Bovine epididymal spermatozoa: Resistance to cryopreservation and binding ability to oviductal cells

Cunha, Andrielle Thainar Mendes; Carvalho, J. F.; Kussano, N. R.; Martins, Claudia Miranda; Mourão, Gérson Barreto; Dode, M. A. N.

doi:10.1016/j.cryobiol.2016.09.170

Cited by 10 publications

(10 citation statements)

References 55 publications

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“…The results showed that EP presented a higher resistance during incubation and maintained total motility, progressive motility, the percentage of cells with mitochondrial potential and stable membrane for a longer time than the EJ sperm. The higher stability of EP sperm post-thawing has been previously reported in other studies [8, 11, 20]. However, the exposure of EP sperm to heparin altered their resistance, and the sperm exhibited a pattern of motility and viability similar to that observed for the EJ sperm.…”

Section: Discussionsupporting

confidence: 78%

“…Sperm samples were collected from ejaculated and epididymal of the same animal, according to the method described by Cunha et al [8]. Briefly, one ejaculate was collected via electroejaculation from each Gir bull.…”

Section: Methodsmentioning

confidence: 99%

“…Studies have shown that EP is more resistant than EJ and presents greater viability after refrigeration and longevity after thawing [8]. Considering these results, it can be assumed that other aspects related to sperm functionality are also different between EP and EJ.…”

Section: Introductionmentioning

confidence: 99%

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Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

et al. 2019

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This study aimed to establish a protocol for in vitro embryo production using epididymal sperm (EP). Samples were obtained from ejaculated sperm (EJ) and the epididymis of 7 Gir bulls. First, the effect of heparin (+) on the viability, longevity (Experiment 1) and fertilization rates (Experiment 2) of the EP was evaluated. In experiment 2, a pool of EP and EJ sperm (n = 7) was coincubated with cumulus-oocyte complexes (COCs) for 0, 3, 6, 12 and 18 h, and the fertilization rate (FR) was evaluated. A third experiment was performed to test sperm treatments for IVP using the Percoll (P) or PureSperm (PS) gradients or a spTALP wash for sperm selection. Cleavage, blastocyst rate (BR) and embryo sex were evaluated. In experiment 4, embryos were produced using 6, 12, and 18 h of sperm-oocyte coincubation. The cleavage, BR, and total number and percentage of apoptotic cells were determined. Heparin affected EP viability, longevity and FR. After 6 h, 82% of the oocytes were fertilized in the EP+ group, a higher value (P<0.05) than that in the EJ (19%) and EP- (42%) groups. At 12 and 18 h, FR remained higher in the EP+ group, and a gradual increase in polyspermy was observed. The use of a P or PS gradient yielded a similar BR on D7 (54% and 52%), which was higher than the rate obtained using the washing method (37%). The embryos produced by EP and selected in a P or PS gradient resulted in a sex deviation in favor of male embryos (P>0.05). No differences (P>0.05) were observed among the groups that were coincubated for 6, 12 and 18 h with respect to embryo production, kinetics of development, total cell number and percentage of apoptotic cells. In conclusion, IVF time can be reduced to 6 h without affecting embryo production and quality. In addition, EP sperm selection can be performed by either a PS or P gradient.

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Section: Discussionsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

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Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

et al. 2019

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“…Motility is considered one of the most important factors for assessing sperm quality and functionality (Baghshahi et al, ). In this context, our results are in the average (32%–86%) found for spermatozoa of the post‐mortem epididymis of the bovine species (Cunha et al, ; Goovaerts et al, ; Nichi et al, ). In the present study, the incubation time had a significant effect on the membrane functional integrity, membrane structural integrity, mitochondrial activity, progressive motility and some kinetic parameters, as expected.…”

Section: Discussionsupporting

confidence: 62%

“…Thus, we consider EOSA15 an interesting antioxidant to use during sperm capacitation or pre‐treatment for IVF. Additionally, we chose the epididymal spermatozoa model due to its availability in slaughterhouses and a similar quality and resemblance resistance to ejaculated spermatozoa (Cunha et al, ). These spermatozoa are an alternative to maximise the reproductive potential of animals of economic interest, wild or threatened, affected by diseases that prevent ejaculate collection, or after unexpected death (Kaabi et al, ; Nichi et al, ).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of the essential oil ofSyzygium aromaticumon bovine epididymal spermatozoa

et al. 2019

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Focusing on its application in reproductive biotechnology, we evaluated the effects of the essential oil of Syzygium aromaticum (EOSA) on bovine epididymal sperm quality variables, including morphology, membrane functional integrity, membrane structural integrity, mitochondrial activity, metabolic activity, motility and oxidative stress by reactive oxygen species (ROS) levels. Bovine spermatozoa from eight males were incubated into the following groups: EOSA0 (without EOSA), EOSA10 (10 μg/ml of EOSA), EOSA15 (15 μg/ml of EOSA) and EOSA20 (20 μg/ml of EOSA); the incubation time with and without the EOSA was 1 or 6 hr. None of the sperm quality variables presented difference among the EOSA concentrations. However, the incubation time had a significant effect on the membrane functional integrity, membrane structural integrity, mitochondrial activity, progressive motility and some kinetic parameters. The effect of interaction among EOSA and incubation time was significant only on ROS levels. Spermatozoa incubated in the presence of 15 μg/ml of the EOSA for 1 hr had significantly reduced ROS levels compared with all other groups in the same time. In conclusion, the EOSA at a concentration of 15 µg/ml has antioxidant effects and protects bovine epididymal spermatozoa; hence, the EOSA may potentially be used in the field of reproductive biotechnology.

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Seminal cell–free DNA as a potential marker for in vitro fertility of Nellore bulls

Dode,

Capobianco,

Vargas

et al. 2024

J Assist Reprod Genet

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Bovine epididymal spermatozoa: Resistance to cryopreservation and binding ability to oviductal cells

Cited by 10 publications

References 55 publications

Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

Antioxidant effects of the essential oil ofSyzygium aromaticumon bovine epididymal spermatozoa

Seminal cell–free DNA as a potential marker for in vitro fertility of Nellore bulls

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