Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

Shklover, Jeny; McMasters, James; Alfonso-García, Alba; Higuita, Manuela Lopera; Panitch, Alyssa; Marcu, Laura; Griffiths, Leigh G.

doi:10.1038/s41598-019-53230-1

Cited by 11 publications

(8 citation statements)

References 47 publications

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“…To answer this question, endothelial cells were seeded on the fibrous layer of the GA-treated pericardium, which in the native valve is the most proximal layer contacting blood [29]. Then, the number of cells was compared to that of HUVEC adhering on the BP control membranes after 4 days of culture, as performed by others on bovine pericardium substrates [30,31]. Endothelial cells have been chosen because, among the different cell populations, they better mimic a physiological environment in cardiovascular applications [19].…”

Section: Discussionmentioning

confidence: 99%

Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction

Gardin

Morciano

Ferroni

et al. 2021

JCM

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Background: The Ozaki procedure is an innovative surgical technique aiming at reconstructing aortic valves with human autologous pericardium. Even if this procedure is widely used, a comprehensive biological characterization of the glutaraldehyde (GA)-fixed pericardial tissue is still missing. Methods: Morphological analysis was performed to assess the general organization of pericardium subjected to the Ozaki procedure (post-Ozaki) in comparison to native tissue (pre-Ozaki). The effect of GA treatment on cell viability and nuclear morphology was then investigated in whole biopsies and a cytotoxicity assay was executed to assess the biocompatibility of pericardium. Finally, human umbilical vein endothelial cells were seeded on post-Ozaki samples to evaluate the influence of GA in modulating the endothelialization ability in vitro and the production of pro-inflammatory mediators. Results: The Ozaki procedure alters the arrangement of collagen and elastic fibers in the extracellular matrix and results in a significant reduction in cell viability compared to native tissue. GA treatment, however, is not cytotoxic to murine fibroblasts as compared to a commercially available bovine pericardium membrane. In addition, in in vitro experiments of endothelial cell adhesion, no difference in the inflammatory mediators with respect to the commercial patch was found. Conclusions: The Ozaki procedure, despite alteration of ECM organization and cell devitalization, allows for the establishment of a noncytotoxic environment in which endothelial cell repopulation occurs.

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Section: Discussionmentioning

confidence: 99%

Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction

Gardin

Morciano

Ferroni

et al. 2021

JCM

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“…Formular for RGR of test sample (%) was as (OD test /OD blank ) × 100%. The liquid extract is confirmed as no-toxic to the cells if the RGR value is higher than 70 %, according to ISO 10993-5 guidance [ 2 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bovine pericardium, first utilized for bioprosthetic heart valve fabrication, has been widely used in clinical practice [ 1 , 2 ]. Decellularization is considered the critical factor that significantly promotes bovine pericardium and other tissue-derived extracellular matrix (ECM) for biomedical research and application.…”

Section: Introductionmentioning

confidence: 99%

In-Vitro Endothelialization Assessment of Heparinized Bovine Pericardial Scaffold for Cardiovascular Application

Nguyen

Tran

2022

Polymers

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(1) Background: Hemocompatibility is a critical challenge for tissue-derived biomaterial when directly contacting the bloodstream. In addition to surface modification with heparin, endothelialization of the grafted material is suggested to improve long-term clinical efficacy. This study aimed to evaluate the ability to endothelialize in vitro of heparinized bovine pericardial scaffolds. (2) Methods: bovine pericardial scaffolds were fabricated and heparinized using a layer-by-layer assembly technique. The heparinized scaffolds were characterized for heparin content, surface morphology, and blood compatibility. Liquid extraction of the samples was prepared for cytotoxicity testing on human endothelial cells. The in-vitro endothelialization was determined via human endothelial cell attachment and proliferation on the scaffold. (3) Results: The heparinized bovine pericardial scaffold exhibited a heparin coating within its microfiber network. The scaffold surface immobilized with heparin performed good anti-thrombosis and prevented platelet adherence. The proper cytotoxicity impact was observed for a freshly used heparinized sample. After 24 h washing in PBS 1X, the cell compatibility of the heparinized scaffolds was improved. In-vitro examination results exhibited human endothelial cell attachment and proliferation for 7 days of culture. (4) Conclusions: Our in-vitro analysis provided evidence for the scaffold’s ability to support endothelialization, which benefits long-term thromboresistance.

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“…The predominant cell is the pericardial fibroblast. The serous pericardium is composed of mesothelium (epithelial-like cells) with its basal lamina overlying a thin layer of loose connective tissue ( 25 ). The mesothelial cells form a monolayer lining in the visceral pericardium ( 26 ), which plays an important role in inflammation and tissue repair ( 27 ).…”

Section: The Structure and Properties Of Natural Pericardiummentioning

confidence: 99%

Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves

Williams

Bezuidenhout

Villiers

et al. 2021

Front. Cardiovasc. Med.

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The use of bioprostheses for heart valve therapy has gradually evolved over several decades and both surgical and transcatheter devices are now highly successful. The rapid expansion of the transcatheter concept has clearly placed a significant onus on the need for improved production methods, particularly the pre-treatment of bovine pericardium. Two of the difficulties associated with the biocompatibility of bioprosthetic valves are the possibilities of immune responses and calcification, which have led to either catastrophic failure or slow dystrophic changes. These have been addressed by evolutionary trends in cross-linking and decellularization techniques and, over the last two decades, the improvements have resulted in somewhat greater durability. However, as the need to consider the use of bioprosthetic valves in younger patients has become an important clinical and sociological issue, the requirement for even greater longevity and safety is now paramount. This is especially true with respect to potential therapies for young people who are afflicted by rheumatic heart disease, mostly in low- to middle-income countries, for whom no clinically acceptable and cost-effective treatments currently exist. To extend longevity to this new level, it has been necessary to evaluate the mechanisms of pericardium biocompatibility, with special emphasis on the interplay between cross-linking, decellularization and anti-immunogenicity processes. These mechanisms are reviewed in this paper. On the basis of a better understanding of these mechanisms, a few alternative treatment protocols have been developed in the last few years. The most promising protocol here is based on a carefully designed combination of phases of tissue-protective decellularization with a finely-titrated cross-linking sequence. Such refined protocols offer considerable potential in the progress toward superior longevity of pericardial heart valves and introduce a scientific dimension beyond the largely disappointing ‘anti-calcification’ treatments of past decades.

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Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

Cited by 11 publications

References 47 publications

Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction

Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction

In-Vitro Endothelialization Assessment of Heparinized Bovine Pericardial Scaffold for Cardiovascular Application

Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves

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