Bovine serum albumin enhances calcium currents in chicken granulosa cells

Chiang, Meicheng; Strong, Judith A.; Asem, Elikplimi K.

doi:10.1016/0303-7207(93)90048-o

Cited by 14 publications

(5 citation statements)

References 47 publications

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“…The present results confirm an earlier report that chicken granulosa cells express T-and L-type voltage-dependent Ca 2ϩ channels (58). The present results also confirm reports that chicken (14,45,57,58,65) and porcine (38) granulosa cells express whole cell T-type Ca 2ϩ currents.…”

Section: Discussionsupporting

confidence: 92%

“…Experiments with radioisotopes demonstrated transmembrane Ca 2ϩ exchange in porcine granulosa cells (62), and studies with Ca 2ϩ -sensitive fluorescent dyes revealed Ca 2ϩ influx into rat (66), chicken (32,49), and porcine (23-25) granulosa cells. Furthermore, experiments conducted with patch clamp techniques showed that avian and mammalian granulosa cells express Ca 2ϩ -specific channels (57) and Ca 2ϩ currents (14,38,58,65).…”

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confidence: 99%

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Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Asem¹,

Qin²,

Rane

2002

American Journal of Physiology-Endocrinology and Metabolism

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Patch clamp experiments were conducted to study the effects of basal lamina (basement membrane) of chicken ovarian follicle on membrane Ca(2+) currents in differentiated chicken granulosa cells in a homologous system. The whole cell patch clamp technique was used to simultaneously monitor membrane capacitance (an indirect measure of total cell surface area) and currents flowing through voltage-dependent Ca(2+) channels (using Ba(2+) as the charge carrier). Membrane capacitance was smaller in cells incubated on intact basal lamina than in control cells (incubated on tissue culture-treated plastic substratum). Granulosa cells expressed both T- and L-type Ca(2+) currents, and the amplitudes of the currents in cells incubated on intact basal lamina were significantly lower than those of control cells. Also, granulosa cells incubated on intact basal lamina were found to have significantly lower T- or L-type Ca(2+) current densities than control cells. Intact basal lamina that had been stored for 12 mo produced effects on T- and L-type Ca(2+) currents similar to those caused by freshly isolated basal lamina. The basal lamina was solubilized completely in one step and used to coat glass coverslips (uncoated glass coverslips served as controls). Granulosa cells incubated on coverslips precoated with solubilized basal lamina assumed spherical shape similar to those incubated on intact basal lamina. Similar to the observations made for intact basal lamina, the solubilized basal lamina suppressed T- and L-type Ca(2+) currents in the differentiated granulosa cells. Moreover, fibronectin, laminin, and type IV collagen, obtained from commercial sources, attenuated T- and L-type Ca(2+) currents in the differentiated granulosa cells. This interplay between basal lamina and Ca(2+) currents may be one mechanism that subserves the effects of the matrix material on metabolic functions of granulosa cells.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 99%

Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Asem¹,

Qin²,

Rane

2002

American Journal of Physiology-Endocrinology and Metabolism

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“…Results of the present study confirm the earlier report of Mealing et al (36) that differentiated chicken granulosa cells express an inward Ca 2ϩ -dependent Cl Ϫ whole cell current. The present results also confirm the earlier observation that differentiated chicken granulosa cells express a transient inward current characteristic of T-type Ca 2ϩ current (16,36,49). Furthermore, the outward current observed in the present study has the characteristics of previously described delayed-rectifier K ϩ current in chicken (16,43,46) and swine (29) granulosa cells.…”

Section: Discussionsupporting

confidence: 92%

“…In addition, changes in cytosolic levels of monovalent (K ϩ and Na ϩ ) and divalent (Ca 2ϩ ) cations are critical events in the regulation of the functions of the ovarian granulosa cell (31). It was demonstrated that inorganic ions enter or leave granulosa cells through transmembrane channels (6,16,17,29,35,36,45,46) or are carried by nonchannel transport proteins (9,32,33).…”

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Basal lamina of ovarian follicle regulates an inward Cl⁻ current in differentiated granulosa cells

Qin¹,

Rane

Asem³

2002

American Journal of Physiology-Cell Physiology

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Patch-clamp experiments were conducted to study the effects of basal lamina (basement membrane) of preovulatory chicken ovarian follicle on membrane currents in differentiated chicken granulosa cells in a homologous system. The membrane capacitance (measure of total membrane area) was smaller in cells cultured on intact basal lamina than that of control cells. The granulosa cells expressed outward and two inward currents. A small fraction of the cells (3%) expressed only a transient fast-activating and -inactivating inward current carried by Ca(2+). The majority of the cells, however, expressed a slowly activating and inactivating inward current (carried by Cl(-)) that was superimposed on the transient Ca(2+) current. All cells expressed an outward current characteristic of the delayed-rectifier K(+) current. The removal of extracellular Ca(2+) led to elimination of the slow inward Cl(-) current, indicating that it is a Ca(2+)-dependent Cl(-) current. Both peak amplitude and current density of the inward Cl(-) current were significantly lower in cells cultured on freshly isolated intact basal lamina (or basal lamina stored at 4 degrees C for 12 mo) than those of control cells; however, basal lamina had no significant effect on the density of the outward current. Similar to the observations made for intact basal lamina, solubilized basal lamina suppressed the inward Cl(-) current in differentiated granulosa cells. These data show that homologous basal lamina modulates a Ca(2+)-dependent Cl(-) current in differentiated granulosa cells. These findings provide a partial explanation for the mechanisms that subserve the reported effects of basal lamina (basement membrane) on the metabolic functions of differentiated granulosa cells.

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“…Another possible interpretation of the effects of BSA on the Ca 2+ -release channel takes into consideration recent reports describing an increase in voltage activated Ca 2+ current after application of BSA (Chiang et al 1993, Smith & Meves 1994. This effect was preferentially attributed to a change of surface charge density due to the adsorption of BSA to the membrane.…”

Section: Discussionmentioning

confidence: 99%

Effect of bovine serum albumin on the calcium release channel of sarcoplasmic reticulum from rabbit skeletal muscle

Catinot

Bastide

Montel

et al. 1997

Acta Physiologica Scandinavica

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The effect of bovine serum albumin (BSA) on the activity of the calcium release channel of the sarcoplasmic reticulum from rabbit skeletal muscle was investigated using both tension recording from skinned fibres and electrophysiological recording of unitary channel currents from planar lipid membranes. BSA had no effect on the Ca2+ affinity of the contractile proteins, elicited no tension per se in Ca2+‐loaded skinned fibres, but potentiated caffeine‐induced tension. Maximum potentiation was observed with 0.05–0.5% BSA. BSA (0.1%) had no detectable effect on the basal activity of the Ca2+‐release channel incorporated in lipid bilayer. However, channel stimulation elicited by either caffeine (2 mM) or ATP (60 μM) was further enhanced by BSA (0.1%), as indicated by significant increases in Po, the open probability of the channel. These results suggest that BSA can modulate the response of the skeletal muscle SR Ca2+‐release channel to different activators such as caffeine and ATP.

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Bovine serum albumin enhances calcium currents in chicken granulosa cells

Cited by 14 publications

References 47 publications

Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Basal lamina of ovarian follicle regulates an inward Cl⁻ current in differentiated granulosa cells

Effect of bovine serum albumin on the calcium release channel of sarcoplasmic reticulum from rabbit skeletal muscle

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Bovine serum albumin enhances calcium currents in chicken granulosa cells

Cited by 14 publications

References 47 publications

Effect of basal lamina of ovarian follicle on T- and L-type Ca2+currents in differentiated granulosa cells

Effect of basal lamina of ovarian follicle on T- and L-type Ca2+currents in differentiated granulosa cells

Basal lamina of ovarian follicle regulates an inward Cl− current in differentiated granulosa cells

Effect of bovine serum albumin on the calcium release channel of sarcoplasmic reticulum from rabbit skeletal muscle

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Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Effect of basal lamina of ovarian follicle on T- and L-type Ca²⁺currents in differentiated granulosa cells

Basal lamina of ovarian follicle regulates an inward Cl⁻ current in differentiated granulosa cells