Brain Death Caused by Electric Shock and Organ Donation in Children

Todeschini, D. P.; Maito, E.D.M.; Maldotti, A.; Moreira, Alejandra; Capaverde, F.B.; Figueiredo, F.M.; Oliveira, D.M.S.; Costa, Maria Gabriella; Hoelfmann, N.; Londero, G.G.; Garcı́a, Valter Duro

doi:10.1016/j.transproceed.2007.01.033

Cited by 4 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, SH_elec showed superior functionality and viability to SH_gas during the 5 hours of perfusion. With respect to transplantation, electrocution is not a contraindication for organ donation, 29 and in the present study, electrical stunning had less damaging effects on the liver than CO 2 stunning. Terlouw et al showed that there was less severe acidosis in the muscles of electrically stunned animals than in CO 2 -stunned animals, and the less acidic environment of livers from electrically stunned animals may explain the physiological values obtained for SH_elec.…”

Section: Discussionsupporting

confidence: 59%

See 1 more Smart Citation

Are slaughterhouse-obtained livers suitable for use in ex vivo perfusion research?

Ruppelt,

Pijnenburg,

Pappers

et al. 2023

J Int Med Res

View full text Add to dashboard Cite

Objectives The success of the ex vivo machine perfusion of pig livers used for preclinical research depends on organ quality and availability. In this study, we investigated whether livers obtained from slaughterhouses are suitable and equivalent to livers obtained from laboratory pigs. Methods Livers were obtained from slaughterhouse pigs stunned by electrocution or CO2 inhalation and from laboratory pigs. For the latter group, 45 minutes of warm ischemia was mimicked for a subgroup, ensuring a valid comparison with slaughterhouse-derived livers. Results Livers from CO2-stunned pigs showed lower indocyanine green clearance and bile production, higher blood lactate and potassium concentrations, and higher alanine aminotransferase activities than electrically stunned pigs. Furthermore, livers from electrically stunned pigs, and livers from laboratory pigs, subjected or not to warm ischemia, showed similar performance in terms of perfusion and metabolism. Conclusion For an ex vivo liver model generated using slaughterhouse pigs, electrical stunning is preferable to CO2 stunning. Livers from electrically stunned slaughterhouse pigs performed similarly to laboratory pig livers. These findings support the use of livers from electrically stunned slaughterhouse pigs, which may therefore provide an alternative to livers obtained from laboratory pigs, consistent with the principle of the 3Rs.

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 59%