Bovine cardiac troponin-I specific monoclonal antibodies which show species cross reactivity

Haider, Kh. H.; Stimson, W. H.

doi:10.1038/emm.1996.11

Exp Mol Med

1996

DOI: 10.1038/emm.1996.11

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Bovine cardiac troponin-I specific monoclonal antibodies which show species cross reactivity

Kh. H. Haider

W. H. Stimson²

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1998

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Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model

Haider

Stimson

1998

Arch. Pharm. Res.

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The present study was carried out to determine the possible use of cTn-I in the cardiac myofibrillar architecture, as a potential target for in vivo radioimmunodetection of cardiac damage in a brain death pig model. Radioiodination of the anti-cTn-I 5F4 McAb was carried out by lactoperoxidase method. The percentage iodine incorporation achieved was 70-75%. The radioiodinated McAbs were purified on Sephadex G-25 column and characterised by Paper chromatography, Phast Gel electrophoresis and electroimmunoblotting. Radioiodinated anti-cTn-I 5F4 McAbs were employed alongside Pyrophosphate (Tc99m-PPi) and Thallium201 chloride (Tl201) in 24 landrace pigs (brain-dead = 18 & sham-operated = 6). The percentage cardiac uptake of the radiolabelled antibody injected dose was significantly higher in the brain dead animals (0.196%) as compared to that of sham-operated animals (0.11%). Specific in vivo localization of radiolabelled McAbs in the infarcted cardiac tissue was confirmed by computer-aided reconstruction of 3-D images of the isolated heart. The preliminary results of the study revealed preferential uptake of radiolabelled antibody at the site of myocyte damage resulting from artificially induced brain death.

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Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model

Haider

Stimson

1998

Arch. Pharm. Res.

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Bovine cardiac troponin-I specific monoclonal antibodies which show species cross reactivity

Cited by 1 publication

References 1 publication

Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model

Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model

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