Bovine-associated staphylococci and mammaliicocci trigger T-lymphocyte proliferative response and cytokine production differently

Souza, Fernando Nogueira de; Santos, Karina Cecília Panelli; Ferronatto, José Augusto; Sánchez, E.; Silva, Bruno Toledo; Heinemann, Marcos Bryan; Vliegher, Sarne De; Libera, Alice Maria Melville Paiva Della

doi:10.3168/jds.2022-22529

Cited by 10 publications

(5 citation statements)

References 54 publications

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“…Staphylococcus aureus can express a wide array of secreted virulence factors, which can interact with innate and adaptive immune responses, influencing leukocyte activation [ 24 , 25 ], and the particular virulence pattern of each strain is strongly related to the S. aureus genotype [ 8 , 12 , 14 , 26 ]. Recently, a comparative secretome study has analyzed the secreted protein profile of GTB/ST8 and GTS/ST398 strains [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, T cell expansion is promoted by IL-1β toward Th1, Th2, and Th17 [ 40 – 42 ]. Souza et al [ 24 ] demonstrated that bovine PBMC stimulated with different S. aureus strains causing persistent IMI increased IL-17 A and IFN-γ release in the supernatant, while only S. aureus strain promoted the lymphocyte polarization toward CD4 + and CD8 + phenotypes. The present work demonstrated that the challenge with 2.5% secretome increased the expression of IL-1β after 18 h and the PBMC viability after 48 h, supporting the hypothesis that S. aureus GTB/ST8 secreted molecules may induce lymphocyte activation and proliferation in vitro, also triggering an adaptive immune response.…”

Section: Discussionmentioning

confidence: 99%

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The secretome of Staphylococcus aureus strains with opposite within-herd epidemiological behavior affects bovine mononuclear cell response

Di Mauro,

Filipe,

Facchin

et al. 2023

Vet Res

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Staphylococcus aureus modulates the host immune response directly by interacting with the immune cells or indirectly by secreting molecules (secretome). Relevant differences in virulence mechanisms have been reported for the secretome produced by different S. aureus strains. The present study investigated the S. aureus secretome impact on peripheral bovine mononuclear cells (PBMCs) by comparing two S. aureus strains with opposite epidemiological behavior, the genotype B (GTB)/sequence type (ST) 8, associated with a high within-herd prevalence, and GTS/ST398, associated with a low within-herd prevalence. PBMCs were incubated with different concentrations (0%, 0.5%, 1%, and 2.5%) of GTB/ST8 and GTS/ST398 secretome for 18 and 48 h, and the viability was assessed. The mRNA levels of pro- (IL1-β and STAT1) and anti-inflammatory (IL-10, STAT6, and TGF-β) genes, and the amount of pro- (miR-155-5p and miR-125b-5p) and anti-inflammatory (miR-146a and miR-145) miRNAs were quantified by RT-qPCR. Results showed that incubation with 2.5% of GTB/ST8 secretome increased the viability of cells. In contrast, incubation with the GTS/ST398 secretome strongly decreased cell viability, preventing any further assays. The GTB/ST8 secretome promoted PBMC polarization towards the pro-inflammatory phenotype inducing the overexpression of IL1-β, STAT1 and miR-155-5p, while the expression of genes involved in the anti-inflammatory response was not affected. In conclusion, the challenge of PBMC to the GTS/ST398 secretome strongly impaired cell viability, while exposure to the GTB/ST8 secretome increased cell viability and enhanced a pro-inflammatory response, further highlighting the different effects exerted on host cells by S. aureus strains with epidemiologically divergent behaviors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The secretome of Staphylococcus aureus strains with opposite within-herd epidemiological behavior affects bovine mononuclear cell response

Di Mauro,

Filipe,

Facchin

et al. 2023

Vet Res

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“…Transcripts and protein level evaluations of expression of proinflammatory chemokines and cytokines in the udder of cows with chronic mastitis due to coagulase-positive and coagulase-negative Staphylococci showed no difference between the Staphylococci ( 128 ). In another study, S. aureus was known to cause persistent intramammary infection-induced proliferation of CD4+ and CD8+ lymphocytes, whereas S. aureus (originated from nostrils) and S. chromogenes strains (known to cause persistent IMI) had no effect on T and B cell proliferation ( 129 ). The authors showed that both S. aureus and S. chromogenes originating from persistent IMI significantly increased IL-17A and IFN-γ production from peripheral blood mononuclear cells.…”

Section: Bovine Staphylococcal Mastitismentioning

confidence: 99%

Staphylococcal mastitis in dairy cows

Kerro Dego,

Vidlund

2024

Front. Vet. Sci.

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Bovine mastitis is one of the most common diseases of dairy cattle. Even though different infectious microorganisms and mechanical injury can cause mastitis, bacteria are the most common cause of mastitis in dairy cows. Staphylococci, streptococci, and coliforms are the most frequently diagnosed etiological agents of mastitis in dairy cows. Staphylococci that cause mastitis are broadly divided into Staphylococcus aureus and non-aureus staphylococci (NAS). NAS is mainly comprised of coagulase-negative Staphylococcus species (CNS) and some coagulase-positive and coagulase-variable staphylococci. Current staphylococcal mastitis control measures are ineffective, and dependence on antimicrobial drugs is not sustainable because of the low cure rate with antimicrobial treatment and the development of resistance. Non-antimicrobial effective and sustainable control tools are critically needed. This review describes the current status of S. aureus and NAS mastitis in dairy cows and flags areas of knowledge gaps.

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“…In the last years, the epidemiology of NASM species and their relationships with bovine mammary gland health are getting increasing attention (Lienen et al 2022; Ruiz-Romero and Vargas-Bello-Pérez 2023). Studies have shown that different NASM behave differently, which can lead to different outcomes in mastitis cases (De Buck et al 2021;Souza et al 2023). Therefore, obtaining a precise species identi cation can be relevant; by clarifying the relationships between species and clinical outcomes, we might be able to ultimately improve animal health (Condas et al 2017; Ruiz-Romero and Vargas-Bello-Pérez 2023).…”

Section: Introductionmentioning

confidence: 99%

An update on non-aureus staphylococci and mammaliicocci in cow milk: unveiling the presence of Staphylococcus borealis and Staphylococcus rostri by MALDI-TOF MS

Penati,

Ulloa,

Locatelli

et al. 2024

Preprint

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Non-aureus staphylococci and mammaliicocci (NASM) are microorganisms most frequently isolated from milk. Given their numerosity and complexity, MALDI-TOF MS is one of the preferred species identification approaches. Nevertheless, reference mass spectra for the novel species Staphylococcus borealis were included only recently in the Bruker Biotyper System (MBT) library, and other species of veterinary interest such as S. rostri are still absent. This work provides an updated picture of the NASM species found in milk, gained by retrospectively analyzing the data relating to 21,864 milk samples, of which 6,278 from clinical mastitis (CM), 4,039 from subclinical mastitis (SCM), and 11,547 from herd survey (HS), with a spectrum library including both species. As a result, S. borealis was the second most frequently isolated NASM (17.07%) after S. chromogenes (39.38%) in all sample types, with a slightly higher percentage in CM (21.84%), followed by SCM (17.65%), and HS (14.38%). S. rostri was also present in all sample types (3.34%), reaching 8.43% of all NASM in SCM and showing a significant association (p < 0.01) with this condition. Based on our findings, the presence of S. borealis and S. rostri in milk and their potential association with mastitis has been overlooked, possibly due to the difficulties in differentiating these species from other closely related NASM. Our results indicate that S. borealis might be a more frequent contributor to bovine udder infections than previously thought and that S. rostri should also not be underestimated considering its significant association with SCM.

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Bovine-associated staphylococci and mammaliicocci trigger T-lymphocyte proliferative response and cytokine production differently

Cited by 10 publications

References 54 publications

The secretome of Staphylococcus aureus strains with opposite within-herd epidemiological behavior affects bovine mononuclear cell response

The secretome of Staphylococcus aureus strains with opposite within-herd epidemiological behavior affects bovine mononuclear cell response

Staphylococcal mastitis in dairy cows

An update on non-aureus staphylococci and mammaliicocci in cow milk: unveiling the presence of Staphylococcus borealis and Staphylococcus rostri by MALDI-TOF MS

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