Bovine Staphylococcus aureus: Association of virulence genes, genotypes and clinical outcome

Fournier, C.; Kuhnert, Peter; Frey, Joachim; Miserez, R.; Kirchhofer, M.; Kaufmann, Thomas; Steiner, Adrian; Graber, H.U.

doi:10.1016/j.rvsc.2008.01.010

Cited by 128 publications

(267 citation statements)

References 33 publications

Supporting

Mentioning

232

Contrasting

Unclassified

Order By: Relevance

“…Distribution of genotypes among isolates originated in different geographical areas is shown in Table 1 (4,7); however associations in these latter cases were established with patterns including several rather than individual genes (4).…”

Section: Capsular Polysaccharides In Vitro Expression Does Notmentioning

confidence: 99%

Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina

Camussone¹,

Rejf²,

Pujato³

et al. 2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

Section: Capsular Polysaccharides In Vitro Expression Does Notmentioning

confidence: 99%

Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina

Camussone¹,

Rejf²,

Pujato³

et al. 2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…aureus involved in bovine mastitis (Fournier et al, 2008;Graber et al, 2009). Fournier et al (2008) developed a genotyping method based on amplifying the 16S-23S rRNA intergenic spacer region by PCR (ribosomal spacer-PCR, RS-PCR), which showed the associations of genotype with virulence gene pattern and clinical properties of the disease. In the first instance, 17 different genotypes were found and further characterized for their virulence gene patterns (Fournier et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Fournier et al (2008) developed a genotyping method based on amplifying the 16S-23S rRNA intergenic spacer region by PCR (ribosomal spacer-PCR, RS-PCR), which showed the associations of genotype with virulence gene pattern and clinical properties of the disease. In the first instance, 17 different genotypes were found and further characterized for their virulence gene patterns (Fournier et al, 2008). Staphylococcus aureus genotype B (GTB) and GTC were the most frequent genotypes found in bovine milk (80%), but only GTB was contagious, with cow prevalences of up to 87% within herds, whereas GTC showed noncontagious behavior, infecting only single cows in a herd and single quarters of the udder (Fournier et al, 2008;Graber et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In the first instance, 17 different genotypes were found and further characterized for their virulence gene patterns (Fournier et al, 2008). Staphylococcus aureus genotype B (GTB) and GTC were the most frequent genotypes found in bovine milk (80%), but only GTB was contagious, with cow prevalences of up to 87% within herds, whereas GTC showed noncontagious behavior, infecting only single cows in a herd and single quarters of the udder (Fournier et al, 2008;Graber et al, 2009). Staphylococcus aureus GTB is characterized by the presence of the enterotoxin genes sea, sed, and sej and a SNP within the lukE gene (lukEB).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer

Boss

Cosandey

Luini

et al. 2016

Journal of Dairy Science

Self Cite

View full text Add to dashboard Cite

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies using ribosomal spacer (RS)-PCR, however, demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infections are genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. Furthermore, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. In addition to RS-PCR, other methods for subtyping Staph. aureus are known, including spa typing and multilocus sequence typing (MLST). They are based on sequencing the spa and various housekeeping genes, respectively. The aim of the present study was to compare the 3 analytic methods using 456 strains of Staph. aureus isolated from milk of bovine intramammary infections and bulk tanks obtained from 12 European countries. Furthermore, the phylogeny of animal Staph. aureus was inferred and the zoonotic transfer of Staph. aureus between cattle and humans was studied. The analyzed strains could be grouped into 6 genotypic clusters, with CLB, CLC, and CLR being the most prominent ones. Comparing the 3 subtyping methods, RS-PCR showed the highest resolution, followed by spa typing and MLST. We found associations among the methods but in many cases they were unsatisfactory except for CLB and CLC. Cluster CLB was positive for clonal complex (CC)8 in 99% of the cases and typically positive for t2953; it is the cattle-adapted form of CC8. Cluster CLC was always positive for t529 and typically positive for CC705. For CLR and the remaining subtypes, links among the 3 methods were generally poor. Bovine Staph. aureus is highly clonal and a few clones predominate. Animal Staph. aureus always evolve from human strains, such that every human strain may be the ancestor of a novel animal-adapted strain. The zoonotic transfer of IMI-and milk-associated strains of Staph. aureus between cattle and humans seems to be very limited and different hosts are not considered as a source for mutual, spontaneous infections. Spillover events, however, may happen.

show abstract

“…Seven of the eight isolates were positive for the enterotoxin sea, which is a common finding in S. aureus genotype B (GTB) strains (Fournier et al, 2008;Graber et al, 2009). Furthermore, S. aureus isolates belonging to clonal complex 8 and spa type t2953 are a common finding in mastitis milk in Swiss dairy herds as described by Johler et al (2011).…”

Section: Discussionmentioning

confidence: 99%

Success of interventions in mastitis problems with Staphylococcus aureus after the introduction of an automatic milking system

Ruf¹,

Johler²,

Merz³

et al. 2015

SAT

View full text Add to dashboard Cite

Staphylococcus aureus (S. aureus) is often the cause of mastitis problems in dairy herds and causes great economic losses. In this study, isolates from a dairy herd with a known S. aureus mastitis problem were examined by means of molecular methods (spa typing, PFGE, and DNA microarray) to investigate their epidemiological relationship and the success of intervention measures. The investigated dairy farm has a herd size of 60 cows and uses a fully automated milking system for milk production. A S. aureus strain, which contaminated the automated milking system and was subsequently spread among the herd through the latter, was suspected to be the origin of the mastitis problem within the herd. Thanks to the applied molecular methods, the common origin of the S. aureus isolates from the collected milk and swab samples could be shown. By culling chronically infected cows, optimising dry cow management and ensuring reliable intermediate cluster disinfection, the bulk milk somatic cell count improved

show abstract

Bovine Staphylococcus aureus: Association of virulence genes, genotypes and clinical outcome

Cited by 128 publications

References 33 publications

Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina

Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina

Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer

Success of interventions in mastitis problems with Staphylococcus aureus after the introduction of an automatic milking system

Contact Info

Product

Resources

About