Boxwood Blight: Threat to Ornamentals

Daughtrey, Margery

doi:10.1146/annurev-phyto-082718-100156

Cited by 66 publications

(89 citation statements)

References 60 publications

(103 reference statements)

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“…This study began with collecting and air-drying diseased leaves then storing them in a cold room at 4 • C for 15 months. One hundred stored leaves were spread on the surface of potting mix under healthy Justin Brouwers plants in a container, then immediately cover sprayed (not onto the boxwood foliage) with a mixture of 35 mL NB culture of SSG and 35 mL SDW at a final concentration of 10 8 cfu/mL or 1 2 strength NB without SSG. Each treatment included three replicate plant containers and treatments were arranged in a randomized complete block design.…”

Section: Ssg Effect On Potential Of Diseased Leaves As a Source Of Inmentioning

confidence: 99%

“…Boxwood blight caused by Calonectria pseudonaviculata (Cps) is an economically and ecologically important disease [1,2]. This disease was first reported in New Zealand [3] and the United Kingdom [4].…”

Section: Introductionmentioning

confidence: 99%

“…This disease was first reported in New Zealand [3] and the United Kingdom [4]. In the United States, boxwood blight was first confirmed in Connecticut and North Carolina [5], and now it has been reported in 28 states [1,6]. Cps attacks Buxus, a genus of slow-growing evergreen shrubs including English boxwood (Buxus sempervirens 'Suffruticosa'), an iconic landscape plant in many public and private gardens [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Boxwood blight has rampaged in Europe [20] and now in North America due to limited control options [1]. First, no boxwood cultivar is immune to the blight disease although some cultivars are more tolerant than others [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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A Potent Burkholderia Endophyte against Boxwood Blight Caused by Calonectria pseudonaviculata

Kong

Hong

2020

Microorganisms

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Calonectria pseudonaviculata (Cps) poses an increasing threat to boxwood, a major nursery crop and iconic landscape plant worldwide. Here, we report on a potent biocontrol agent that produces small sage green (SSG) colonies on potato dextrose agar. SSG is a bacterial strain recovered from Justin Brouwers boxwood leaves with unusual response to Cps inoculation. Water-soaked symptoms developed on leaves 2 days after inoculation then disappeared a few days later. This endophyte affected several major steps of the boxwood blight disease cycle. SSG at 107 cfu/mL lysed all conidia in mixed broth culture. SSG at 108 cfu/mL reduced blight incidence by >98% when applied one day before or 3 h after boxwood were inoculated with Cps. Its control efficacy decreased with decreasing bacterial concentration to 103 cfu/mL and increasing lead time up to 20 days. When applied on diseased leaf litter under boxwood plants, SSG reduced Cps sporulation and consequently mitigated blight incidence by 90%. SSG was identified as a new member of the Burkholderia cepacia complex with distinct characters from known clinical strains. With these protective, curative, and sanitizing properties, this Burkholderia endophyte offers great promise for sustainable blight management at production and in the landscape.

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Section: Ssg Effect On Potential Of Diseased Leaves As a Source Of Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Potent Burkholderia Endophyte against Boxwood Blight Caused by Calonectria pseudonaviculata

Kong

Hong

2020

Microorganisms

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“…Symptoms develop more rapidly on highly susceptible cultivars, on younger plant tissues, or when environmental conditions are optimal for rapid infection (warm and moist). Leaf symptoms may be confused with those caused by chemical burns or winter damage, which may have a similar appearance but typically do not lead to defoliation (Daughtrey 2019).…”

Section: Symptoms and Signsmentioning

confidence: 99%

Boxwood Blight Disease: A Diagnostic Guide

Castroagudín

Yang

Daughtrey

et al. 2020

Plant Health Progress

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Boxwood blight, caused by Calonectria henricotiae and C. pseudonaviculata, is an extremely destructive disease of cultivated and native Buxus species (boxwood) worldwide. The disease is widespread in North America, Europe, and Southwest Asia. Early and accurate diagnosis of boxwood blight is the most effective tool used to prevent spread of the disease, and it is the first step for development and implementation of appropriate control measures. This diagnostic guide provides up-to-date information on how to diagnose and identify boxwood blight and also offers an overview of methods used to isolate the pathogen, store cultures, and evaluate pathogenicity of the causal agents.

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First report of Calonectria henricotiae causing box blight in Spain

Kong,

Ioos,

Hong

2024

New Disease Reports

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Box blight is caused by two closely related fungi, Calonectria pseudonaviculata (Cps) and/or C. henricotiae (Che) (Gehesquière et al., 2016). Cps is widespread globally (Daughtrey, 2019) while Che is limited to eight central European countries (Hong, 2023). To determine whether Che is present in southern Europe, we visited ten large boxwood gardens and street plantings in Madrid and Toledo, Spain and in Bordeaux, Lyon and Sainte-Foy-la-Grande, France. Boxwood (Buxus spp.) twigs with black streaks (Figure 1) were only observed in Madrid, Toledo, and Sainte-Foy-la-Grande. Diseased twigs were collected in sealed triple bags and brought to the laboratory under the USDA permit (P526P-21-05960). After surface sterilization with 10% bleach for two minutes and three rinses in deionised water, twigs were placed on a mesh with wet paper towels underneath in closed plastic containers at 23 • C and wetted with sterile distilled water every two days until white mould appeared on leaves (Figure 2). The mould was suspended in 15 µL sterile distilled water then transferred onto a glass slide for microscopy. Following observations of rod-shaped conidia, typical of Calonectria spp., 1-µL drops of the suspensions were pipetted from slides onto acidified potato dextrose agar (PDA) for isolation (Kong et al., 2023). Clean colonies were only obtained from the Madrid and Sainte-Foy-la-Grande samples (Figure 3) and were subcultured on regular PDA. Colony PCR (Kong et al., 2005) was performed from a total of 159 colonies grown from three-day PDA cultures, using Cpsand Chespecific primers (Guo & Pooler, 2021). Also included in the PCR were three French isolates from the ANSES collection: LSVM0960 collected by Department Île-de-France in 2013, LSVM1007 by Department This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Boxwood Blight: Threat to Ornamentals

Cited by 66 publications

References 60 publications

A Potent Burkholderia Endophyte against Boxwood Blight Caused by Calonectria pseudonaviculata

A Potent Burkholderia Endophyte against Boxwood Blight Caused by Calonectria pseudonaviculata

Boxwood Blight Disease: A Diagnostic Guide

First report of Calonectria henricotiae causing box blight in Spain

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