Bovine Lyophilized Graft (Blg): Histological Analysis on Behavior in Humans After 49 Months

Abstract

Objective: To analyze the histological behavior of bovine lyophilized grafts (BLG) produced according to a protocol developed by the first author, in humans over a 49-month period by measuring the graft/bone neoformation ratio in relation to the total mineralized area. Methods: This was a case series involving 12 patients: eight females (66%) and four males (34%), totaling 13 biopsies. BLG was used, and surgical reintervention was subsequently required during the period 2000 to 2011. The slides produced were stained with hematoxylin-eosin (HE), were analyzed by a pathologist and were digitized for the proposed evaluation. Results: The mean age was 57 years and the mean follow-up was 49 months (range: 6-115). The average proportion of BLG was 42% (range: 13-85) and neoformed bone, 58% (range: 15-87) in relation to the total area mineralized. Conclusions: This study demonstrated that the BLG used presented osteoconductive characteristics and biocompatibility. BLG is a therapeutic option that can be used in orthopedic surgery in which bone defects need to be filled.

Reconstrução de região infraorbitária após ressecção de displasia fibrosa com enxerto ósseo bovino liofilizado

“…Não foram relatadas reações imunogênicas ou toxicidade (Trotta et al, 2014). O comportamento histológico do enxerto ósseo bovino liofilizado foi avaliado em humanos, em cirurgias ortopédicas mostrando excelente osteocondutividade biocompatibilidade (Diesel et al, 2017;Galia et al, 2012;Ribeiro et al, 2015,). No caso relatado, os controles tomográficos demonstraram a remodelação progressiva da área com a manutenção da arquitetura óssea e incorporação do enxerto ósseo, confirmados clinicamente pela ausência de rejeição/infecção.…”

“…O enxerto ósseo bovino liofilizado é biocompatível, de fácil obtenção, alta disponibilidade, e guarda grande semelhança ao osso humano. Permite modelagem da área a ser reconstruída, servindo como arcabouço osteocondutor (Galia et al, 2008;Galia et al, 2011;Galia et al, 2012;Ribeiro et al, 2015).…”

Bovine Grafting: An Effective Alternative after Curettage of Benign Bone Tumors

“…In addition, they are widely available in the market, have a long storage time, and are easy to handle in the operating theater. Some authors have expressed concern about antigenic potential and contamination by prions, but preparation and manufacture of this type of graft on an industrial scale have mitigated these risks [ 4 , 5 , 6 , 7 , 8 ].…”

“…Several studies in orthopedics and biomaterial science have described the biocompatibility, the histological characteristics of the newly formed bone after implantation, the compressive strength of bovine bone grafts, and their successful use in hip arthroplasties, knee osteotomies, and foot arthrodesis [ 4 , 6 , 7 , 10 , 11 , 12 , 13 , 14 , 15 ]. We, therefore, reviewed a series of patients with benign bone tumors or pseudotumors treated with curettage, a local adjuvant (when necessary), and filling with freeze-dried bovine bone graft.…”