Bovine oocytes from early antral follicles grow to meiotic competence in vitro: Effect of FSH and hypoxanthine

Harada, Mayuko; Miyano, Takashi; Matsumura, K.; Osaki, Susumu; Miyake, Masakazu; Kato, Seishiro

doi:10.1016/s0093-691x(97)00298-7

Cited by 65 publications

(76 citation statements)

References 22 publications

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“…All queens weighed more than 2.2 kg. Ovaries were kept in normal saline at room temperature, delivered to the laboratory within 4 h after collection, and subsequently transferred to TCM-199 (pH 7.4; Gibco Laboratories, Grand Island, NY, USA) containing 0.1% polyvinylalcohol, 25 mM HEPES, 0.85 mg/mL of NaHCO3, and 50 μg/mL of gentamicin sulfate (isolation medium) [18]. Ovaries were cut (with scissors) along their long axis.…”

Section: Oocyte Recoverymentioning

confidence: 99%

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

et al. 2008

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This study was conducted to improve in vitro production of embryos from domestic cats using TCM-199 as an IVM medium. The time sequence of nuclear maturation and the optimal timing of in vitro insemination were examined. Most oocytes were at the germinal vesicle stage immediately after collection; however, 8.3% had already resumed meiosis before IVM culture. After 30 h of IVM culture, the percentage of oocytes at metaphase II (MII) reached a peak (75.5%) and did not change (P>0.05) from 30 to 48 h after IVM culture. The percentage of oocytes with two pronuclei was higher (P<0.05) for oocytes matured for 30 and 36 h (38.2 and 33.0%, respectively) than for those after IVM culture for only 24 h (18.5%).Total sperm penetration rate was highest (P<0.05) for oocytes that had been matured for 30 h (46.1%). After 30 h of IVM and 18 h of IVF culture, 66.3 and 24.8% of inseminated oocytes had cleaved and developed to the blastocyst stage, respectively. We concluded that IVM of feline oocytes for 30 h in TCM-199 resulted in optimal nuclear maturation and sperm penetration.

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Section: Oocyte Recoverymentioning

confidence: 99%

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

et al. 2008

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“…FSH has been reported to be crucial in follicle survival and granulosa cell proliferation in a culture of mouse preantral follicles (Spears et al, 1998). FSH supplementation in a 3-D culture system of bovine oocytes stimulates the proliferation of granulosa cells that maintain their morphological qualities, consequently allowing AFLS formation and the maintenance of gap junctional communication between oocyte and cumulus cells (Harada et al, 1997). The addition of a low concentration of LH during in vitro follicular culture enhances antral cavity formation and improves the meiotic competence of oocytes (Cortvrindt et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The AFLS formation relates to the proliferation of cumulus/granulosa cells surrounding oocytes and the viability of oocytes (Harada et al, 1997). Therefore, only oocytes were recovered from the collagen gels with AFLS formation at the end of culture for 8 days.…”

Section: Measurement Of Diameter Of Afls and Meiotic Status Of Oocytesmentioning

confidence: 99%

“…When the 3-D organisation of cumulus granulosa cells around the oocyte was maintained during oocyte culture, COCs formed an antral follicle-like structure (AFLS) and grew in vitro (Harada et al, 1997). The formation of an AFLS is dependent on the proliferation and differentiation of cumulus granulosa…”

mentioning

confidence: 99%

“…LH promotes the production of androgens, which are aromatised in the granulosa layer under the influence of FSH (Evans et al, 1981). Moreover, FSH stimulates follicle survival and granulosa cell proliferation in culture of pre-antral follicles (Cortvrindt et al, 1998;Spears et al, 1998) and small oocytes from early antral follicles (Harada et al, 1997). FSH is thought to affect granulosa cells by activating adenylyl cyclase activity and promoting cAMP accumulation (Kolena and Channing, 1972).…”

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Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Ono

Takaoka

Morita

et al. 2015

Acta Veterinaria Hungarica

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This study evaluated the effect of dibutyryl cyclic adenosine monophosphate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus-oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4-8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5-2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1-100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Müllerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.Key words: Antrum-like structures, cAMP, cumulus cells, gap junction, follicular formation Three-dimensional (3-D) culture of cumulus-oocyte complexes (COCs) may provide a valuable approach for studying the regulation of follicular development. When the 3-D organisation of cumulus granulosa cells around the oocyte was maintained during oocyte culture, COCs formed an antral follicle-like structure (AFLS) and grew in vitro (Harada et al., 1997). The formation of an AFLS is dependent on the proliferation and differentiation of cumulus granulosa

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Interaction between growing oocytes and granulosa cells in vitro

Alam

Miyano

2019

Reprod Medicine & Biology

127

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BackgroundOocyte growth is accompanied by follicular development in mammalian ovaries. Since the discovery of two oocyte‐derived factors, growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15), knowledge of the bidirectional communication between oocytes and granulosa cells for ovarian function and fertility has been accumulated. In addition, the growth culture system of oocytes has been improved, further promoting the studies on the communication between oocytes and granulosa cells in vitro.MethodsWe provide an overview of the role of granulosa cells in oocyte growth and the role of oocytes in follicular development along with our recent findings in culture experiments of bovine growing oocytes.Main findingsGranulosa cells supply nutrients and metabolites through gap junctions to oocytes and secrete paracrine signals to regulate oocytes. Oocytes regulate granulosa cell proliferation and differentiation and induce antrum formation via GDF9 and BMP15.ConclusionOocytes actively participate in various aspects of follicular development, including antrum formation via the oocyte‐derived factors GDF9 and BMP15, whose synthesis is probably regulated by granulosa cells. In vitro studies will reveal the precise communication loop between oocytes and granulosa cells that facilitates the coordinated development of oocytes and granulosa cells in the follicles.

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Bovine oocytes from early antral follicles grow to meiotic competence in vitro: Effect of FSH and hypoxanthine

Cited by 65 publications

References 22 publications

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Interaction between growing oocytes and granulosa cells in vitro

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