Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis

Tucker, Nisha; Cunha, Patricia; Gilbert, Florence; Rambault, Marion; Santos, Karina Cecília Panelli; Rémot, Aude; Germon, Pierre; Rainard, Pascal; Martins, Rodrigo Prado

doi:10.1016/j.jri.2023.103826

Cited by 3 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A subset CD3 + CD4 − CD8 − was evident with Panel 1 and Panel 2, according to the phenotype and percentage, which showed that these could have been γδT cells [ 42 ]. γδT cells represent between 15 and 60% of the circulating lymphocytes in the bovine system and are known as innate-like cells [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Application of Multiparametric Flow Cytometry Panels to Study Lymphocyte Subpopulations in Tuberculin-Positive Cattle

Manzo-Sandoval

Jaramillo-Meza

Olguín‐Alor

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

Flow cytometry (FC) is widely used in microbiology, immunology, hematology, and oncology. In the veterinary field, FC enabled the study of the immune response in cattle infected with different pathogens, as well as vaccine testing. However, few fluorochrome-conjugated antibodies recognize bovine antigens, limiting the possible benefits of FC and the implementation of multiparametric analysis for more complex studies. Two cytometry panels with five colors each were designed and implemented for the study and identification of populations and subpopulations of T cells derived from the peripheral blood mononuclear cells of dairy heifers. Both panels detected differences in T cell subpopulations between heifers positively and negatively tested for tuberculin; they detected overexpression of CD25+ and CD45RO+ in tuberculin-positive heifers after stimulation with a culture filtrate protein extract (CFPE) from Mycobacterium bovis (M. bovis). We identified subpopulations of T cells from peripheral blood mononuclear cells using two multicolor panels. These panels could be used to analyze total bovine blood in immunopathogenic studies and vaccine development. The same strategy could be implemented in other species of veterinary interest.

show abstract

Section: Discussionmentioning

confidence: 99%

Application of Multiparametric Flow Cytometry Panels to Study Lymphocyte Subpopulations in Tuberculin-Positive Cattle

Manzo-Sandoval

Jaramillo-Meza

Olguín‐Alor

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Unlike peripheral blood, T cells in healthy mammary glands and milk are mostly CD8 + (Sordillo & Streicher, 2002). However, increased amounts of CD4 + T cells concurrent with a decrease of their CD8 + counterparts is observed in milk during mastitis (Riollet et al, 2001; Soltys & Quinn, 1999; Tucker et al, 2023) indicating that these cells are important mediators of MG immune defences. Nevertheless, the contribution of CD8 + T cells to the protection of mammary tissue should not be neglected.…”

Section: Response To Infectious Agentsmentioning

confidence: 99%

Immune defences of the mammary gland in dairy ruminants

Germon

Martins

2023

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

The mammary gland (MG) of ruminants is essential for assuring the immune protection and nutrition of the suckling youngs. The domestication of these species aimed at increasing milk production for human consumption enhanced udder susceptibility to infections and in this context, a better understanding of the MG immune defences has become a cornerstone for the success of dairy farming. In this review, we explore constitutive and inducible immune mechanisms of the mammary gland and briefly discuss the knowledge gaps that remain to be elucidated for the implementation of strategies focused on boosting mammary immune responses.

show abstract