Bovine Platelet Lysate as an Alternative Growth Supplement for In Vitro Culture of Triple-Negative Breast Cancer Cells

Abstract

Cell culture media and associated supplements are vital for the proper growth and development of cells for in vitro cell culture. Fetal bovine serum and human platelet lysate are common additives to cell culture media to promote proliferation. Bovine platelet lysate is a novel supplement obtained from bovine plasma that has the potential to become a cheaper, more ethical alternative to fetal bovine serum and human platelet lysate. In this study, we investigated the proliferative effects of DMEM cell culture media supplemented with either fetal bovine serum, human platelet lysate or bovine platelet lysate for triple-negative breast cancer (MDA-MB-231) cells. For each differentially supplemented media, proliferation, cell diameter, and wound healing capabilities were assessed. We show that cells grown in bovine platelet lysate had a lower proliferation rate than cells grown in either fetal bovine serum or human platelet lysate but had larger cell diameters and comparable wound healing rates.