Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling

García, Elina V.; Hamdi, Meriem; Barrera, Daniel; Sánchez‐Calabuig, María Jesús; Gutiérrez‐Adán, Alfonso; Rizos, Dimitrios

doi:10.1530/rep-16-0654

Cited by 35 publications

(31 citation statements)

References 62 publications

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“…The hypothesis that the presence of an embryo is able to elicit changes in the oviduct epithelium transcriptome is based on findings from mammals such as mice (Lee et al, 2002), swine (Almiñana et al, 2012) and horses (Smits et al, 2016). Furthermore, in support of the hypothesis that the embryo is able to communicate with the oviduct, in vitro studies have shown that bovine oviduct epithelial cell (BOEC) transcriptome is affected by the embryo presence (García et al, 2017;Schmaltz-Panneau et al, 2014). In cattle, while the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes (Maillo et al, 2015), attempts to detect the effect of a single bovine embryo on the oviductal isthmus were unsuccessful (Maillo et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Then, the cycle threshold (CT) or the cycle during the log‐linear phase of the reaction at which fluorescence increased above background was determined for each sample. The ΔCT value was determined by subtracting the endogenous control ( ACTB; reference gene previously tested in oviduct epithelial cells in in vivo (Maillo et al, ) and in vitro (García et al, ) studies) C T value for each sample from each gene CT value of the sample. Calculation of ΔΔ C T involved using the highest sample Δ C T value (i.e., the sample with the lowest target expression) as a constant to subtract from all other Δ C T sample values.…”

Section: Methodsmentioning

confidence: 99%

“…Then, the cycle threshold (CT) or the cycle during the log-linear phase of the reaction at which fluorescence increased above background was determined for each sample. The ΔCT value was determined by subtracting the endogenous control (ACTB; reference gene previously tested in oviduct epithelial cells in in vivo (Maillo et al, 2015) and in vitro (García et al, 2017)…”

Section: Rna Extraction Reverse Transcription and Quantitative Reamentioning

confidence: 99%

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An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

Rodríguez‐Alonso

Hamdi²,

Sánchez

et al. 2019

Reprod Domestic Animals

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This study aimed to examine the local embryo effect on the transcriptomic response of the epithelial cells of the oviduct in vivo. Fifteen heifers were synchronized and artificially inseminated to a standing heat. All heifers were slaughtered on Day 2.5 after oestrus. The oviducts from 13 animals were isolated, trimmed free of tissue and divided between ampulla/isthmus. The ipsilateral isthmus was divided into smaller sections (2 cm). Each section was sequentially flushed until the embryo was located (4/13) and then opened and scraped longitudinally to obtain the epithelial cells. Cells were snap‐frozen in LN2 for gene expression analysis. All recovered embryos were found at the beginning of the isthmus. The 2 cm sections selected for the transcriptomic analysis were as follows: embryo section (in which the embryo was found); proximal section (through which the embryo had passed); distal section (on the uterine side of the embryo); and contralateral section (section from the contralateral isthmus). The expression pattern of eight genes (STK32A, KERA, QRFPR, MCTP1, PRELP, VAT1L, SOCS3 and CCL20) differentially expressed between the isthmus of pregnant (multiple embryo model) and cyclic heifers were assessed by RT‐qPCR. One‐way ANOVA and t test was used for statistical analysis. Comparisons between ipsilateral and contralateral oviduct or along the ipsilateral oviduct resulted in no differences for all genes. Despite the failure to detect a site‐specific response of a single embryo on the abundance of distinct transcripts in the bovine oviduct in vivo on Day 2.5, the current methodology with proposed modifications would be useful for future studies to examine the local embryo effect.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Rna Extraction Reverse Transcription and Quantitative Reamentioning

confidence: 99%

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An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

Rodríguez‐Alonso

Hamdi²,

Sánchez

et al. 2019

Reprod Domestic Animals

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“…Differences between the ipsi-and contralateral sides were observed. García et al (2017) used an in vitro model to correlate processes in bovine oviduct epithelial cells with the developmental stage of the embryos. For this, Fallopian epithelial cells were cultured in vitro and covered with a woven polyester mesh having a grid size of 41 × 41 openings.…”

Section: Exchange Of Signals Between Embryo and Oviduct In Cattlementioning

confidence: 99%

Review: Environmental impact on early embryonic development in the bovine species

Besenfelder

Brem

Havlíček

2020

Animal

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Assisted reproduction techniques (ARTs) provide access to early stage embryos whose analysis and assessment deliver valuable information. The handling of embryos, including the in vitro production of bovine embryos, is a rapidly evolving area which nonetheless exposes the embryos to unnatural conditions for a period of time. The Fallopian tube provides innumerable quantitative and qualitative factors, all of which guarantee the successful development of the embryo. It is well known that the Fallopian tube can be bypassed, using embryo transfer, resulting in successful implantation in the target recipient animal and the birth of calves. However, the question arises as to whether such circumvention has a negative impact on the embryo during this sensitive development period. First crosstalk between the embryo and its environment confirms mutual recognition activities and indicate bilateral effects. Nowadays, in vitro production of bovine embryos is a well-established technology. However, it is still evident that in vitro generated embryos are not qualitatively comparable to embryos obtained ex vivo. To counteract these differences, comparative studies between in vitro and ex vivo embryos are advantageous, as embryos grown in their physiological environment can provide a blueprint or gold standard against which to compare embryos produced in vitro. Attempts to harness the bovine oviduct were sometimes very invasive and did not result in wide acceptance and routine use. Long-term development and refinement of transvaginal endoscopy for accessing the bovine oviduct has meanwhile been routinely applied for research as well as in practice. Comparative studies combining in vitro development with development in the cattle oviduct revealed that the environmental conditions to which the embryo is exposed before activation of the embryonic genome can have detrimental and lasting effects on its further development. These effects are manifested as deviations in gene expression profiles and methylation signatures as well as frequency of whole chromosomal or segmental aberrations. Furthermore, it was shown that hormonal superstimulation (multiple ovulation and embryo transfer), varying progesterone concentrations as well as metabolic disorders caused by high milk production, markedly affected embryo development in the postpartum period. Assisted reproductive techniques that allow the production and handling of extra numbers of generated embryos promise to have a very high impact on scientific and practical application. Any influence on the early embryonic life, both in animals and in vitro, is accompanied by a sensitive change in embryonic activity and should be assessed in vivo on the basis of physiological conditions before being used for ART.

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“…In several studies bovine embryos were cocultured with OEC to mimic the maternal environment in vitro. These experiments demonstrated bi-directional responses at the transcriptional level from both the maternal and embryo side (Schmaltz-Panneau et al, 2014;Garcia et al, 2017). However, OEC were subjected to multiple embryos and/or prolonged coincubation, which does not ideally imitate the in vivo situation in the monovulatory cow.…”

Section: Co-culture Of Embryos With Epithelia Of the Frtmentioning

confidence: 99%

Modeling early embryo-maternal interactions in vitro

Schoen¹,

Chen²

2018

Anim Reprod

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Environmental conditions experienced during early embryonic development influence growth, metabolism, and gene expression of the embryo as well as the epigenetic profile of the offspring. The environment of the early embryo consists of the luminal fluid within the oviduct and uterus and the epithelial cells composing this fluid. Whether the embryo is able to shape its own microenvironment by interacting with the epithelial lining of the oviduct/uterus and which factors potentially interfere with or regulate these interactions remains to be elucidated. As early embryonic signals and the respective maternal responses are subtle and local events, it is challenging to study them in vivo. Therefore, adequate in vitro-models optimally mimicking the contact zone between the maternal reproductive tract and the early embryo are needed to a) elucidate basic mechanisms involved in early embryonic development and b) reduce the number of experimental animals used for such studies. Functional epithelial cells are generally defined by a polarized distribution of organelles and proteins. Proper polarization is tightly connected with physiological cell behavior and in vivo-like reactivity of the epithelium. Therefore, this review summarizes current strategies for in vitro preservation of epithelial cell polarity. It presents recent advances in 3D culture of female reproductive tract epithelia and embryo-epithelial cocultures. A special emphasis is set on compartmentalized culture systems, powerful tools for studying early embryo-maternal interactions in vitro. In such systems, cultured epithelial cells are manipulable from their basolateral as well as their apical cell pole, allowing concomitant application of embryonic as well as maternal effectors from the appropriate cellular compartment.

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Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling

Cited by 35 publications

References 62 publications

An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

An approach to study the local embryo effect on gene expression in the bovine oviduct epithelium in vivo

Review: Environmental impact on early embryonic development in the bovine species

Modeling early embryo-maternal interactions in vitro

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