Bovine kappa-casein gene polymorphism and its association with milk production traits

Rachagani, Satyanarayana; Gupta, I. D.

doi:10.1590/s1415-47572008005000001

Cited by 29 publications

(25 citation statements)

References 22 publications

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“…5, No. 2; lower compared to the reported figures of some other countries such as USA, Europe, Brazil and Japan which having a higher 'A' allele frequency at a range of 0.8-0.9 (Swaisgood, 1992;Rachagani & Gupta, 2008). On the other hand, Allmere et al (1998) and Oner and Elmaci (2006) reported the presence of 'A' and 'B' alleles for K-Ca, with highest frequency 'B' allele in Holstein breed.…”

Section: Resultsmentioning

confidence: 53%

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Gouda¹,

Galal²,

Abdel‐Aziz³

2013

JAS

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Kappa casein (K-Ca) genetic variations affected quality and composition of the milk. Several variants of Kappa casein (K-Ca) gene locus IV have been reported with special interest for the 'B' allele for its relation to the milk protein and fat yields. Genotyping and allelic frequencies of K-Ca among Native Egyptian breeds of cattle and buffalo were the aim of the present study. PCR amplification of DNA isolated from 300 blood samples collected from Holstein and Baladi cattle and buffalo were performed followed by restriction fragment length polymorphism using Hind-III restriction endonuclease (PCR-RFLP). Detection of 'AA' and 'AB' genotypes in cattle breeds, 'BB' and 'AB' in buffalo and two alleles 'A' and 'B' in the studied breeds. Molecular selection for animals carrying the 'B' allele could impact breeding programs for dairy production in native cattle and buffalo breeds in Egypt.

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Section: Resultsmentioning

confidence: 53%

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Gouda¹,

Galal²,

Abdel‐Aziz³

2013

JAS

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“…BB genotype showed a higher fat percentage (4.79±0.23) than AA and BB alleles which were 4.42±0.20% and 4.30±0.19%, respectively. In Sahiwal and Tharparker cattle, Rachagani et al (2008) found that BB genotypes of those cattle had higher Solid Non Fat (SNF) percentage and yield of monthly production than AA and AB genotypes. Another study of Deb et al (2014) reported that in Frieswal cattle, AB genotype had a significant higher total milk yield, peak yield and SNF for 300 days than AA genotypes.…”

Section: Resultsmentioning

confidence: 99%

“…The kappa casein molecule is a single chain polypeptide of 169 amino acids with a molecular weight of 19.2 kDa (Rachagani and Gupta, 2008). Kappa casein constitutes approximately 12% of total casein (Azevedo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority

Volkandari

Indriawati

Margawati

2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKKasein adalah protein susu yang terbagi dalam empat grup yaitu αS1, αS2, β-casein dan kappacasein (CSN3). Gen kappa-kasein berpengaruh terhadap pembentukan komponen dalam susu. Tujuan dari penelitian adalah mengidentifikasi polimorfisme gen kappa-kasein pada sapi perah Friesian Holstein (FH). Sebanyak 59 sampel sapi FH yaitu 32 asal Malang, 10 asal Sukahati Bogor dan 17 asal Pusat Penelitian Bioteknologi-Lembaga Ilmu Pengetahuan Indonesia (LIPI) (ternak koleksi) digunakan dalam penelitian. Sampel DNA diekstraksi dengan metode garam pekat dan dikuantifikasi menggunakan Spektrofotometer. Fragmen DNA spesifik gen kappa-kasein sepanjang 379 pb diamplifikasi menggunakan metode Polymerase Chain Reaction (PCR) dengan suhu annealing 56 o C dan 30 siklus. Teknik PCR-RFLP dengan enzim restriksi HindIII digunakan untuk analisis genotip. Hasil analisis menunjukkan bahwa terdapat tiga varian genotip yaitu AA, AB dan BB pada populasi Malang dan ternak koleksi Puslit Bioteknologi-LIPI sementara dua varian (AA dan BB) ditemukan pada populasi Sukahati. Rata-rata frekuensi genotip AA sebesar 65,28%, AB 31,72% dan BB 3,00% dengan rata-rata frekuensi alel A sebesar 0,81 dan B sebesar 0,19. Populasi sapi FH dalam keseimbangan genetik. Kesimpulan, polimorfisme gen kappa-kasein ditemukan pada ketiga populasi sapi FH dengan frekuensi alel A lebih tinggi dibanding alel B. Alel B diketahui berhubungan dengan produksi susu, komposisi susu dan cheese yield. Peningkatan alel B pada populasi FH berdampak terhadap performans produksi dan komponen susu. Eksplorasi (kuantitatif, kualitatif, dan molekuler) perlu dilakukan untuk aplikasi genetika molekuler pada seleksi sapi perah.Kata Kunci: gen kappa casein, polimorfisme, sapi perah, Friesian Holstein ABSTRACTCaseins are milk protein subdivided into four main groups which are αS1, αS2, β-casein and kappacasein (CSN3). Kappa-casein gene influences the manufacturing of milk properties. The aim of this study was to identify the kappa-casein gene polymorphism in Friesian Holstein (FH) cattle. Fifty nine (59) samples consisted of 32 (Malang), 10 (Sukahati Bogor) and 17 (Research Center for Biotechnology-Indonesian Institute of Sciences's collections) were applied in this study. DNA samples were extracted by high concentrated NaCl and quantified by spectrophotometer. The kappa-casein gene was amplified at 379 bp fragment by PCR method using a pair primer of kappa-casein at 56 o C annealing for 30 cycles. PCR-RFLP technique with HindIII was used for genotyping analysis. The result showed that there were three variants of genotypes (AA, AB and BB) in two populations from Malang and RC for Biotechnology-LIPI's collection while cattle from Sukahati had only AA and AB genotypes. The averages of genotype frequencies were 65.28%; 65.28%; and 3.00% for AA, AB and BB genotypes Polymorphism of Kappa-casein gene in FH cattle (S.D.Volkandari et al.) 213 respectively while frequencies of 0.81 and 0.19 were for A and B alleles, respectively. FH cattle populations were in equilibrium genetics. This finding...

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“…(Table 2) The association of HindIII-K-CN variants with milk related traits was confirmed in other studies carried out on several breeds (Denicourt et al, 1990). The allelic variants of the K-CN gene in Sahiwal and Tharparkar cattle breeds were analyzed (Rachagani and Gupta, 2008). The K-CN/BB genotype had more influence on the milk, fat, and protein yield in the Sahiwal cattle.…”

Section: K-cn Genementioning

confidence: 99%

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

Khaizaran¹,

Al-Razem²

2014

J. Cell Anim. Biol.

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Modern dairy cattle breeding strategies depend on linkage analysis and quantitative trait loci (QTL) of genes involved in milk yield and composition. This is because of their biological desired quantitative traits that play key roles in milk production. In this study, three genes directly related to milk production: prolactin (PRL), bovine kappa-casein (K-CN) and the pituitary-specific transcription factor (PIT-1) were analyzed in 144 cows. The aim of this study was to identify polymorphisms in the HolsteinFriesian cattle breed in Palestine in relation to the genetic markers and allelic variants of the three genes. Collection of samples depended on an experimental design that was completely randomized (CRD) and blood samples were collected from different cities across the West Bank, Palestine. The genotypes were determined through the polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) technique. The amplified fragments of PRL (294-bp), K-CN (530-bp) and PIT-1 (451-bp) were digested with RsaI, HindIII and HinfI, respectively. Statistical analysis found that the prolactin allelic substitution (AG, GG) played a role in milk production with a p-value of 0.00643 and α (0.001**), the AG allele of PRL being more favorable for milk production as compared to the GG allele. Genetic variants of the bovine K-CN gene played a role in milk production with a p-value of 0.04071 and α (0.01*), the AA allele possessing more positive effect than the BB and AB alleles. Similarly, the allelic substitution of the PIT-1 gene affected milk production with a p-value of 2.274e-05 and α (0***), the AA allele exercising a more positive effect followed by the AB and BB alleles, respectively. Among the three studied breeds (Friesian, hybrid and local), results show that the Friesian breed possesses higher overall milk production in Palestine as compared to the other two breeds.

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Bovine kappa-casein gene polymorphism and its association with milk production traits

Cited by 29 publications

References 22 publications

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

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