Bovine Polledness – An Autosomal Dominant Trait with Allelic Heterogeneity

Međugorac, Ivica; Seichter, Doris; Graf, Alexander; Ruß, Ingolf; Blum, Helmut; Göpel, Karl Heinrich; Rothammer, Sophie; Förster, M.; Krebs, Stefan

doi:10.1371/journal.pone.0039477

Cited by 126 publications

(170 citation statements)

References 38 publications

(69 reference statements)

Supporting

Mentioning

165

Contrasting

Unclassified

Order By: Relevance

“…P 5ID and P 80kbID (Figure 1A) using previously described methods [4]. A small proportion of these animals was also genotyped for the three other mutations in the Friesian haplotype i.e.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin

et al. 2014

Self Cite

View full text Add to dashboard Cite

BackgroundThe absence of horns, called polled phenotype, is the favored trait in modern cattle husbandry. To date, polled cattle are obtained primarily by dehorning calves. Dehorning is a practice that raises animal welfare issues, which can be addressed by selecting for genetically hornless cattle. In the past 20 years, there have been many studies worldwide to identify unique genetic markers in complete association with the polled trait in cattle and recently, two different alleles at the POLLED locus, both resulting in the absence of horns, were reported: (1) the Celtic allele, which is responsible for the polled phenotype in most breeds and for which a single candidate mutation was detected and (2) the Friesian allele, which is responsible for the polled phenotype predominantly in the Holstein-Friesian breed and in a few other breeds, but for which five candidate mutations were identified in a 260-kb haplotype. Further studies based on genome-wide sequencing and high-density SNP (single nucleotide polymorphism) genotyping confirmed the existence of the Celtic and Friesian variants and narrowed down the causal Friesian haplotype to an interval of 145 kb.ResultsAlmost 6000 animals were genetically tested for the polled trait and we detected a recombinant animal which enabled us to reduce the Friesian POLLED haplotype to a single causal mutation, namely a 80-kb duplication. Moreover, our results clearly disagree with the recently reported perfect co-segregation of the POLLED mutation and a SNP at position 1 390 292 bp on bovine chromosome 1 in the Holstein-Friesian population.ConclusionWe conclude that the 80-kb duplication, as the only remaining variant within the shortened Friesian haplotype, represents the most likely causal mutation for the polled phenotype of Friesian origin.

show abstract

“…P 5ID and P 80kbID (Figure 1A) using previously described methods [4]. A small proportion of these animals was also genotyped for the three other mutations in the Friesian haplotype i.e.…”

Section: Methodsmentioning

confidence: 99%

“…In the past years, the position of the POLLED locus on BTA1 was refined [11,12] and candidate causal mutations were identified [2,4,5]. Recently, Medugorac et al [4] demonstrated the existence of at least two different alleles at the POLLED locus in cattle.…”

Section: Introductionmentioning

confidence: 99%

The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Especially taurine cattle adapted to a wide range of climates, even including Siberian conditions. The acquisition of adaptive traits can now be investigated by studying the breed distribution of causative mutations [227][228][229]. A survey of the events preceding the development of specialized breeds:…”

Section: On the History And Future Of Diversitymentioning

confidence: 99%

On the History of Cattle Genetic Resources

Felius¹,

Beerling²,

et al. 2014

View full text Add to dashboard Cite

Cattle are our most important livestock species because of their production and role in human culture. Many breeds that differ in appearance, performance and environmental adaptation are kept on all inhabited continents, but the historic origin of the diverse phenotypes is not always clear. We give an account of the history of cattle by integrating archaeological record and pictorial or written sources, scarce until 300 years ago, with the recent contributions of DNA analysis. We describe the domestication of their wild ancestor, migrations to eventually all inhabited continents, the developments during prehistory, the antiquity and the Middle Ages, the relatively recent breed formation, the industrial cattle husbandry in the Old and New World and the current efforts to preserve the cattle genetic resources. Surveying the available information, we propose three main and overlapping phases during the development of the present genetic diversity: (i) domestication and subsequent wild introgression; (ii) natural adaptation to a diverse agricultural habitat; and (iii) breed development.

show abstract

“…Two allelic variants conferring polledness have been identified on chromosome 1 (28). Meiotic introgression of the POLLED trait into horned dairy breeds can be accomplished by traditional crossbreeding, but the genetic merit of the resulting animals would rank lower owing to the admixture of unselected (inferior) alleles for net merit (i.e., milk production) into the population.…”

Section: Significancementioning

confidence: 99%

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

Tan

Carlson

Lancto

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

259

196

View full text Add to dashboard Cite

We have expanded the livestock gene editing toolbox to include transcription activator-like (TAL) effector nuclease (TALEN)-and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-stimulated homology-directed repair (HDR) using plasmid, rAAV, and oligonucleotide templates. Toward the genetic dehorning of dairy cattle, we introgressed a bovine POLLED allele into horned bull fibroblasts. Single nucleotide alterations or small indels were introduced into 14 additional genes in pig, goat, and cattle fibroblasts using TALEN mRNA and oligonucleotide transfection with efficiencies of 10-50% in populations. Several of the chosen edits mimic naturally occurring performance-enhancing or disease-resistance alleles, including alteration of single base pairs. Up to 70% of the fibroblast colonies propagated without selection harbored the intended edits, of which more than onehalf were homozygous. Edited fibroblasts were used to generate pigs with knockout alleles in the DAZL and APC genes to model infertility and colon cancer. Our methods enable unprecedented meiosis-free intraspecific and interspecific introgression of select alleles in livestock for agricultural and biomedical applications.

show abstract

Bovine Polledness – An Autosomal Dominant Trait with Allelic Heterogeneity

Cited by 126 publications

References 38 publications

The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin

The 80-kb DNA duplication on BTA1 is the only remaining candidate mutation for the polled phenotype of Friesian origin

On the History of Cattle Genetic Resources

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

Contact Info

Product

Resources

About