Brain and pituitary Fibroblast Growth Factor activities behave identically on three independent high performance liquid chromatography systems

Smith, John A.; Winslow, Derek P.; O’Hare, Michael J.; Rudland, Philip S.

doi:10.1016/0006-291x(84)91653-x

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…One factor, pituitary-derived mammatrophic growth factor (PMGF), although only partially purified, stimulates the growth of only the cuboidal epithelial cells and cell lines from normal rat mammary glands and their benign tumors [82,179]. The second growth factor, fibroblast growth factor (FGF) [181,182], which has now been purified to homogeneity [183], stimulates growth of only the elongated, myoepithelial-like and stromal cell lines [179]. The activity of PMGF in rat pituitary glands and blood, as measured by the increase in growth of Rama 25 epithelial cells in vitro is approximately correlated with the growth state of the mammary gland in vivo [179].…”

Section: (10) Identification Of Growth Factors For Cultured Mammary Cmentioning

confidence: 99%

Stem cells and the development of mammary cancers in experimental rats and in humans

Rudland

1987

Cancer Metast Rev

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Evidence based on immunocytochemical staining and ultrastructure suggests that morphological gradations between epithelial and myoepithelial cells, and possibly between epithelial cells and alveolar-like cells occur in terminal ductal structures of rat and human mammary glands. The benign carcinogen-induced rat and benign human mammary tumors can contain epithelial, myoepithelial-like and alveolar-like cells, whereas the malignant counterparts mainly contain only epithelial-like cells. Clonal epithelial cell lines from normal rat mammary glands, benign tumors, and SV40-transformed human mammary glands can differentiate to either myoepithelial-like or alveolar-like cells. In those of the rat, the differentiation processes occur in steps: intermediate cells along the myoepithelial-like pathway resemble intermediates in terminal ductal structures in vivo, and can also generate certain well-differentiated mesenchymal elements of the original tumours. Differentiation of the benign rat cells to alveolar-like cells with mammatrophic hormones and retinoids in vitro leads to a reduction in their tumor-forming ability in vivo. Cell lines from malignant rat mammary tumors of increasing metastatic potential and from human ductal carcinomas largely fail to yield myoepithelial-like or alveolar-like cells and are relatively slow-growing. Growth of the rat mammary epithelial cells in culture is stimulated by a pituitary-derived mammatrophic growth factor (PMGF), prostaglandin E2, and alpha-transforming growth factor; the response of the malignant cell lines to PMGF is reduced. It is suggested that stem cells exist in the rat and human glands that are capable of differentiating to the other major cell types of the mammary parenchyma, and that during the carcinogenic process they generate genetically unstable cells which lose their ability to differentiate and attempt to maximise their intrinsically slow growth rate.

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Section: (10) Identification Of Growth Factors For Cultured Mammary Cmentioning

confidence: 99%

Stem cells and the development of mammary cancers in experimental rats and in humans

Rudland

1987

Cancer Metast Rev

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“…Individual FGFs play important roles in various physiological and pathological processes, including embryonic development, cell growth, morphogenesis, tissue repair, inflammation, angiogenesis, and tumor growth and invasion (30). The first characterized members of the FGF family were acidic FGF (aFGF or FGF-1) and basic FGF (bFGF or FGF-2), which were purified as mitogens for fibroblasts from the pituitary and brain (7,9,12,23,41). Subsequently, it became apparent that these growth factors were able to promote the growth of mesodermal and neuroectodermal cells during both embryogenesis and adulthood (14,15).…”

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confidence: 99%

FGF-18, a Novel Member of the Fibroblast Growth Factor Family, Stimulates Hepatic and Intestinal Proliferation

Qiu

Wang

et al. 1998

Molecular and Cellular Biology

134

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The fibroblast growth factors (FGFs) play key roles in controlling tissue growth, morphogenesis, and repair in animals. We have cloned a novel member of the FGF family, designated FGF-18, that is expressed primarily in the lungs and kidneys and at lower levels in the heart, testes, spleen, skeletal muscle, and brain. Sequence comparison indicates that FGF-18 is highly conserved between humans and mice and is most homologous to FGF-8 among the FGF family members. FGF-18 has a typical signal sequence and was glycosylated and secreted when it was transfected into 293-EBNA cells. Recombinant murine FGF-18 protein (rMuFGF-18) stimulated proliferation in the fibroblast cell line NIH 3T3 in vitro in a heparan sulfate-dependent manner. To examine its biological activity in vivo, rMuFGF-18 was injected into normal mice and ectopically overexpressed in transgenic mice by using a liver-specific promoter. Injection of rMuFGF-18 induced proliferation in a wide variety of tissues, including tissues of both epithelial and mesenchymal origin. The two tissues which appeared to be the primary targets of FGF-18 were the liver and small intestine, both of which exhibited histologic evidence of proliferation and showed significant gains in organ weight following 7 (sometimes 3) days of FGF-18 treatment. Transgenic mice that overexpressed FGF-18 in the liver also exhibited an increase in liver weight and hepatocellular proliferation. These results suggest that FGF-18 is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine.

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Relationship of growth factors and differentiation in normal and neoplastic development of the mammary gland

Fernig¹,

Smith²,

Rudland³

1991

Regulatory Mechanisms in Breast Cancer

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Brain and pituitary Fibroblast Growth Factor activities behave identically on three independent high performance liquid chromatography systems

Cited by 8 publications

References 14 publications

Stem cells and the development of mammary cancers in experimental rats and in humans

Stem cells and the development of mammary cancers in experimental rats and in humans

FGF-18, a Novel Member of the Fibroblast Growth Factor Family, Stimulates Hepatic and Intestinal Proliferation

Relationship of growth factors and differentiation in normal and neoplastic development of the mammary gland

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