Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil

Michel, Daniele Cabral; Passos, Samuel Ribeiro; Simões‐Araújo, Jean Luiz; Baraúna, Alexandre Cardoso; Silva, Krisle da; Parma, M. M.; Melo, I. S. de; Meyer, Sofie E. De; O’Hara, G.W.; Zilli, Jerri Édson

doi:10.1007/s00203-017-1340-y

“…Yet, as recently noted by Grönemeyer and Reinhold-Hurek [ 23 ], our knowledge on the diversity and biogeography of bradyrhizobia species relies primarily on data collected in the Americas, Asia and Europe. For examples, type strains of the B. elkanii supergroup include B. icense LMTR 13 T isolated from Phaseolus lunatus in Peru [ 24 ]; B. macuxiense UFLA03-321 T found inside a nodule of Centrolobium parense in Amazonia [ 25 ] and B. retamae Ro19 T identified as a symbiont of Retama monosperma in the Mediterranean basin [ 26 ]. A number of recent surveys identified bradyrhizobia in sub-Saharan countries, however, including in Angola and Namibia [ 27 ], Ethiopia [ 14 ], Kenya [ 28 ] and Senegal [ 29 ].…”

mentioning

confidence: 99%

Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire

Fossou

¹

,

Pothier

²

,

Zeze

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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For many smallholder farmers of Sub-Saharan Africa, pigeonpea ( Cajanus cajan ) is an important crop to make ends meet. To ascertain the taxonomic status of pigeonpea isolates of Côte d’Ivoire previously identified as bradyrhizobia, a polyphasic approach was applied to strains CI-1B T , CI-14A, CI-19D and CI-41S. Phylogeny of 16S ribosomal RNA (rRNA) genes placed these nodule isolates in a separate lineage from current species of the B. elkanii super clade. In phylogenetic analyses of single and concatenated partial dnaK , glnII , gyrB , recA and rpoB sequences, the C. cajan isolates again formed a separate lineage, with strain CI-1B T sharing the highest sequence similarity (95.2 %) with B. tropiciagri SEMIA 6148 T . Comparative genomic analyses corroborated the novel species status, with 86 % ANIb and 89 % ANIm as the highest average nucleotide identity (ANI) values with B. elkanii USDA 76 T . Although CI-1B T , CI-14A, CI-19D and CI-41S shared similar phenotypic and metabolic properties, growth of CI-41S was slower in/on various media. Symbiotic efficacy varied significantly between isolates, with CI-1B T and CI-41S scoring on the C. cajan ‘Light-Brown’ landrace as the most and least proficient bacteria, respectively. Also proficient on Vigna radiata (mung bean), Vigna unguiculata (cowpea, niébé) and additional C. cajan cultivars, CI-1B T represents a potential bioinoculant adapted to local soil conditions and capable of fostering the growth of diverse legume crops in Côte d'Ivoire. Given the data presented here, we propose the 19 C . cajan isolates to belong to a novel species called Bradyrhizobium ivorense sp. nov., with CI-1B T (=CCOS 1862 T =CCMM B1296 T ) as a type strain.

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“…y Bradyrhizobium spp. estas pertenecen al grupo de bacterias gram-negativas quimioorganótrofas (Michel et al, 2017), son los géneros de bacterias fijadoras de nitrógeno que comprenden la mayoría de la población microbiana de los nódulos de leguminosas, tanto α-Rhizobia (miembros de la Alphaproteobateria, por ejemplo, Rhizobium y Bradyrhizobium) como β-Rhizobia (Betaproteobacteria, por ejemplo, Cupriavidus y Burkholderia), son las más conocidas y estudiadas formadoras de nódulos en leguminosas (Gyaneshwar et al, 2011).…”

Section: Análisis Cuantitativounclassified

FACTORES QUE AFECTAN LA FIJACIÓN DE NITRÓGENO EN EL SISTEMA Inga spp. -RIZOBIOS EN CAFETALES DE SOMBRA

Velasco-Trejo

¹

,

Alarcón-Gutiérrez

²

,

García-Pérez

³

et al. 2023

Trop Subtrop Agroecosyst

1

0

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Background. The results of a review on the factors that affect nitrogen fixation in the Inga-café system are presented. The nitrogen contribution that the Inga Miller tree genus provides to shade coffee plantations and the factors that affect its stability in biological nitrogen fixation are scarcely recognized. This work delves into the ecological factors and glyphosate herbicides that can affect the symbiosis of Inga spp. with diazotrophic bacteria in their roots (rhizobia), which form nodules and carry out biological nitrogen fixation. Methodology. Different information sources such as EBSCO, Scopus and Google Schoolar were reviewed, with logical or Boolean data search. 183 papers were used to address the factors that affect the symbiosis between trees of the Inga genus and rhizobia. The findings were organized in: Inga Miller taxonomy, shade coffee plantations with Inga spp., symbiosis between Inga spp. and Bradyrhizobium spp., ecological factors that affect the functioning of the Inga spp.-rhizobia symbiosis and the agrochemical factor: glyphosate herbicide. Results. The genus Inga is recognized as a clade Mimosoideae nested in the subfamily Caesalpinioideae. Shade coffee plantations with Inga are established below 23º N and 30º S, up to 3,100 m.a.s.l. and the symbiont genus is predominantly Bradyrhizobium spp. whose ecological limits of Inga spp-rhizobia were: altitude [988.7-1,381.5 m.a.s.l.], annual precipitation [2,048.4-2,064.36 mm], temperature [20.39-21.93 ºC] and soil pH [4.88-5.42 pH]. Glyphosated herbicides, as an external stimulus, can reduce the benefit of biological nitrogen fixation and erode the soil by keeping it devoid of vegetation. Additionally, some Bradyrhizobium spp. (thiO gene), which oxidizes glyphosate to aminomethylphosphonic acid (AMPA), could be a potential glyphosate degrader in the soil. Implications. Shade coffee plantations with 205-250 trees per hectare of Inga spp. allow fixing around 45 kg of N ha-1 year-1. This review may allow the adoption of new observational or experimental studies of the Inga spp.-rhizobia symbiosis, to approach the performance that favors the biological fixation of nitrogen in shade coffee plantations. Conclusions. The review indicates that there is a specific association between Inga spp. and Bradyrhizobium spp., that ecological factors, including the agronomic management with glyphosated herbicides can decrease nitrogen fixation performance during the symbiosis between Inga spp. and rhizobia. No systematic studies of the symbiosis-environment-agrochemical interaction in shade coffee plantations were found.

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“…The enzymatic activities were analysed by the use of the API 20NE kit (bioMérieux), while carbon sources assimilation was tested with the GNIII MicroPlate system (Biolog), following the method previously described [41].…”

Section: Phenotypementioning

confidence: 99%

Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.

Ferreira

¹

,

Coniglio

²

,

Puente

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Strain Az39T of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3-acetic acid in the presence of l-tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the cells are curved or spiral-shaped, with diameters ranging from 0.5–0.9 to 1.8–2.2 µm. They contain C16 : 0, C18 : 0 and C18 : 1 ω7c/ω6c as the main fatty acids. Phylogenetic analysis of its 16S rRNA gene sequence confirmed that this strain belongs to the genus Azospirillum , showing a close relationship with Azospirillum baldaniorum Sp245T, Azospirillum brasilense Sp7T and Azospirillum formosense CC-Nfb-7T. Housekeeping gene analysis revealed that Az39T, together with five strains of the genus (Az19, REC3, BR 11975, MTCC4035 and MTCC4036), form a cluster apart from A. baldaniorum Sp245T, A. brasilense Sp7T and A. formosense CC-Nfb-7T. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Az39T and the aforementioned type strains revealed values below 96 %, the circumscription limit for the species delineation (ANI: 95.3, 94.1 and 94.0 %; dDDH: 62.9, 56.3 and 55.6 %). Furthermore, a phylogeny evaluation of the core proteome, including 809 common shared proteins, showed an independent grouping of Az39T, Az19, REC3, BR 11975, MTCC4035 and MTCC4036. The G+C content in the genomic DNA of these six strains varied from 68.3 to 68.5 %. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we consider that strain Az39T, along with strains Az19, REC3, BR 11975, MTCC4035 and MTCC4036, are members of a new Azospirillum species, for which the name Azospirillum argentinense sp. nov. is proposed. The type strain is Az39T (=LBPCV39T=BR 148428T=CCCT 22.01T).

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Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil

Cited by 37 publications

References 21 publications

Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire

Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire

FACTORES QUE AFECTAN LA FIJACIÓN DE NITRÓGENO EN EL SISTEMA Inga spp. -RIZOBIOS EN CAFETALES DE SOMBRA

Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.

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