Brahmarasayana protects against Ethyl methanesulfonate or Methyl methanesulfonate induced chromosomal aberrations in mouse bone marrow cells

Guruprasad, Kanive Parashiva; Subramanian, Advait; Singh, Vikram Jeet; Sharma, Raghavendra Sudheer Kumar; Gopinath, P. M.; Sewram, Vikash; Varier, Panniyampilly Madhavankutty; Satyamoorthy, Kapaettu

doi:10.1186/1472-6882-12-113

Cited by 14 publications

(14 citation statements)

References 32 publications

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“…However, with EMS at 72 h RT and MMS at 48 and 72 h in both diabetic and non diabetic mice with different doses, the activity of catalase did not exhibit significant changes, thus, it can be opined that the changes in the activity of catalase were not dose dependent. Working in non diabetic mice Guruprasad et al [44] have demonstrated an increase in catalase activity with EMS and decreased activity with MMS treatment which is on par with our studies in non diabetic mice. Salmon et al [45] and Friedberg et al [46] have showed that MMS cannot produce ROS directly, but induced the DNA damage causing an increase in intracellular ROS level in yeast cells.…”

Section: Discussionsupporting

confidence: 86%

Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

Khalandar

Vasudev

2015

Egyptian Journal of Medical Human Genetics

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Aim: To study the effect of alkylating agents such as EMS and MMS on chromosomes and biochemical parameters in induced diabetic mouse.Methods: Chromosome preparations from bone marrow was made using the method of Evans et al. (1964) and biochemical estimations from the liver was done by the method of Sinha (1972) for catalase, Van der Vies (1954) for glycogen and Uchiyama and Mihara (1978) for MDA.Results: The study has revealed that EMS and MMS induced a dose dependent increase in chromosomal aberrations of chromatid type in the diabetic mouse. Nonetheless, it is interesting to note that, there is significant reduction in the frequency of chromosomal aberrations in diabetic compared to non diabetic mice at all tested doses of EMS or MMS and at different recovery times [RTs]. On the other hand biochemical parameters showed a variable degree of reactivity: (1) catalase activity was significantly elevated in non diabetics whereas in diabetics it is significantly decreased with increasing concentrations of EMS. Contrary to this, the catalase activity in the case of MMS treatment is significantly reduced in non diabetics compared to diabetic mice. (2) However glycogen level is significantly reduced in both the diabetic and non diabetic with increasing concentrations of EMS or MMS, but MDA levels were significantly increased.Conclusion: (1) Even though alkylating agents induce chromosomal aberrations in diabetic mice, MMS, a methylating agent is a more potent inducer of chromatid type of aberrations than EMS, an ethylating agent. (2) Diabetic mouse is more resistant than the non diabetic to alkylating agents and (3) the tested agents altered the analyzed biochemical parameters. Ó 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of Ain Shams University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Please cite this article in press as: Khalandar BBD, Vasudev V, Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse, Egypt J Med Hum Genet (2015), http://dx.

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Section: Discussionsupporting

confidence: 86%

Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

Khalandar

Vasudev

2015

Egyptian Journal of Medical Human Genetics

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“…In general, the toxic mechanism of action involves the direct alkylation by type 2 nucleophilic substitution of guanine and adenine, which results in their ability to induce single and double strand breaks, as well as to generate reactive oxygen species [33]. As a whole, the data suggest that the protective effect of the prickly pear juice red-purple variety is associated with the presence of phytochemicals of their own chemical composition, and although our data do not confirm it completely, we think that the anticlastogenic ability is probably attributed to a synergistic antioxidant mechanism between betalains and flavonoids as they are the main components.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice

et al. 2013

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Plants belonging to the genus Opuntia spp. are the most abundant of the Cactaceae family, grown throughout America and the Mediterranean central area. Its fruit, known as cactus pear or prickly pear, is an oval berry grouped in different colors. Some studies have shown its antioxidant activities which may help in preventing chronic pathologies such as diabetes. The purpose of the study was to evaluate the antioxidant capacity of three varieties of prickly pear juice (red-purple, white-green and yellow-orange) in five different concentrations (100, 250, 500, 750, and 1000 mg/mL) by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) colorimetric method, selecting the best variety to determine its anticlastogenic potential against methyl methanesulfonate (MMS). The results indicate that the highest antioxidant was found in the juice of the prickly pear red-purple variety (PPRP), in all concentrations. Its anticlastogenic potential was therefore evaluated with a micronucleus assay. The experiment was run over two weeks. A negative control was included along with a positive control with MMS (40 mg/kg), a group of mice treated with PPRP (25 mL/kg), and three groups with PPRP (in doses of 25, 16.5 and 8.3 mL/kg) plus the mutagen. The PPRP was administered daily by oral gavage and the MMS was injected intraperitoneally five days prior to the end of the experiment. Blood samples were obtained at 0, 24, 48, 72 and 96 h in order to determine the frequency of micronucleated polychromatic erythrocytes (MNPE). The results indicated that PPRP is not a genotoxic agent, on the contrary, it may reduce the number of MNPE. In this regard, the PPRP showed an anticlastogenic effect directly proportional to its concentrations. Thus, the highest protection was obtained with a concentration of 25 mL/kg after 48 h of treatment.

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“…EMS was often used as a positive control in genotoxic test, both in in vitro and in vivo test system. The types of chromosomal aberrations induced by EMS as a positive control were reported to be chromosome break, chromatid break (14). According to our knowledge, this is the fi rst study that addresses the antigenotoxic effects of 4-MEI in bone marrow cells of Swiss Albino Mice.…”

Section: Discussionmentioning

confidence: 99%

“…Ethyl methanesulfonate (EMS) well-known mutagenic and clastogenic agents in in vivo test system were used as a genotoxic agent (14). Sub-lethal high doses of EMS (240 mg/kg b.w) was employed (12 or 24 hours before sacrifi ced of the animals), as earlier established by Riaz and Vasudev (15).…”

Section: Ems Administrationmentioning

confidence: 99%

Delineating the antigenotoxic and anticytotoxic potentials of 4-methylimidazole against ethyl methanesulfonate toxicity in bone marrow cell of swiss albino mice

Tazehkand¹,

Topaktaş²,

Yilmaz³

et al. 2016

BLL

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4-Methylimidazole (4-MEI) is mostly used in beverages and coloring food, dark beers and common brands of cola drinks, which may contain more than 100 μg of this compound per 12-ounce serving. This study was aimed to investigate the antigenotoxic and anticytotoxic effects of 4-MEI (100, 130 and 160 mg/kg) against ethyl methanesulfonate (240 mg/kg) using chromosome aberrations (CAs) and Mitotic index (MI) tests in bone marrow cells of Swiss Albino Mice at 12 h and 24 h treatment periods. So, the t-test was used for the statistical analysis. In this research, 4-MEI at all concentrations for 12 h treatment period reduced chromosomal aberrations and at 130 and 160 mg/kg concentrations for 24 h treatment period increased chromosomal aberrations induced by EMS (240 mg/kg), but th ese reductions and increases were not signifi cant. Also, intraperitoneal injection of 4-MEI at doses of 100, 130 and 160 mg/kg combined with EMS (240 mg/kg) showed that the mitotic index was decreased at 100 and 130 mg/kg for 12h and 130 mg/kg for 24 h treatment periods, when compared to positive sample (EMS), but did not show any statistically difference from the EMS treated group. It can be concluded that 4-MEI might not be antigenotoxic and protective effects in bone marrow cells of Swiss Albino Mice, because 4-MEI could not reduce the chromosomal aberrations induced by EMS (Tab. 2, Fig. 2, Ref. 36). Text in PDF www.elis.sk.

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Brahmarasayana protects against Ethyl methanesulfonate or Methyl methanesulfonate induced chromosomal aberrations in mouse bone marrow cells

Cited by 14 publications

References 32 publications

Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice

Delineating the antigenotoxic and anticytotoxic potentials of 4-methylimidazole against ethyl methanesulfonate toxicity in bone marrow cell of swiss albino mice

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