Bovine IFNGR2, IL12RB1, IL12RB2, and IL23R polymorphisms and MAP infection status

Abstract

Mycobacterium avium ssp. paratuberculosis (MAP) infection causes a chronic granulomatous inflammatory condition of the bovine gut that is characterized by diarrhea, progressive weight loss, and emaciation, and ultimately leads to loss in productivity and profitability of dairy operations. The host cytokine machinery is known to play an important role in protecting against MAP infection. Therefore, the goal of the present study was to assess whether polymorphisms in candidate genes encoding important cytokines and cytokine receptors are associated with MAP infection status of dairy cattle. MAP infection status was evaluated based on serum and milk enzyme-linked immunosorbent assays (ELISAs) for MAP-specific antibodies. Twenty previously reported polymorphisms in genes encoding bovine interferon gamma (IFNG), IFNGR1, IFNGR2, IL22, IL22RA1, IL12RB1, IL12RB2, and IL23R were genotyped in a resource population of 446 dairy Holsteins with known MAP infection status, and logistic regression was used to assess the statistical association with a binomial MAP infection status phenotype. Four SNPs in IFNGR2, IL12RB1, IL12RB2, and IL23R were found to be associated with the MAP infection status of the resource population. These results underscore the importance of cytokines and their receptors in conferring protection against MAP infection and warrant further functional characterization of these associations.

Identification and genotyping of feline infectious peritonitis-associated single nucleotide polymorphisms in the feline interferon-γ gene

“…In recent decades, single nucleotide polymorphisms (SNP) in the IFN-γ gene (IFNG) have been found to be associated with various pathological conditions in humans [15] as well as in ruminants and fowl [16][17][18][19][20][21][22][23]. Nevertheless, this gene has not been investigated in cats.…”

Association of single nucleotide polymorphisms in IFNGR1 and IFNGR2 genes with bovine tuberculosis

“…At IFNGR1 SNP loci -56C/T loci, CC genotype was associated with TB in African populations (Cooke et al, 2006, Stein et al, 2007. However, non significant association of SNPs in IFNGR1 gene with brucellosis and MAP infection has been previously reported (Prakash et al, 2014, Pant et al, 2011. Hijjakata et al 2012found an association of one intronic region SNP i.e.…”

Association of genetic polymorphisms related to Johne’s disease with estimated breeding values of Holstein sires for milk ELISA test scores

“…Genome-wide association (GWAS) and candidate gene studies concerning JD have identified numerous single nucleotide polymorphisms (SNPs) across the bovine genome which are significantly associated with JD status in dairy cattle [9][10][11][12][13][14][15][16][17]. The identification of JD associated SNPs and the heritable nature of MAP infection reflect the genetic variation in susceptibility and resistance to JD.…”