Bottom-up assembly of viral replication cycles

Staufer, Oskar; Gantner, Gösta; Platzman, Ilia; Tanner, Klaus; Berger, Imre; Spatz, Joachim P.

doi:10.1038/s41467-022-33661-7

Cited by 4 publications

(6 citation statements)

References 81 publications

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“…While plant viruses could prove to be more amenable as agents of design under the ELM concept, they lack the inherent morphogenic capacities we seek. This lack of native morphogenic effect, places them into a bottom-up category of engineering agents, as any desired effect would have to be synthetical transformed into the virus (Staufer et al 2022;Tian et al 2019). While a bottom-up approach has to add completely new functionality to the organisms, the contrasting top-down attempts to strip out information to a minimal desired function.…”

Section: Agent Pathogenmentioning

confidence: 99%

Polymorphic Plant Architectures: A Pathological Approach to Growing a Building

Gennett

2024

Preprint

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Considering the prospects of Engineered Living Materials (ELMs), a parallel can be drawn between a building and a tree. How we might grow a living building follows nearly the same processes as how we grow a healthy productive forest. Agential systems such as plants and fungi must respond to and navigate in their environment, naturally resulting in the polymorphic growth of architecture defined by the local stimuli. A symbiotic relationship between two systems provides a ready means to shape the natural plasticity during plant growth using pathogenic agents capable of inducing morphogenesis. Exotic infection morphologies from pathogens are examined from the emerging perspective of a growing building. Transitioning from forest pathogen to biodesign agent of a living botanical building, host-pathogen symbiosis intrinsically links the growth and management of both. How plant pathogens maybe employed in the growth of a building is uniquely transferable across research disciplines from forest to building.

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Section: Agent Pathogenmentioning

confidence: 99%

Polymorphic Plant Architectures: A Pathological Approach to Growing a Building

Gennett

2024

Preprint

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“…Viral nucleic acid is vital for assessing virus replication and disease infectivity. [ 5 ] Obviously, it is crucial to inactivate ARGs and viral nucleic acids while killing microbes.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Yao,

Luo,

Lin

et al. 2024

Advanced Science

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The escalating spread of drug‐resistant bacteria and viruses is a grave concern for global health. Nucleic acids dominate the drug‐resistance and transmission of pathogenic microbes. Here, imidazolium‐type poly(ionic liquid)/porphyrin (PIL‐P) based electrospun nanofibrous membrane and its cerium (IV) ion complex (PIL‐P‐Ce) are developed. The obtained PIL‐P‐Ce membrane exhibits high and stable efficiency in eradicating various microorganisms (bacteria, fungi, and viruses) and decomposing microbial antibiotic resistance genes and viral nucleic acids under light. The nuclease‐mimetic and photocatalytic mechanisms of the PIL‐P‐Ce are elucidated. Co‐infection wound models in mice with methicillin‐resistant S. aureus and hepatitis B virus demonstrate that PIL‐P‐Ce integrate the triple effects of cationic polymer, photocatalysis, and nuclease‐mimetic activities. As revealed by proteomic analysis, PIL‐P‐Ce shows minimal phototoxicity to normal tissues. Hence, PIL‐P‐Ce has potential as a “green” wound dressing to curb the spread of drug‐resistant bacteria and viruses in clinical settings.

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“…In this study, we take advantage of the tools provided by bottom-up synthetic biology for the controlled formation of SARS-CoV-2 viruses. 16 We assembled synthetic SARS-CoV-2 MiniVs to study the change in the affinity of the Omicron and Alpha S variants for ACE2 and lipid membranes. Utilizing small unilamellar vesicles (SUVs) with a precise lipid composition� mimicking that of the natural SARS-CoV-2�as a backbone for functionalization with the S variant of interest, we formed synthetic MiniVs of use under BSL-1 conditions.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we take advantage of the tools provided by bottom-up synthetic biology for the controlled formation of SARS-CoV-2 viruses . We assembled synthetic SARS-CoV-2 MiniVs to study the change in the affinity of the Omicron and Alpha S variants for ACE2 and lipid membranes.…”

Section: Introductionmentioning

confidence: 99%

Bottom-up Assembled Synthetic SARS-CoV-2 Miniviruses Reveal Lipid Membrane Affinity of Omicron Variant Spike Glycoprotein

Yagüe Relimpio,

Fink,

Bui

et al. 2023

ACS Nano

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The ongoing COVID-19 pandemic has been brought on by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The spike glycoprotein (S), which decorates the viral envelope forming a corona, is responsible for the binding to the angiotensin-converting enzyme 2 (ACE2) receptor and initiating the infection. In comparison to previous variants, Omicron S presents additional binding sites as well as a more positive surface charge. These changes hint at additional molecular targets for interactions between virus and cell, such as the cell membrane or proteoglycans on the cell surface. Herein, bottom-up assembled synthetic SARS-CoV-2 miniviruses (MiniVs), with a lipid composition similar to that of infectious particles, are implemented to study and compare the binding properties of Omicron and Alpha variants. Toward this end, a systematic functional screening is performed to study the binding ability of Omicron and Alpha S proteins to ACE2-functionalized and nonfunctionalized planar supported lipid bilayers. Moreover, giant unilamellar vesicles are used as a cell membrane model to perform competitive interaction assays of the two variants. Finally, two cell lines with and without presentation of the ACE2 receptor are used to confirm the binding properties of the Omicron and Alpha MiniVs to the cellular membrane. Altogether, the results reveal a significantly higher affinity of Omicron S toward both the lipid membrane and ACE2 receptor. The research presented here highlights the advantages of creating and using bottom-up assembled SARS-CoV-2 viruses to understand the impact of changes in the affinity of S for ACE2 in infection studies.

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Bottom-up assembly of viral replication cycles

Cited by 4 publications

References 81 publications

Polymorphic Plant Architectures: A Pathological Approach to Growing a Building

Polymorphic Plant Architectures: A Pathological Approach to Growing a Building

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Bottom-up Assembled Synthetic SARS-CoV-2 Miniviruses Reveal Lipid Membrane Affinity of Omicron Variant Spike Glycoprotein

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