Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector.

Sarver, Nava; Gruß, Peter; Law, Ming-Fan; Khoury, George; Howley, Peter M.

doi:10.1128/mcb.1.6.486

Cited by 173 publications

(93 citation statements)

References 35 publications

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“…With regard to copy number (20-200 copies) and the absence of integrated sequences, our results are in agreement with those reported for the BPV vector system by other investigators (Sarver et al, 1981 has also been observed previously and is thought to be caused by inhibitory sequences present in the bacterial vector (Binetruy et al, 1982). Therefore, we removed most of the bacterial sequences from our recombinants by restriction enzyme cleavage prior to transfection.…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Secretion of the hepatitis B virus surface antigen from mouse cells using an extra-chromosomal eucaryotic vector.

Stenlund¹,

Lamy²,

Moreno‐López³

et al. 1983

The EMBO Journal

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Recombinant DNA molecules which contained a subgenomic fragment of the hepatitis B virus (HBV) genome, the pML2 vector and the bovine papillomavirus type 1 (BPV) genome were constructed. The HBV fragment includes the entire transcription unit for the hepatitis B surface antigen (HBsAg). After propagation in Escherichia coli, the recombinant plasmids were cleaved with endonucleases SalI and PvuI to eliminate most of the bacterial sequences before transfection of mouse C127 cells. Foci were observed 10–14 days after transfection. Cells from selected foci were cloned and the supernatants were assayed for the presence of HBsAg. Most of the clones tested were found to secrete HBsAg particles into the growth medium. These particles appear to be similar to the 22 nm particles present in the serum of HBV chronic carriers. SDS‐polyacrylamide gel electrophoresis revealed that the particles contain two polypeptides, probably representing the glycosylated and unglycosylated forms of the HBsAg major polypeptide. An analysis of DNA from the transformed clones revealed that they contain multiple extra‐chromosomal copies of the recombinant, which, however, had suffered rearrangement.

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Section: Discussionsupporting

confidence: 83%

“…It is thus difficult to establish cell lines for continuous synthesis of polypeptides using such vectors. Sarver et al (1981) have recently reported that the bovine papilloma virus (BPV) genome can be used as 40n leave of absence from: Institut Merieux, Marcy 1'etoile, 69260 Lyons, France. *To whom reprint requests should be sent.…”

Section: Introductionmentioning

confidence: 99%

Secretion of the hepatitis B virus surface antigen from mouse cells using an extra-chromosomal eucaryotic vector.

Stenlund¹,

Lamy²,

Moreno‐López³

et al. 1983

The EMBO Journal

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show abstract

“…The virus or its molecularly cloned DNA can transform certain mouse cells in vitro, and the DNA persists as an extrachromosomal multicopy plasmid in the transformed cells (1). A 69% subgenomic fragment (BPV69T) bounded by the unique HindIII and BamHI sites is sufficient to induce transformation in vitro (2), and recombinants containing this DNA segment can be maintained as free plasmids in transformed cells (3). Cell lines established from transformed foci have a fully transformed phenotype in that they are anchorage independent and are tumorigenic in nude mice (4).…”

mentioning

confidence: 99%

Bovine papillomavirus contains multiple transforming genes.

Yi¹,

Okayama²,

Howley³

1985

Proc. Natl. Acad. Sci. U.S.A.

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232

202

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Bovine papillomavirus type 1 (BPV-1) and its cloned full-length DNA can transform rodent cells in vitro, and the viral DNA persists as an extrachromosomal multicopy plasmid in these transformed cells. Previous studies have identified at least five discrete viral RNAs that are expressed in BPV-1 transformed cells and have shown that these transcripts share a 3' coterminus. To further define the structure of these RNAs and to characterize the functions of individual viral transcripts, we constructed a cDNA library with mRNA from BPV-1-transformed mouse C127 cells using an Okayama and Berg plasmid. From a library of 105 independent clones, 200 BPV-1 specific clones were isolated and characterized. Sequence analysis has revealed differential splicing patterns for the mRNA species in BPV-1 transformed cells. In conjunction with the open reading frames (ORFs) deduced from the BPV-1 DNA sequence, it is possible to predict the structure of the potential encoded proteins. The vector used to generate these cDNA clones contains mammalian cell transcriptional regulatory elements, facilitating their functional characterization. We have identified two distinct classes of cDNA clones that can each independently transform mouse C127 cells. One class of cDNA clones contains the-E2 ORF intact and the second contains the E6 ORF intact. These two putative viral functions appear to act synergistically in transforming mouse C127 cells in vitro.fied several regions of BPV-1 genome that influence the expression of the viral transforming functions (9-12). Deletion mutants affecting the E2 ORF are significantly impaired in their ability to transform mouse C127 cells, suggesting that the putative E2 protein is an important transforming protein (9). The expression of E2 alone, however, is not sufficient for the fully transformed phenotype. Deletion mutagenesis studies map an additional function that is critical for the fully transformed phenotype to the region between the Hpa I site (base 1) and the Sma I site (base 945) (9). These previous studies did not distinguish whether these two distinct regions of the viral genome encode separate proteins or whether they contain exons that are spliced together at the level of mRNA to generate a single transforming protein.To further define the structure of the mRNAs in transformed cells and to characterize the functions encoded by individual viral transcripts, we constructed a cDNA library using mRNA from BPV-1-transformed mouse cells. The vector used for cDNA cloning contains the simian virus 40 (SV40) early promoter, the SV40 late region introns, and the SV40 late region polyadenylylation site, allowing expression of cDNA clones in mouse cells for functional analysis (17). In this study, expression vectors that carried individual cDNA inserts were tested for their ability to transform mouse C127 cells.

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“…Advantages of the BPV vector system are that the transformed cell lines are stable, the cells contain multiple copies of the BPV hybrid DNA, and these inserted gene segments are maintained in a defined and homogeneous sequence environment. The BPV vector system has been successfully used to express the rat preproinsulin gene rIh (22), the human P-globin gene (4), and the Escherichia coli xanthine guanine phosphoribosyltransferase gene (11) in mouse cells. In each of these systems, a constitutive production of RNA has been demonstrated from the inserted gene, but to date the regulated expression of a gene inserted in a BPV vector and maintained extrachromosomally has not been reported.…”

mentioning

confidence: 99%

Inducible expression of the human interferon beta 1 gene linked to a bovine papilloma virus DNA vector and maintained extrachromosomally in mouse cells.

Mitrani-Rosenbaum¹,

Maroteaux²,

Mory³

et al. 1983

Mol. Cell. Biol.

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Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector.

Cited by 173 publications

References 35 publications

Secretion of the hepatitis B virus surface antigen from mouse cells using an extra-chromosomal eucaryotic vector.

Secretion of the hepatitis B virus surface antigen from mouse cells using an extra-chromosomal eucaryotic vector.

Bovine papillomavirus contains multiple transforming genes.

Inducible expression of the human interferon beta 1 gene linked to a bovine papilloma virus DNA vector and maintained extrachromosomally in mouse cells.

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