Bovine lactoferrin improves bone status of ovariectomized mice via immune function modulation

Malet, Arnaud; Bournaud, Elsa; Lan, Annaïg; Mikogami, Takashi; Tomé, Daniel; Blais, Anne

doi:10.1016/j.bone.2011.02.002

Cited by 36 publications

(28 citation statements)

References 65 publications

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“…It is well demonstrated that estrogen can promote bone growth. This function is also documented for lactoferrin (Blais et al, 2009;Malet et al, 2011). Because there are no significant differences in reproductive system among treatments in this study, therefore it seems likely that improvement in the bone status of bLF treatments is only related to in ovo injection of bLF that with a positive correlation is transferred to adult birds.…”

Section: Discussionsupporting

confidence: 60%

“…The role of this glycoprotein as a pleiotropic factor on cell growth and differentiation, embryonic development and potent antimicrobial and immuno-modulatory activities is well known, and other investigations have demonstrated that as a potent anabolic factor promotes bone growth (Brock, 2002;Malet et al, 2011). This glycoprotein stimulates osteoblast proliferation, reduces apoptosis of osteoblasts by 50% to 70%, and enhances thymidine incorporation into osteocytes.…”

Section: Introductionmentioning

confidence: 98%

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Effects of in ovo injection of bovine lactoferrin before incubation in layer breeder eggs on tibia measurements and performance of laying hens

Saki

Mahmoudi

2015

Animal

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There is increasing concern about welfare of laying hens in cages, and one aspect of this topic relates to bone fragility. Therefore, bone anabolic components such as bovine lactoferrin (bLF) may be an effective strategy to maintain the integrity and health of bones. A total of 1080 eggs were divided into four groups with three replicates, each comprising 270 eggs; (1) control group was injected with 100 μl of normal saline per egg; (2, 3 and 4) groups including 22.5 (low), 45 (medium) and 67.5 µg (high) of bLF in 100 µl of normal saline per egg. Eggs were incubated and after hatching, chicks were reared to 28 weeks of age. Tibia measurements were obtained at hatch and at 28 weeks of age. Tibia weight at hatch, was not influenced by in ovo injection of bLF in comparison with the control. Eggs injected with the high concentration of bLF (67.5 µg of bLF per egg) showed significant strengthening in laying-hen tibias at 28 weeks of age, as measured by ultimate force and bending stress, compared with the control. Egg weights from hens treated with this concentration of bLF were also significantly greater than the control. Our data suggest that tibia cortical thickness is a suitable variable for evaluating bone status reflecting bone integrity and strength. The present study also shows that bLF (67.5 µg of bLF per egg) injected into layer breeder eggs before incubation can be used to improve bone strength and egg weight of laying hens at 28 weeks of age, while having no detrimental effect on embryo hatchability.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 98%

Effects of in ovo injection of bovine lactoferrin before incubation in layer breeder eggs on tibia measurements and performance of laying hens

Saki

Mahmoudi

2015

Animal

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“…Few recent studies using ovariectomized (OVX) rodents as a model for post menopausal bone loss measured the effect of dietary supplementation on bone Guo et al, 2009;Malet et al, 2011). LF administered orally to OVX rats for three months protected them against the OVX-induced reduction of bone volume and BMD and increased the parameters of mechanical strength, increased bone formation and reduced bone resorption (Guo et al, 2009).…”

Section: Lactoferrinmentioning

confidence: 99%

“…These immune modulations were spatially and temporally correlated with reduced bone loss. We suggested that bLF modulates the inflammatory process via specific TNF inhibition in order to improve bone loss (Malet et al, 2011). Recently a clinical trial of 38 healthy postmenopausal women randomized into placebo or ribonuclease-enriched-LF (R-ELF) groups evaluated bone health status over a period of 12 months.…”

Section: Lactoferrinmentioning

confidence: 99%

Dietary Protein and Bone Health

Blais¹,

Rouy²,

Tomé³

2012

Dual Energy X-Ray Absorptiometry

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“…LF is not only present in milk and several other exocrine secretions, but it is also present in the secondary granules of neutrophils. Several biological functions have been described for LF, including iron homeostasis, immunomodulation, cellular growth and differentiation, antimicrobial, antioxidant, anti-inflammatory, antiviral and anticancer properties [5][6][7][8][9][10][11][12][13][14] . However, the mechanisms underlying these functions are not clear.…”

Section: Introductionmentioning

confidence: 99%

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Ge¹,

Liu²,

Chang³

et al. 2015

Front. Agr. Sci. Eng.

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Lactoferrin is a member of the transferrin family of multifunctional iron binding glycoproteins. While numerous physiological functions have been described for lactoferrin, the mechanisms underlying these functions are not clear. To further study the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of mice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression. Seven mice with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injecting the plasmid pX330, expressing a small guide RNA and human codonoptimized SpCas9, into fertilized eggs of mice. Plasmid integration and off-targeting of pX330 were not detected. These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method for targeted mutagenesis in mice.

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Bovine lactoferrin improves bone status of ovariectomized mice via immune function modulation

Cited by 36 publications

References 65 publications

Effects of in ovo injection of bovine lactoferrin before incubation in layer breeder eggs on tibia measurements and performance of laying hens

Effects of in ovo injection of bovine lactoferrin before incubation in layer breeder eggs on tibia measurements and performance of laying hens

Dietary Protein and Bone Health

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

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