Bradykinin promotes migration and invasion of human immortalized trophoblasts

Erices, Rafaela; Corthorn, Jenny; Lisboa, Francisco; Valdés, Gloría

doi:10.1186/1477-7827-9-97

Cited by 29 publications

(27 citation statements)

References 64 publications

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“…However, the fluorescent immunocytochemistry expression pattern in the cytoplasm differed in canine trophoblasts such that MMP2 was concentrated in large coalescing granules whereas MMP9 was more diffusely expressed throughout the cell. A similar staining pattern was reported in human trophoblasts for MMP2 and MMP9 (Erices et al 2011).…”

Section: Discussionsupporting

confidence: 85%

Cellular Characteristics of Cultured Canine Trophoblasts

Sahlfeld

Hazzard

Kutzler

2012

Reprod Domestic Animals

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Contents Shallow trophoblast invasion is detrimental in human pregnancies, but represents normal endotheliochorial placentation in dogs. Factors regulating shallow trophoblast invasion into the canine decidua are not well described, but it is known that matrix metalloproteinases (MMPs) play a crucial role in trophoblast invasion in many species. Following the methods previously described for isolating human trophoblasts, canine trophoblasts were isolated using collagenase and trypsin digestions with Percoll density gradient centrifugation. In addition, placental pieces were cryopreserved prior to primary culture following methods previously described for human tissue. Expression of cytokeratin‐7, MMP2 and MMP9 was confirmed using fluorescent immunocytochemistry. Cellular morphology was similar to that reported for trophoblasts. More than 97% of the cells cultured expressed cytokeratin‐7. More cultured canine trophoblasts expressed MMP9 (54.7 ± 3.4%) compared with MMP2 (40.3 ± 1.8%) (p = 0.02). Although both MMPs were immunolocalized to the cytoplasm, MMP2 was found in large, coalescing granules, whereas MMP9 was more diffusely expressed throughout the cell. Cryogenic freezing of placental tissue prior to primary cell culture had no effect on cell proliferation (p = 0.37). This research has established a baseline for future studies investigating the canine placenta as a model for disorders of shallow trophoblast invasion in humans.

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Section: Discussionsupporting

confidence: 85%

Cellular Characteristics of Cultured Canine Trophoblasts

Sahlfeld

Hazzard

Kutzler

2012

Reprod Domestic Animals

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“…The B2R-induced migration and invasion in immortalized extravillous trophoblasts recently described by the authors of this paper56 supports the role of the KKS in placentation.…”

Section: Vasodilator Factors and Their Systemic Response In Normotenssupporting

confidence: 87%

Challenges posed to the maternal circulation by pregnancy

Valdés

Corthorn²

2011

IBPC

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In primates, adequate growth of the fetus depends on the development of the uteroplacental unit. On the fetal side, this is achieved by the creation of the vascular network of the placenta. On the maternal side, the transformation of the spiral arteries into saccular nonreactive vessels by the trophoblast provides high blood flow to the intervillous space. Apart from the changes in the uterine arteries, the mother expands her plasma volume – at the expense of stimulating the renin-angiotensin-aldosterone system – and her cardiac output. In the maintaining of normotension in the face of an increased cardiac output and plasma volume, the renin-angiotensin-aldosterone system requires an enhanced vasodilator synthesis. Finally, in the late stages of pregnancy, a normal endothelial function is required to provide an ample margin to the activation provoked by deportation of syncytiotrophoblast fragments/factors to the maternal circulation. These four adaptative processes require various interrelated vasodilator systems. Deficient adaptations cause isolated or proteinuric arterial hypertension, intrauterine growth restriction, preterm delivery, and stillbirths, among others. Moreover, a normal or a defective adaptation to pregnancy influences maternal cardiovascular health in later life, as evidenced by various studies, most of them epidemiological; thus, pregnancy is now considered a stress test to the maternal cardiovascular system. Because of this, women planning to become pregnant should be screened for clinical and biochemical cardiovascular risks. Inversely, women presenting with hypertension in pregnancy should be thoroughly studied to detect and correct cardiovascular risks. The incorporation of the predictive value of a hypertensive pregnancy should help reduce cardiovascular disease in women.

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“…Consistent with a guidance function for xBdk, midline or lateral placement (into the EAD) of xBdk-impregnated beads was sufficient to overcome the NC migration defect after Kinin-Kallikrein LOF. Bradykinin is pro-migratory in other settings, for malignant cells and trophoblasts, while NO is involved in inflammation-induced cell migration (Chen et al 2000; Cuddapah et al 2013; Erices et al 2011; Yu et al 2013). Interestingly, another substrate for CPN is C3a, a small complement peptide required for more local aspects of cranial NC migration (Carmona-Fontaine et al, 2011; Matthews K., 2004).…”

Section: Discussionmentioning

confidence: 99%

The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling

et al. 2014

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SUMMARY The extreme anterior domain (EAD) is a conserved embryonic region that includes the presumptive mouth. We show that the Kinin-Kallikrein pathway is active in the EAD and necessary for craniofacial development in Xenopus and zebrafish. The mouth failed to form and neural crest (NC) development and migration was abnormal after loss of function (LOF) in the pathway genes kng, encoding Bradykinin (xBdk), carboxypeptidase-N (cpn) that cleaves Bradykinin and neuronal nitric oxide synthase. Consistent with a role for nitric oxide (NO) in face formation, endogenous NO levels declined after LOF in pathway genes but these were restored and a normal face formed after medial implantation of xBdk-beads into LOF embryos. Facial transplants demonstrated that Cpn function from within the EAD is necessary for migration of first arch cranial NC into the face and to promote mouth opening. The study identifies the EAD as an essential craniofacial organizer acting through Kinin-Kallikrein signaling.

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Bradykinin promotes migration and invasion of human immortalized trophoblasts

Cited by 29 publications

References 64 publications

Cellular Characteristics of Cultured Canine Trophoblasts

Cellular Characteristics of Cultured Canine Trophoblasts

Challenges posed to the maternal circulation by pregnancy

The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling

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