Bovine Babesiosis and Anaplasmosis in some Cattle Farms in the Vina Division

Abdoulmoumini, Mamoudou; Cyril, Nguetoum; Lendzele, Sevidzem Silas; Kingsley, Manchang Tanyi; Njongui, Jean Ebene; Pagnah, Zoli Andre

doi:10.5455/ijlr.20170423025510

Cited by 6 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anaplasma marginale and A. centrale are gram-negative bacteria of the order Rickettsiales, and known to cause bovine anaplasmosis in tropical and subtropical regions [6]. The prevalence in the present study ( A. marginale : 21.9%, A. centrale : 7.8%) was significantly lower than reported in a recent study from North Cameroon with 62.2% and 53.3%, respectively [7], using Giemsa staining. Conversely, our results were higher than reported in the North-West region where the prevalence was 2.2% for A. marginale and 0% for A. centrale , respectively [6].…”

Section: Discussioncontrasting

confidence: 73%

“…Two groups of genotypes were found, one of which had yet no listed entry in GenBank (GenBank: MN120882). The absence of detection of this pathogen in previous studies from Cameroon is very likely due to its misidentification for other TBPs [7]. Furthermore, the clade A. platys matched very well with Anaplasma sp.…”

Section: Discussionmentioning

confidence: 87%

“…The prevalence of the various TBPs and their interdependences in Cameroon are not well investigated. Most of the studies used conventional microscopy of blood-smears, serology, or post-mortem analyses [6, 7] which all have considerable limitations. Identification of individual species of pathogens is almost impossible without the intervention of molecular tools, like PCR.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular identification and prevalence of tick-borne pathogens in zebu and taurine cattle in North Cameroon

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background Public interest for tick-borne pathogens in cattle livestock is rising due to their veterinary and zoonotic importance. Consequently, correct identification of these potential pathogens is crucial to estimate the level of exposition, the risk and the detrimental impact on livestock and the human population. Results Conventional PCR with generic primers was used to identify groups of tick-borne pathogens in cattle breeds from northern Cameroon. The overall prevalence in 1260 blood samples was 89.1%, with 993 (78.8%) positive for Theileria/Babesia spp., 959 (76.1%) for Anaplasma/Ehrlichia spp., 225 (17.9%) for Borrelia spp., and 180 (14.3%) for Rickettsia spp. Sanger sequencing of a subset of positively-tested samples revealed the presence of Theileria mutans (92.2%, 130/141), T. velifera (16.3%, 23/141), Anaplasma centrale (10.9%, 15/137), A. marginale (30.7%, 42/137), A. platys (51.1%, 70/137), Anaplasma sp. ‘Hadesa’ (10.9%, 15/137), Ehrlichia ruminantium (0.7%, 1/137), E. canis (0.7%, 1/137), Borrelia theileri (91.3%, 42/46), Rickettsia africae (59.4%, 19/32) and R. felis (12.5%, 4/32). A high level of both intra- and inter-generic co-infections (76.0%) was observed. To the best of our knowledge, B. theileri, T. mutans, T. velifera, A. platys, Anaplasma sp. ‘Hadesa’, R. felis and E. canis are reported for the first time in cattle from Cameroon, and for R. felis it is the first discovery in the cattle host. Babesia spp. were not detected by sequencing. The highest number of still identifiable species co-infections was up to four pathogens per genus group. Multifactorial analyses revealed a significant association of infection with Borrelia theileri and anemia. Whereas animals of older age had a higher risk of infection, the Gudali cattle had a lower risk compared to the other local breeds. Conclusion Co-infections of tick-borne pathogens with an overall high prevalence were found in all five study sites, and were more likely to occur than single infections. Fulani, Namchi and Kapsiki were the most infected breed in general; however, with regions as significant risk factor. A better-adapted approach for tick-borne pathogen identification in co-infected samples is a requirement for epidemiological investigations and tailored control measures.

show abstract

Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular identification and prevalence of tick-borne pathogens in zebu and taurine cattle in North Cameroon

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…may not be detectable by molecular tools as the pathogen can be completely cleared from the blood stream and even from organs [24]. The evidence of Babesia in a study from Northern Cameroon [2] could indicate current or very recent infection event in the sampled individuals, allowing its identification on Giemsa stained blood smears.…”

Section: Discussionmentioning

confidence: 99%

“…For cattle and other livestock, the published literature highlights the importance of protozoa of the genera Babesia and Theileria , bacteria of the genera Anaplasma , Ehrlichia and Rickettsia , and arboviruses as etiologic agents of many diseases, of which a number of them have zoonotic potential [1]. Especially in developing countries, routine diagnostic approaches for the identification of TBPs are generally based on microscopic examination of blood smears [2,3] or serological assays [4,5]. While those techniques require only moderate investments for equipment and infrastructure, they have limitations regarding specificity and sensitivity (microscopy) [6,7,8], or tend to cross-react with closely related species (enzyme-linked immunosorbent assays) [9].…”

Section: Introductionmentioning

confidence: 99%

Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle

Abanda

Paguem

Achukwi³

et al. 2019

TropicalMed

View full text Add to dashboard Cite

In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA microarray kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset.

show abstract

Ticks (Acari: Ixodidae) and tick-borne diseases in Cameroon: Current understanding and future directions for more comprehensive surveillance

Ngnindji-Youdje,

Lontsi-Demano,

Diarra

et al. 2025

One Health

View full text Add to dashboard Cite

Bovine Babesiosis and Anaplasmosis in some Cattle Farms in the Vina Division

Abstract: Cattle infection with Babesiosis and Anaplasmosis like other Tick Borne Diseases (TBDs) is common in the Vina

Cited by 6 publications

References 3 publications

Molecular identification and prevalence of tick-borne pathogens in zebu and taurine cattle in North Cameroon

Molecular identification and prevalence of tick-borne pathogens in zebu and taurine cattle in North Cameroon

Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle

Ticks (Acari: Ixodidae) and tick-borne diseases in Cameroon: Current understanding and future directions for more comprehensive surveillance

Contact Info

Product

Resources

About