Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows

Oliveira, André Alves de; Heinemann, Marcos Bryan; Cortéz, Adriana; Nascimento, P. M. P.; Leite, Rômulo Cerqueira; Viana, J. H. M.

doi:10.1590/s0100-204x2016000500031

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Researchers using the polymerase chain reaction (PCR) test to detect BoHV-1 DNA have concluded that the risk of transmission of BoHV-1 by COCs and follicular fluid from seropositive cows without clinical symptomatology is irrelevant (Oliveira et al 2016), since the presence of viral DNA was not observed in any of their tested samples.…”

Section: Discussionmentioning

confidence: 99%

Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1)

et al. 2018

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Bovine herpesvirus 1 (BoHV-1) is an important bovine pathogen that is responsible for causing respiratory diseases and reproductive failures. The presence of BoHV-1 in an in vitro embryo production system affects fertilization, maturation, and embryonic development. The objective of this study was to evaluate the developmental capacity of oocytes from naturally infected cows with no reproductive history. Moreover, this study investigated the presence of viral DNA in cumulus oophorus complexes (COCs). Experimental groups were differentiated by titrating the antibodies detected through seroneutralization assays, establishing three groups: seronegative animals (titer lower than 2), low titer (2 to 8), and animals with a titer above or equal to 16. COCs were obtained from 15 donors during 22 sessions of ultrasound-guided follicular aspiration. DNA was extracted from a pool of COCs obtained from all aspirations from the same donor as well as from whole blood and nested PCR reactions were performed. Only COCs with a compact layer of cumulus cells, an intact zona pellucida, and homogeneous cytoplasm were selected for in vitro culture and evaluation of nuclear maturation rate. After culturing for 24 hours, the oocytes were fixed and stained to evaluate the meiotic cell cycle stage. Oocytes that showed a chromosomal configuration in metaphase II were considered to have reached nuclear maturation. Compared with the other groups, the oocyte nuclear maturation rate in animals with a titer greater than or equal to 16 (50%) was compromised (P< 0.05). However, the viral titer did not influence the maturation rate of bovine oocytes in animals exhibiting low titration (62.2%) when compared with the control group (76.7%). Viral DNA was not observed in the blood samples but was detected in the COC pool from three seropositive donors. In view of the results obtained, we conclude that natural infections by the BoHV-1 virus can compromise the nuclear maturation rate in cows, depending on the titration levels of antibodies against the virus. Moreover, viral DNA could be present in COCs, contradicting the hypothesis that seropositive animals with no history of clinical symptomatology pose a negligible risk of transmitting BoHV-1 by COCs.

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Section: Discussionmentioning

confidence: 99%

Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1)

et al. 2018

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“…However, the effectiveness of trypsin washing for decontamination of in vitro-produced bovine embryos is controversial. 16 In support of this contention, while several studies demonstrated that trypsin treatment is very effective for the removal or inactivation of BoHV-1, 7,10,17 other investigators found that the inclusion of trypsin solution in the washing procedure may be sufficient to strip BoHV-1 from the surface of the embryo but has no such effect on virus particles once inside. 14,18,19 Bielanski and Lalonde demonstrated that embryo cryopreservation may be regarded as a safety measure to decrease the potential risk or even mitigate disease transmission by embryo transfer.…”

Section: Methods For Virus Removal From Embryosmentioning

confidence: 98%

Bovine herpes virus-1: comparison of methods for removal of a commercially important pathogen from cattle sperm, oocytes and pre-implantation embryos

Robinson,

Sadeghzadeh

2019

JDVAR

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Bovine herpesvirus 1 (BoHV-1) is a pathogen of major veterinary importance, causing principally reproductive failure, genital and respiratory disease in cattle. Since embryo transfer is a rapidly growing commercial venture, BoHV-1 has a significant negative impact on cattle breeding by both natural and artificial service, and thereby on the global livestock industry. Clinical infection of the reproductive tract causes infertility, early embryonic death and abortion. BoHV-1 may infect an embryo by either of two means. The first is through entry of contaminated sperm into the oocyte at the point of fertilization, while the second is via contact with either contaminated follicular fluid, oviductal or uterine tissues. In addition, the virus may infect the recipient cow if an infected embryo is transferred by assisted reproduction technology. This article briefly examines the two principal methods that are routinely available to eliminate BoHV-1, performed in order to prevent infection of bovine embryos. Although each offers considerable benefits, it is also imperfect. Even after multiple trypsin washes BoHV-1 can adhere to the zona pellucida of oocytes and pre-implantation stage embryos; likewise, cryopreservation fails to eliminate all infectious virus particles. A more experimental technique, sperm processing, shows considerable promise but requires further validation as an effective way to remove BoHV-1 from bull semen before it can be recommended for industry-wide use.

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Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows

Cited by 2 publications

References 13 publications

Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1)

Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1)

Bovine herpes virus-1: comparison of methods for removal of a commercially important pathogen from cattle sperm, oocytes and pre-implantation embryos

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