BPA exposure during in vitro oocyte maturation results in dose-dependent alterations to embryo development rates, apoptosis rate, sex ratio and gene expression

Ferris, Jacqueline; Mahboubi, Kiana; MacLusky, Neil J.; King, W. A.; Favetta, Laura A.

doi:10.1016/j.reprotox.2015.12.002

Cited by 57 publications

(41 citation statements)

References 66 publications

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“…These data suggest a BPS-mediated effect on gamete quality [29]. Concerning BPA, treatment during IVM in bovines impairs embryo development [55]. A similar phenomenon occurs in Xenopus laevis [56].…”

Section: Low Bps Doses Impaired In Vitro Ovine Early Developmental Oomentioning

confidence: 56%

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Desmarchais

Têteau

Papillier

et al. 2020

IJMS

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Introduction: Bisphenol A (BPA) is a widespread compound in the plastic industry that is especially used to produce baby bottles, food packaging and metal cans. BPA, an endocrine disruptor, leads to alterations in reproductive function and therefore has been banned from the food industry. Unregulated BPA analogues, particularly Bisphenol S (BPS), have emerged and are now used in the plastic industry. Thus, this study aimed to examine the acute effects of low and environmental doses of BPS on ewe oocyte quality and developmental competence, and its mechanism of action, during in vitro maturation. Methods: Ewe cumulus-oocyte complexes underwent in vitro maturation in the presence or absence of BPS (1 nM, 10 nM, 100 nM, 1 µM or 10 µM). Oocytes were then subjected to in vitro fertilisation and development. Results: 1 µM BPS induced a 12.7% decrease in the cleavage rate (p = 0.004) and a 42.6% decrease in the blastocyst rate (p = 0.017) compared to control. The blastocyst rate reduction was also observed with 10 nM BPS. Furthermore, 10 µM BPS reduced the oocyte maturation rate, and 1 µM BPS decreased cumulus cell progesterone secretion. PR and AMH gene expression were reduced in cumulus cells. BPS induced a 5-fold increase in MAPK 3/1 activation (p = 0.04). Conclusions: BPS impaired ewe oocyte developmental competence. The data suggest that BPS might not be a safe BPA analogue. Further studies are required to elucidate its detailed mechanism of action.

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Section: Low Bps Doses Impaired In Vitro Ovine Early Developmental Oomentioning

confidence: 56%

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Desmarchais

Têteau

Papillier

et al. 2020

IJMS

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“…The ability of BPS to regulate mRNA expression was previously confirmed only in somatic cells 37 . BPA alters the global supply of gene transcripts connected to key cell processes in oocytes 38 . Altered signalling during processes leading to destabilization of the overall maternal stock of mRNA in oocytes 39 or its selective degradation 40 may be responsible for the decrease in mRNA transcript levels that we observed.…”

Section: Discussionmentioning

confidence: 99%

Bisphenol S negatively affects the meotic maturation of pig oocytes

Žalmanová¹,

Hošková²,

Nevoral³

et al. 2017

Sci Rep

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Bisphenol A (BPA), a chemical component of plastics, is a widely distributed environmental pollutant and contaminant of water, air, and food that negatively impacts human health. Concerns regarding BPA have led to the use of BPA-free alternatives, one of which is bisphenol S (BPS). However, the effects of BPS are not well characterized, and its specific effects on reproduction and fertility remain unknown. It is therefore necessary to evaluate any effects of BPS on mammalian oocytes. The present study is the first to demonstrate the markedly negative effects of BPS on pig oocyte maturation in vitro, even at doses lower than those humans are exposed to in the environment. Our results demonstrate (1) an effect of BPS on the course of the meiotic cell cycle; (2) the failure of tubulin fibre formation, which controls proper chromosome movement; (3) changes in the supply of maternal mRNA; (4) changes in the protein amounts and distribution of oestrogen receptors α and β and of aromatase; and (5) disrupted cumulus cell expansion. Thus, these results confirm that BPS is an example of regrettable substitution because this substance exerts similar or even worse negative effects than those of the material it replaced.

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“…The intracellular apoptosis level of blastocysts was measured in a TUNEL assay using an In Situ Cell Death Detection Kit (11684795910; Roche, Basel, Switzerland) as previously described by Ferris et al (21). After washing 3 times with PBS and PVA, blastocysts were fixed in 3.7% paraformaldehyde for 30 min at room temperature and subsequently permeabilized by incubation in 0.5% Triton X-100 for 30 min at room temperature.…”

Section: Tunel Assaymentioning

confidence: 99%

PINK1 regulates mitochondrial morphologyviapromoting mitochondrial fission in porcine preimplantation embryos

et al. 2019

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Phosphatase and tensin homolog–induced kinase 1 (PINK1) on the outer membranes of impaired mitochondria promotes mitophagy and regulates mitochondrial morphology. Mammalian oocytes and early embryos are mitochondria rich, but mitochondrial dynamics during preimplantation embryo development is not well‐studied. To investigate whether PINK1 is required for mitochondrial dynamics in porcine preimplantation embryos, gene knockdown and inhibitors were used, and mitochondrial dynamics were observed by transmission electron microscopy. PINK1 knockdown significantly impaired blastocyst formation and quality, induced mitochondrial elongation and swelling, and reduced mitochondrial DNA copy number. PINK1 knockdown–induced mitochondrial elongation caused mitochondrial dysfunction, oxidative stress, and ATP deficiency, significantly increasing autophagy and apoptosis. Profission dynamin‐related protein 1 overexpression prevented PINK1 knockdown–induced impairment of embryo development, mitochondrial elongation, and dysfunction. Thus, PINK1 promotes mitochondrial fission in porcine preimplantation embryos.—Niu, Y.‐J., Nie, Z.‐W., Shin, K.‐T., Zhou, W., Cui, X.‐S. PINK1 regulates mitochondrial morphology via promoting mitochondrial fission in porcine preimplantation embryos. FASEB J. 33, 7882–7895 (2019). http://www.fasebj.org

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BPA exposure during in vitro oocyte maturation results in dose-dependent alterations to embryo development rates, apoptosis rate, sex ratio and gene expression

Cited by 57 publications

References 66 publications

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Bisphenol S negatively affects the meotic maturation of pig oocytes

PINK1 regulates mitochondrial morphologyviapromoting mitochondrial fission in porcine preimplantation embryos

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