Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Fenyves, A; Saxer, Markus; Spanel‐Borowski, Katharina

doi:10.1007/bf01984942

Cited by 17 publications

(17 citation statements)

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“…Of note is the fact that this morphological change also occurred with IFNG treatment alone; but similar to CLENDOs of pregnancy [29], did not induce cell death. IFNG-induced phenotypic changes of CLENDOs have also been reported by others [41,42]. We suggest IFNG negatively influences CLENDOs of the estrous cycle (triggers cell senescence?)…”

Section: Endothelial Cells Of the Bovine Corpus Luteumsupporting

confidence: 85%

Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Cherry

Hou

Rueda

et al. 2008

Journal of Reproduction and Development

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Abstract. Endothelial cells derived from the corpus luteum (CLENDOs) exhibit diverse characteristics presumably serving their wide-ranging roles in luteal function and fate. Here, several attributes of CLENDOs derived from cows at midcycle (days 9-12 of the estrous cycle) were compared with CLENDOs from early pregnancy (day 60 of pregnancy). Flow cytometric analysis of cells fluorescently-tagged with the lectins Bandeiraea simplicifolia (BS-1) and Concanavalin A (ConA) indicated that CLENDOs of midcycle CL do not differ from those of pregnancy. Mean fluorescence intensity for BS-1 was 15 ± 1 and 23 ± 7 fluorescent units for midcycle CLENDOs and CLENDOs of pregnancy, respectively (P>0.05). For ConA, mean fluorescence was 25 ± 2 and 26 ± 1 fluorescent units, respectively (P>0.05). The CLENDOs were also exposed to cytokines to assess differences in activation of nuclear factor kappa B signaling (NF-κB), induction of the transcription factor interferon regulatory factor 1 (IRF1), cytokine production, and cytokine-induced cell death. In response to TNF, for instance, both types of CLENDOs exhibited a rapid, 5-fold decrease in NF-κB inhibitor alpha (NFKBIA) protein expression (P<0.05), and a 4-fold increase in IRF1 expression (P<0.05), that did not differ with phenotype (P>0.05). Similarly, both types of CLENDOs produced tumor necrosis factor alpha and chemokine ligand 2 in response to IFNG stimulation (P<0.05) that did not differ with phenotype (P>0.05). Lastly, extended exposure of CLENDOs of midcycle CL to cytokines induced cell death (~50% cell death vs. control) similar to the incidence of cell death seen previously in CLENDOs of early pregnancy. The results indicate that several physical and functional characteristics of CLENDOs of midcycle CL are retained through early pregnancy, including lectin-binding properties, sensitivity to cytokines, and the activation of cytokine-initiated intracellular signals. Key words: Bovine, Corpus luteum, Cytokine, Endothelial cell, Lectin (J. Reprod. Dev. 54: [183][184][185][186][187][188][189][190][191] 2008) he corpus luteum (CL) is a temporary endocrine gland that forms within the ovary following ovulation and contributes to estrous/menstrual cycle regularity and the establishment of early pregnancy [1]. Aside from its transient nature, an intriguing aspect of the CL is its adaptable lifespan. In domestic livestock, the CL remains functional for as few as 15-18 days to as many as 200-300 days depending upon species and reproductive status of the animal [2]. In the cow, the CL develops, functions, and begins to regress within 17-18 days of ovulation during the estrous cycle, but retains a functional lifespan of more than 200 days during pregnancy [3] . Similarly, luteal lifespan in the ewe ranges from 15-16 days during the estrous cycle to more than 50 days during pregnancy [4]. In fact in most placental mammals the continuation of luteal function associated with pregnancy extends well beyond the period of a single estrous or menstrual cycle and is required for the establi...

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Section: Endothelial Cells Of the Bovine Corpus Luteumsupporting

confidence: 85%

Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Cherry

Hou

Rueda

et al. 2008

Journal of Reproduction and Development

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“…The five different endothelial cell types derived from bovine corpus luteum are shown by phase contrast microscopy as described by Spanel-Borowski and van der Bosch [41] and Fenyves et al ., [44]. Panels a–f: In cytokeratin-positive cell types 1 and 2, the cobble-stone like pattern is distinct (panels a and b); in cytokeratin-negative cells, the monlayer consists of spindle-like cells with prominent "vacuoles" in cell type 3 (panel c); the monlayer of polygonal opaque cells appear in cell type 4 (panel d).…”

Section: Endothelial Cells Of the Corpus Luteummentioning

confidence: 99%

“…Panels c-d: Immunofluorescence for actin filaments using phalloidin-FITC. The cytokeratin-positive (CK-positive) type 1 cells demonstrate a peripheral band of microfilaments (panel c); the cytokeratin-negative (CK-negative) cells of type 3 show a starburst-like pattern (panel d) [44]. …”

Section: Endothelial Cells Of the Corpus Luteummentioning

confidence: 99%

Microvascular endothelial cells of the corpus luteum

Davis

Rueda

Spanel-Borowski

2003

Reprod Biol Endocrinol

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The cyclic nature of the capillary bed in the corpus luteum offers a unique experimental model to examine the life cycle of endothelial cells, involving discrete physiologically regulated steps of angiogenesis, blood vessel maturation and blood vessel regression. The granulosa cells and theca cells of the developing antral follicle and the steroidogenic cells of the corpus luteum produce and respond to angiogenic factors and vasoactive peptides. Following ovulation the neovascularization during the early stages of corpus luteum development has been compared to the rapid angiogenesis observed during tumor formation. On the other end of the spectrum, the microvascular endothelial cells are the first cells to undergo apoptosis at the onset of corpus luteum regression. Important insights on the morphology and function of luteal endothelial cells have been gained from a combination of in vitro and in vivo studies on endothelial cells. Endothelial cells communicate with cells comprising the functional unit of the corpus luteum, i.e., other vascular cells, steroidogenic cells, and immune cells. This review is designed to provide an overview of the types of endothelial cells present in the corpus luteum and their involvement in corpus luteum development and regression. Available evidence indicates that microvascular endothelial cells of the corpus luteum are not alike, and may differ during the process of angiogenesis and angioregression. The contributions of vasoactive peptides generated by the luteal endothelin-1 and the renin-angiotensin systems are discussed in context with the function of endothelial cells during corpus luteum formation and regression. The ability of two cytokines, tumor necrosis factor alpha and interferon gamma, are evaluated as paracrine mediators of endothelial cell function during angioregression. Finally, chemokines are discussed as a vital endothelial cell secretory products that contribute to the recruitment of eosinophils and macrophages. The review highlights areas for future investigation of ovarian microvascular endothelial cells. The potential clinical applications of research directed on corpus luteum endothelial cells are intriguing considering reproductive processes in which vascular dysfunctions may play a role such as ovarian failure, polycystic ovary syndrome (PCOS), and ovarian hyperstimulation syndrome (OHSS).

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“…Moreover, the microvascular endothelial cells within a tissue can exhibit strikingly different phenotypic and functional characteristics [13]. Evidence for heterogeneity of microvascular endothelial cells was previously observed in the rat and bovine ovaries [7,18,50]. Capillary endothelial cells in the rat ovary display an enhanced ability to bind hepatic lipase [7].…”

mentioning

confidence: 96%

Isolation and Culture of Microvascular Endothelial Cells from the Primate Corpus Luteum1

Christenson

Stouffer

1996

Biology of Reproduction

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Endothelial cells have common as well as specialized roles in different tissues and organs; as such, the abundant endothelial cells in the corpus luteum (> 50% of total cell population) could have unique activities necessary for luteal function. Our objective was to establish a method for isolating a pure population of endothelial cells from the primate corpus luteum and to determine the basic conditions for in vitro culture. Corpora lutea collected from rhesus monkeys throughout the luteal phase of the menstrual cycle were minced and enzymatically dispersed into single cell suspensions. Endothelial cells were isolated from the remaining cells (i.e., steroidogenic, fibroblastic, etc.) utilizing magnetic beads labeled with a lectin, Ulex europaeus agglutinin-1 (UEA-1), which binds a sugar found only on primate endothelial cells. After exposure to a magnetic field, UEA-1-negative (-) cells were decanted from the pelleted UEA-1-positive (+) cells; to remove beads from the UEA-1 (+) cells, excess sugar was applied. After optimization of the bead-to-cell ratio, the UEA-1 (+) group contained a population of cells 8-12 microns in diameter (typical endothelial cell size) and < 1% steroidogenic cells. UEA-1 (-) cells were larger (15-35 microns) and stained histochemically for the steroidogenic marker, 3 beta-hydroxysteroid dehydrogenase. Immunocytochemical analysis demonstrated that > 93% of all cells in the UEA-1 (+) group stained positive for the specific endothelial cell marker, platelet/endothelial cell adhesion molecule-1. Cultured UEA-1 (+) cells produced low levels of progesterone and were unresponsive to hCG (100 ng/ml). In contrast, cultured UEA-1 (-) and mixed (unsorted) cells produced high basal levels of progesterone and exhibited a > 3-fold increase in response to hCG treatment. Preliminary experiments comparing different culture media and matrices demonstrated that 1) cell proliferation was unaffected by type of medium (i.e., Dulbecco's Modified Eagle Medium [DMEM]/ F12 or McCoy's 5A); 2) the presence of serum was essential in the absence of added growth factors, and 3) extracellular matrix had a profound effect on proliferation. UEA-1 (+) cells exhibited a dose-dependent increase in cell proliferation in response to vascular endothelial growth factor (VEGF) in the presence and absence of fetal calf serum. In the absence of serum, VEGF stimulated proliferation of UEA-1 (+) cells plated on fibronectin but not collagen I, whereas in the presence of 10% fetal calf serum, both matrices supported VEGF-induced mitogenesis. These studies provide for the first time an efficient, reliable method for isolating primate luteal endothelial cells and describe in vitro culture conditions for subsequent studies examining luteal endothelial cell function and regulation.

show abstract

Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Cited by 17 publications

References 27 publications

Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Microvascular endothelial cells of the corpus luteum

Isolation and Culture of Microvascular Endothelial Cells from the Primate Corpus Luteum1

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