Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells

Sudaryatma, Putu Eka; Nakamura, Kimika; Mekata, Hirohisa; Sekiguchi, Satoshi; Kubo, Meiko; Kobayashi, Ichiro; Subangkit, Mawar; Goto, Yoshitaka; Okabayashi, Tamaki

doi:10.1016/j.vetmic.2018.04.031

Cited by 30 publications

(27 citation statements)

References 28 publications

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“…BRSV was inactivated by treatment with ultraviolet light for 1 h (UV-inactivated BRSV). The inactivation was confirmed by plaque assay for measuring infectivity (Sudaryatma et al, 2018).…”

Section: Virus and Bacteriamentioning

confidence: 98%

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

Sudaryatma

Saito

Mekata

et al. 2020

Veterinary Microbiology

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The synergistic infection of bovine respiratory syncytial virus (BRSV) and Pasteurella multocida (PM) may predispose cattle to develop severe pneumonia. Previously, we reported that BRSV infection significantly decreased PM adherence to the upper respiratory epithelial cells. It may allow bacteria to invade into the lower respiratory tract and lead to severe pneumonia. To investigate whether BRSV infection regulates the cell surface adherence receptor on bovine trachea epithelial cells (bTECs), we performed proteomic and functional analyses. BRSV infection decreased the expression of intercellular adhesion molecule-1 (ICAM1) on bTECs. Inhibition and knockdown experiments using anti-ICAM1 antibody and siRNAs targeting ICAM1 indicated that PM adherence to bTECs was dependent on ICAM1 expression. These data suggest that under normal conditions bTECs may capture PM in the upper respiratory tract, while BRSV infection reverses this mechanism. The proposed gateway function of bTECs is disrupted by BRSV infection that may facilitate bacterial invasion into the lower respiratory tract and lead to secondary or more severe respiratory infection.

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“…BRSV was inactivated by treatment with ultraviolet light for 1 h (UV-inactivated BRSV). The inactivation was confirmed by plaque assay for measuring infectivity (Sudaryatma et al, 2018).…”

Section: Virus and Bacteriamentioning

confidence: 98%

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

Sudaryatma

Saito

Mekata

et al. 2020

Veterinary Microbiology

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“…3,20 Similar results also have been shown after coinfection of bovine bronchial epithelial cells with BRSV and P multocida. 21…”

Section: Airway and Lung Epithelia And Resistance To Bovine Respiratomentioning

confidence: 99%

The Immunology of Bovine Respiratory Disease

McGill

Sacco

2020

Veterinary Clinics of North America: Food Animal Practice

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“…A BRSV inoculum that would generate multiplicity of infection (MOI) 1 was subjected to ultraviolet light illumination for 1 h to inactivate the virus (UV-BRSV). The inactivation was confirmed by plaque assay as described previously (Sudaryatma et al, 2018).…”

Section: Virus and Bacteriummentioning

confidence: 99%

Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

et al. 2020

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Coinfection by bovine respiratory syncytial virus (BRSV) and Pasteurella multocida (PM) frequently has been observed in cattle that develop severe pneumonia. We recently reported that BRSV infection significantly increased PM adherence to bovine lower respiratory tract epithelial cells. However, the molecular mechanisms of enhanced PM adherence are not completely understood. To investigate whether BRSV infection regulates any cellular adherence receptors on bovine bronchus-and lung-epithelial cells, we performed proteomic and functional analyses. The proteomic analysis showed that BRSV infection increased the accumulation of the platelet-activating factor receptor (PAFR) in both cell types. Molecular experiments, including specific blockade, knockdown, and overexpression of PAFR, indicated that PM adherence to these cell types depended on PAFR expression. These findings highlight the role, in cattle with severe pneumonia, of the synergistic effect of coinfection by BRSV and PM in the lower respiratory tract.

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Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells

Cited by 30 publications

References 28 publications

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

The Immunology of Bovine Respiratory Disease

Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

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