Bovine endometrial stromal cells display osteogenic properties

Donofrío, Gaetano; Franceschi, Valentina; Capocefalo, Antonio; Cavirani, S.; Sheldon, I. Martin

doi:10.1186/1477-7827-6-65

Cited by 48 publications

(53 citation statements)

References 38 publications

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“…The uterine cells differentiated in vitro, under proper conditions, into three mesenchymal lineages: adipogenic, chondrogenic, and osteogenic. These results suggest the wide plasticity of phenotype of bovine uterine cells and the presence of undifferentiated stem cells in the bovine uterus, thus we confirmed and extended the results obtained by Donofrio et al (2008).…”

Section: Discussionsupporting

confidence: 80%

“…To our knowledge, this is the first study that concerned the identification of stem cell markers in the bovine uterus relative to animals' age, uterine horn side, and type of uterine cells. Donofrio et al (2008) were the first ones to isolate from the bovine uterus a population of endometrial stromal cells that displayed the capacity to differentiate in vitro into bone tissue. That study suggests that in the bovine endometrium exists a population of cells possessing wide plasticity of phenotype, and that they may differentiate into distinct mesenchymal cell lineages, which are characteristics of MSc (Donofrio et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Donofrio et al (2008) were the first ones to isolate from the bovine uterus a population of endometrial stromal cells that displayed the capacity to differentiate in vitro into bone tissue. That study suggests that in the bovine endometrium exists a population of cells possessing wide plasticity of phenotype, and that they may differentiate into distinct mesenchymal cell lineages, which are characteristics of MSc (Donofrio et al 2008). In women, stem cells are present in uterine tissue throughout their whole life (Cho et al 2004).…”

Section: Discussionmentioning

confidence: 99%

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Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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“…Thus, there is great commonality between uterine-and bone-signaling pathways, and activation of SMO in the uterus leads to a chondrocytic phenotype. Furthermore, cultured endometrial stromal cells, both human and bovine, have been shown to exhibit chondrocytic/osteogenic properties, which may occur as a result of a perturbation in endometrial Hh signaling [43,44]. In addition to demonstrating that Hh activation results in the induction of genes involved in chondrocyte proliferation and differentiation, these data also suggest that SMO activation may be affecting a progenitor cell population as the uterine stromal cells exhibit an altered differentiation profile; additional studies need to be conducted to explore this further.…”

Section: Discussionmentioning

confidence: 99%

Constitutive Activation of Smoothened Leads to Female Infertility and Altered Uterine Differentiation in the Mouse1

Franco

Lee

Rubel

et al. 2010

Biology of Reproduction

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Previous work has identified Indian hedgehog (Ihh) as a major mediator of progesterone signaling during embryo implantation. Ihh acts through its downstream effector smoothened (Smo) to activate the GLI family of transcription factors. In order to gain a better understanding of Ihh action during embryo implantation, we expressed a Cre-recombinase-dependent constitutively activated SMO in the murine uterus using the Pgr(tm2(cre)Lyd) (PR(cre)) mouse model [Pgr(tm2(cre)Lyd+)Gt(ROSA)26Sor(tm1(Smo/EYFP)Amc)(+) (PR(cre/+)SmoM2(+))]. Female PR(cre/+)SmoM2(+) mice were infertile. They exhibited normal serum progesterone levels and normal ovulation, but their ova failed to be fertilized in vivo and their uterus failed to undergo the artificially induced decidual response. Examination of the PR(cre/+)SmoM2(+) uteri revealed numerous features such as uterine hypertrophy, the presence of a stratified luminal epithelial cell layer, a reduced number of uterine glands, and an endometrial stroma that had lost its normal morphologic characteristics. Microarray analysis of 3-mo-old PR(cre/+)SmoM2(+) uteri demonstrated a chondrocytic signature and confirmed that constitutive activation of PR(cre/+)SmoM2(+) increased extracellular matrix production. Thus, constitutive activation of Smo in the mouse uterus alters postnatal uterine differentiation which interferes with early pregnancy. These results provide new insight into the role of Hedgehog signaling during embryo implantation.

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“…58 Cabezas et al 59 studied the uteri of healthy Holstein cows at the beginning of the oestrous cycle luteal phase (days 1-5 post-ovulation) and during the luteal phase (between days 13 and 18 post-ovulation) to search for stem cells. These authors were able to identify the expression of markers for embryonic stem cells (OCT4 and SOX2) and MSCs (STAT3, CD44 and c-KIT) in the cow endometria during both periods of the oestrous cycle luteal phase.…”

Section: Endometrial Mscs In Cowsmentioning

confidence: 99%

The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine

2017

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Mesenchymal stem cells (MSCs) have been isolated from the endometrium of humans, mice, cows, pigs and ewes. Typically, these cells are detected in the deep regions of the endometrium, closer to the union with the myometrium. MSCs possess characteristics such as clonogenicity and multipotentiality since they can differentiate in vitro into adipogenic, chondrogenic and osteogenic lineages. These cells can be induced to differentiate in vitro not only into the mesodermal lineage but also into the endodermal and ectodermal lineages. Therefore, MSCs show a great regenerative capacity for various organs and tissues, including the endometrium. Some advantages of endometrial MSCs compared with other MSC sources are their immune modulating activity, their ease of obtainment, and the amount of sample that may be collected. The study of endometrial MSCs in domestic animals is a new and promising field because increasing our understanding of the physiology and biology of these cells may lead to a better understanding of the physiopathology of reproductive diseases, and the development of treatment methods for infertility problems. In other veterinary medicine fields, MSCs can be used for the treatment of autoimmune diseases, cardiac affections, musculoskeletal and articular lesions, muscle degeneration, type 1 diabetes, urinary tract diseases, neurodegenerative processes and tumours. Finally, MSCs are also an important clinical tool for tissue engineering and regenerative medicine. The aim of this review is to present an updated outlook of the knowledge regarding endometrial MSCs and their possible applications in veterinary medicine.

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Bovine endometrial stromal cells display osteogenic properties

Cited by 48 publications

References 38 publications

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Constitutive Activation of Smoothened Leads to Female Infertility and Altered Uterine Differentiation in the Mouse1

The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine

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