Bradykinin-evoked Release of [3H]Noradrenaline from the Human Neuroblastoma SH-SY5Y

Road, Leicester LEI 9HN 1 Muscarinic and bradykinin receptor-mediated Ins(1,4,5)P3 accumulation, Ca2+ mobilization and Ca2+ entry have been examined in human SH-SY5Y neuroblastoma cells. This has allowed both direct comparison of signalling events by two receptor types potentially linked to the same transduction pathway and an investigation of the interactions between the components of this pathway. 2 Stimulation of muscarinic receptors with carbachol produced biphasic accumulations of Ins(1,4,5)P3 consisting of a rapid peak followed by a lower sustained phase. Both phases were dose-dependent but the potency of elevation at peak was significantly less than that of the sustained phase. Bradykinin also dose-dependently stimulated Ins(1,4,5)P3 accumulation but responses were smaller and not sustained. the ability of agonists for predominantly sustained PLC activation, potentially driven by a partially desensitized receptor, as opposed to rapid activation by a fully sensitized receptor. 8 These data provide quantitative comparisons between and within components of the receptormediated phosphoinositide and Ca24 signalling pathway, provide mechanistic insights into regulation of these components and characterize a model system in which heterologous interaction between two receptors linked to the same transduction pathway may be examined.

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Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Willars

Nahorski

1995

British J Pharmacology

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Bradykinin-evoked Release of [3H]Noradrenaline from the Human Neuroblastoma SH-SY5Y

Abstract: Our previous studies have shown that cultures of the human neuroblastoma SHSYSY express depolarisation-evoked release of NA stimulated by elevated potassium [ 1.21 or activation of nicotinic receptors [3] which is coupled to L-type calcium channels. In

Cited by 1 publication

References 6 publications

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

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Bradykinin-evoked Release of [3H]Noradrenaline from the Human Neuroblastoma SH-SY5Y

Abstract: Our previous studies have shown that cultures of the human neuroblastoma SHSYSY express depolarisation-evoked release of NA stimulated by elevated potassium [ 1.21 or activation of nicotinic receptors [3] which is coupled to L-type calcium channels. In

Cited by 1 publication

References 6 publications

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells

Contact Info

Product

Resources

About

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells