Bovine rotavirus G and P types and sequence analysis of the VP7 gene of two G8 bovine rotaviruses from Japan

Genetic and Serological Characterization of Novel Serotype G8 Bovine Group A Rotavirus Strains Isolated in Japan

“…The majority of BoRV-A strains were placed in the G8a lineage, only Cody-I801 placed with the G8b lineage, and the majority of human RV-A strains were classified as either G8c or G8d lineages. Okada et al reported that Tokushima9503 and Niigata9801 clustered together and formed a new group in the phylogenetic analysis of the VP7 gene sequences [19]. In this study, the five isolates placed in the G8a lineage, together with BRV16, Tokushima9503 and Niigata9801.…”

“…Although our findings suggest that G8 BoRV-A should be investigated in detail, little investigation of G8 BoRV-A has been carried out to date because all G8 strains reported so far have been detected and/or isolated only sporadically [4,19,20,22,23,26]. Even in Japan, there are few reports on the characterization of G8 BoRV-A [19,23]. In this study, G8 BoRV-A strains recovered from five calves in Kagoshima Prefecture, Japan were genetically and serologically characterized by sequence analysis of the gene encoding the neutralization protein VP7 and virus neutralization studies.…”

“…Our studies were the first to describe G8 as the most predominant BoRV-A strain during the research period. Although our findings suggest that G8 BoRV-A should be investigated in detail, little investigation of G8 BoRV-A has been carried out to date because all G8 strains reported so far have been detected and/or isolated only sporadically [4,19,20,22,23,26]. Even in Japan, there are few reports on the characterization of G8 BoRV-A [19,23].…”

“…Of these, the following five isolates from calves raised in five different herds were characterized in this study: KAG74, KAG75, KAG80, KAG87 and KAG91. BoRV-A NCDV (G6, P6 [1]) [17], UK (G6, P7 [5]) [28], BRV16 (G8, P6 [1]) [23], Tokushima9503 (G8, P [11]) [19], Niigata9801 (G8, P [14]-like) [19] and KK3 (G10, P8 [11]) [18] strains were used as reference strains. The isolated strains and reference strains were propagated and plaque-purified three times in MA-104 cells in the presence of trypsin [16].…”

Genetic diversity in three bovine-like human G8P[14] and G10P[14] rotaviruses suggests independent interspecies transmission events

“…The P[14] RVA genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission as it has been found in RVA strains from several distinct animal species, such as rabbits, cows, goats, sheep and guanaco. P [14] strains are found most commonly in combination with G3 or the typical bovine-like G-genotypes: G6, G8 and to a lesser extent G10 (Chitambar et al, 2011;Esona et al, 2009;Okada & Matsumoto, 2002;Parreño et al, 2004).…”