-018-2027-3

Cited by 17 publications

(11 citation statements)

References 245 publications

(200 reference statements)

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“…Indeed, genetically engineered BMP expressing cells can release the growth factors in situ at the site of injury over extended periods of time at a physiological concentration [176]. To deliver the BMP-plasmids, the coupling with the material-based strategies allows generating BMP signaling over a specific period [177]. This can be accomplished by assembling functional genetic modules to control intrinsic gene expression patterns and similarly, assemble functional ECM domains to create custom-made microenvironments for directing cell behavior.…”

Section: Temporal Regulation Of Bmp Signalingmentioning

confidence: 99%

Learning from BMPs and their biophysical extracellular matrix microenvironment for biomaterial design

Migliorini

Guevara-Garcia

Albigès-Rizo

et al. 2020

Bone

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It is nowadays well-accepted that the extracellular matrix (ECM) is not a simple reservoir for growth factors but is an organization center of their biological activity. In this review, we focus on the ability of the ECM to regulate the biological activity of BMPs. In particular, we survey the role of the ECM components, notably the glycosaminoglycans and fibrillary ECM proteins, which can be promoters or repressors of the biological activities mediated by the BMPs. We examine how a process called mechano-transduction induced by the ECM can affect BMP signaling, including BMP internalization by the cells. We also focus on the spatio-temporal regulation of the BMPs, including their release from the ECM, which enables to modulate their spatial localization as well as their local concentration. We highlight how biomaterials can recapitulate some aspects of the BMPs/ECM interactions and help to answer fundamental questions to reveal previously unknown molecular mechanisms. Finally, the design of new biomaterials inspired by the ECM to better present BMPs is discussed, and their use for a more efficient bone regeneration in vivo is also highlighted.

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Section: Temporal Regulation Of Bmp Signalingmentioning

confidence: 99%

Learning from BMPs and their biophysical extracellular matrix microenvironment for biomaterial design

Migliorini

Guevara-Garcia

Albigès-Rizo

et al. 2020

Bone

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“…[9] Synthetic techniques, specifically the incorporation of defined protein-protein interactions, have been used at the material level to influence conjugation and cell binding. [10] These have been developed to create smart materials that can dynamically respond to biological or physical cues to change material properties or function to influence cell behavior. [11] However, the opaque and non-specific relationships between materials and cell phenotype rely on physical or biological cues that initiate complex and multicomponent intracellular signaling pathways that activate sets of genes for cell differentiation or phenotypic changes.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology and Tissue Engineering: Toward Fabrication of Complex and Smart Cellular Constructs

Hoffman

Antovski

Tebon

et al. 2020

Adv Funct Materials

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Tissue engineering approaches, with the goals of replacing or recovering damaged or diseased tissues, or of reconstituting tissues in vitro for disease modeling and drug development, have the potential to make significant contributions to medicine. Advances in stem cell biology, biomaterial synthesis and characterization, and microscale technologies have made engineered tissues a reality. However, the classic tools used to build tissues in the lab do not allow for complete control of cell behaviors. More recently, synthetic biology principles have developed robust and versatile approaches to program cells with artificial genetic circuits, where cell behavior and function can be manipulated. At the interface between synthetic biology and tissue engineering, there is space for a new area of investigation where material engineering and cellular engineering complement and sustain each other. In this progress report, synthetic biology principles and how they have been used to engineer cells with potential to dictate cell behavior and function in tissue constructs of the future are briefly described. It is our belief that this research area still needs further exploration to fully exploit synthetic biology to make smart and functional cellular constructs for therapeutic and in vitro applications.Received: ((will be filled in by the editorial staff))Revised: ((will be filled in by the editorial staff))

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“…Novel genetic tools built by synthetic biologists have transformed how cells can be reprogrammed and include genetic programs to introduce switching ( 7–14 ), oscillations ( 15–20 ), logic gates ( 21–23 ) and biosensing ( 24–30 ) behaviors into cells. Assembling simple genetic parts into more complex gene circuits can reliably and predictably control cell behaviors ( 31 , 32 ). To date, the majority of mammalian synthetic biology has taken place in easy-to-grow and easy-to-transfect cells, derived from immortalized cell lines.…”

Section: Introductionmentioning

confidence: 99%

Rosa26 docking sites for investigating genetic circuit silencing in stem cells

et al. 2020

Self Cite

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Approaches in mammalian synthetic biology have transformed how cells can be programmed to have reliable and predictable behaviour, however, the majority of mammalian synthetic biology has been accomplished using immortalized cell lines that are easy to grow and easy to transfect. Genetic circuits that integrate into the genome of these immortalized cell lines remain functional for many generations, often for the lifetime of the cells, yet when genetic circuits are integrated into the genome of stem cells gene silencing is observed within a few generations. To investigate the reactivation of silenced genetic circuits in stem cells, the Rosa26 locus of mouse pluripotent stem cells was modified to contain docking sites for site-specific integration of genetic circuits. We show that the silencing of genetic circuits can be reversed with the addition of sodium butyrate, a histone deacetylase inhibitor. These findings demonstrate an approach to reactivate the function of genetic circuits in pluripotent stem cells to ensure robust function over many generations. Altogether, this work introduces an approach to overcome the silencing of genetic circuits in pluripotent stem cells that may enable the use of genetic circuits in pluripotent stem cells for long-term function.

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Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications

Cited by 17 publications

References 245 publications

Learning from BMPs and their biophysical extracellular matrix microenvironment for biomaterial design

Learning from BMPs and their biophysical extracellular matrix microenvironment for biomaterial design

Synthetic Biology and Tissue Engineering: Toward Fabrication of Complex and Smart Cellular Constructs

Rosa26 docking sites for investigating genetic circuit silencing in stem cells

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