Bradford quantification of Glomalin-Related Soil Protein in coloured extracts of forest soils

Cissé, Gaoussou; Essi, M.; Nicolas, Manuel; Staunton, Siobhán

doi:10.1016/j.geoderma.2020.114394

Cited by 14 publications

(9 citation statements)

References 11 publications

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“…To avoid the problems from co-extraction of humic substances, we applied a colour correction as in (Cissé et al, 2020;Moragues-Saitua et al, 2018). A subsample of 1.25 g of dry soil was sieved to 0.5 mm, and 10 mL (1:8 soil: extract) sodium citrate (20 mM) was added and the mixture was adjusted to pH 7 with HCl.…”

Section: Analysis Methods For Soil Nitrogen Poolsmentioning

confidence: 99%

“…The autoclave citrate‐extracted protein (ACE protein) was analysed with readily available equipment (microplates, autoclave, centrifuge, Bradford protein assay) but with a highly customized protocol (Hurisso et al, 2018). To avoid the problems from co‐extraction of humic substances, we applied a colour correction as in (Cissé et al, 2020; Moragues‐Saitua et al, 2018). A subsample of 1.25 g of dry soil was sieved to 0.5 mm, and 10 mL (1:8 soil: extract) sodium citrate (20 mM) was added and the mixture was adjusted to pH 7 with HCl.…”

Section: Methodsmentioning

confidence: 99%

“…This corresponded to the “easily extractable glomalin related soil protein” pool (Wright & Upadhyaya, 1996), which consists of proteins that are extracted by the citrate and not denatured by the autoclave. To avoid overestimation of the protein amount by the spectrophotometric method in the Bradford assay, the colour correction by (Cissé et al, 2020) was used. A sample blank was used, which included the extracted sample, but not the Bradford reagent and was adjusted to the same pH as the Bradford reagent added sample.…”

Section: Methodsmentioning

confidence: 99%

“…To avoid double counting the well, a buffer‐filled well was included and added to the measurement (i.e. total measurement A protein = A bradford − A coloured + well + A well ) (Cissé et al, 2020). If the absorbance was too high compared with the standards (bovine serum albumin, BSA 0–350 mg/L), the samples were diluted (1:2 or 1:3) to maintain the analysis on the linear standard curve.…”

Section: Methodsmentioning

confidence: 99%

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Do carbon farming practices build bioavailable nitrogen pools?

Mattila,

Girz,

Pihlatie

2023

Soil Use and Management

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Agricultural soils contain large amounts of nitrogen (N), but only a small fraction is readily available to plants. Despite several methods developed to estimate the bioavailability of N, there is no consensus on which extraction methods to use, and which N pools are critically important. In this study, we measured six soil N pools from 20 farms, which were part of a multi‐year soil carbon sequestration on‐farm experiment (Carbon action, 2019–2023). The aim was to quantify the N pools and to evaluate if farming practices that aim to build soil carbon pools, also build bioavailable N pools. We also aimed to test if the smaller and rapidly changing N pools could serve as an indicator for the slower change in soil organic matter. The measured N pools decreased in size, when moving from total N (7700 ± 1500 kg/ha) to slowly cycling (Illinois Soil Nitrogen Test ISNT‐N: 1063 ± 220 kg/ha, autoclave citrate‐extracted ACE protein N: 633 ± 440 kg/ha), water‐soluble organic N (50 ± 17 kg/ha), potentially mineralizable N (33 ± 13 kg/ha) and finally readily plant available inorganic pools (nitrate and ammonium, total: 14 ± 8 kg/ha). In total, the measured pools covered only 18%–44% of total N, indicating a large unidentified N pool, which is either tightly bound to soil mineral fraction and not easily extractable or is bound to undecomposed plant residues and not hydrolysed by the methods. Of the large N pools (ISNT‐N, ACE protein and unidentified residual N), clay, carbon (C) and C:Clay ratios explained most of the variability (R2 = .90–.93), leaving a minor part of the variation to the management effect. A pairwise comparison of carbon farming and control plots concluded that farming practices had a small (3%–5%) but statistically significant (p < .05) effect on soil total N and ISNT‐N pools, and a moderate and significant effect (18%, p < .01) on potentially mineralizable N. The large variation in protein N, water‐soluble organic N and inorganic N reduced statistical significance, although individual C sequestration practices had large effects (−30% to +50%). In conclusion, carbon sequestration practices can build both slowly cycling N pools (ISNT) and increase the mineralisation rate of these pools to release plant available forms, resulting in an additional benefit to agriculture through reduced fertilizer application needs.

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Section: Analysis Methods For Soil Nitrogen Poolsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Do carbon farming practices build bioavailable nitrogen pools?

Mattila,

Girz,

Pihlatie

2023

Soil Use and Management

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“…The possibility of eschewing such interferences was outlined by Redmile‐Gordon et al. (2013) employing the modified Lowry method (Cissé et al., 2020). Despite criticism of glomalin or GRSP, it can be seen as a defined part of the organic matter in the soil with the established relations to mycorrhiza and the soil quality (Rotter et al., 2017; Vasconcellos et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

Alteration in the amount of Glomalin in transition from forest to field/meadow

Vranová

Holík

2020

Soil Use and Management

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Glomalin produced by fungal mycelium represents a significant stabilizing element of soil aggregates and a relatively stable carbon reservoir in the soil. The aim of our research was to investigate the changes in the amount of glomalin‐related soil protein (GRSP) associated with the transition from forest to meadow or field conditions at the four transects during the 2016 growing season. The method Near Infrared Spectrometry was applied for measuring the amount of GRSP. The measurements have demonstrated that the amount of GRSP decreased both in the points adjacent to the ecotone in the meadow and the field at the Bukovinka and the Proklest A transects. At the two transects, the changes in the amount of GRSP between the forest, the ecotone and the meadow were statistically insignificant except for the point 4 where more GRSP was found out in the meadow and the ecotone than in the forest.

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Arbuscular Mycorrhizal Fungi and Higher Plants

2024

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show abstract

Bradford quantification of Glomalin-Related Soil Protein in coloured extracts of forest soils

Cited by 14 publications

References 11 publications

Do carbon farming practices build bioavailable nitrogen pools?

Do carbon farming practices build bioavailable nitrogen pools?

Alteration in the amount of Glomalin in transition from forest to field/meadow

Arbuscular Mycorrhizal Fungi and Higher Plants

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