Bovine Serum Albumin Adsorption on Hydroxyapatite and Biphasic Calcium Phosphate and the Correlation with Zeta Potentials and Wettability

Abstract

Studying on the interaction between proteins and calcium phosphate implants is one of the basic subjects in biomaterials science and engineering. In this work, zeta potentials and contact angles of hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramics were measured, and the adsorption behaviors of bovine serum albumin (BSA) on the surfaces were investigated. The adsorption isotherms of BSA on both ceramics follow the Langmuir type, however, BCP shows higher BSA adsorption ability. The differences of contact angles and zeta potentials in HA and BCP are the key factors to determine their ability to bind BSA molecules.

Effect of phase composition and microstructure of calcium phosphate ceramic particles on protein adsorption

“…Therefore, HA particles always have a lower microporosity than BCP particles. Previous reports have shown that the adsorption isotherms for BSA on HA and BCP particles were of the Langmuir type [33][34][35]. In the present study, according to Figs.…”

“…It is known that protein adsorption on a solid surface is a quite complex process, and one of the main driving forces is electrostatic interaction between the material and the protein [1,4]. Both HA and BCP have negative surface f potentials in PBS, pH 7.4 [35], and the isoelectric points (IEP) of collagen, fibrinogen and insulin are $4.5 [36], 5.8 [37] and 5.3 [38], respectively. In PBS all of the three model proteins have negative surface net charges, resulting in electrostatic repulsion between the CaP powders and the proteins.…”

A thermostability perspective on enhancing physicochemical and cytological characteristics of octacalcium phosphate by doping iron and strontium

“…BSA and LSZ were selected as the model proteins for their distinctly different electrostatic properties. BSA is a flexible globular macromolecule (Mw ~66 kDa) with an isoelectric point of 4.7 [ 41 ]. BSA adsorption is generally driven by its conformational change and the electrostatic interaction of surface-molecule [ 41 ].…”

“…BSA is a flexible globular macromolecule (Mw ~66 kDa) with an isoelectric point of 4.7 [ 41 ]. BSA adsorption is generally driven by its conformational change and the electrostatic interaction of surface-molecule [ 41 ]. For undoped OCP, compared to the untreated samples, the preponderant BSA adsorption on the heat-treated samples could be mainly due to the lower electrostatic repulsions of surface-BSA and the conformation changes of BSA molecules on the surface based on the result of specific surface area normalization (see Fig.…”

Bovine Serum Albumin Adsorption onto Hydroxyapatite and Biphasic Calcium Phosphate Ceramic Granules

“…Proteins act as contact for cell attachment, therefore their adsorption on implant material is important for bonding the biomaterial to the bony tissue [4]. The most abundant protein in the circulatory system is bovine serum albumin (BSA); therefore, this protein is mostly used in vitro studies in order to evaluate the bioactivity of calcium phosphate ceramic powders [4,[5][6][7] and disks [6]. In most of these studies pure or modified HAp is used.…”