Bottom‐Up Design of a Grafted Organic Selenide Interface for Sensitive Electrocatalytic Detection of Peroxynitrite

Abstract

Peroxynitrite is a reactive species that emerged as a major cytotoxic agent implicated in a host of pathophysiological conditions. Reports emphasized deleterious physiological reactivity of peroxynitrite with various cell components. Accurate determination of peroxynitrite concentration is inherently difficult. Detection methods based on electrochemistry are relatively limited and the pursuit of viable electrochemical probes or catalytic materials as interfaces for efficient peroxynitrite detection is still underway. We prepared and characterized a functional thin‐film material based on an organic selenide (4,4’ diaminodiphenyl selenide) grafted on graphite electrodes and used the interface in sensitive electrochemical determination of peroxynitrite. We characterized the grafted film material on the graphite interface using various physicochemical methods. We show that peroxynitrite is electrocatalytically oxidized on the grafted selenide film. The grafted functional film material showed a significant electrocatalytic enhancement in the presence of peroxynitrite compared to controls. The selenide‐based peroxynitrite sensors show a sensitivity of 200 nA/μM and a lower limit of detection of 250 nM. To the best of our knowledge, this is the first time a grafted selenium‐based material on an electrode is used for catalytic detection and quantification of peroxynitrite.

Multi-engineered Graphene Extended-Gate Field-Effect Transistor for Peroxynitrite Sensing in Alzheimer’s Disease

“…Nevertheless, it is very hard to quantify ONOO – due to its high activity in a complex environment. Fluorescent probes, electrochemical, immunohistochemistry, or chemiluminescence methods have been reported to detect ONOO – , but high-performance assays are still relatively limited. − It is thus highly desirable to develop cost-effective and high-performance sensors for monitoring ONOO – toward the early diagnosis of AD.…”