Brain-derived neurotrophic factor in human platelets

Pliego-Rivero, F. Bernardo; Bayatti, Nadhim; Giannakoulopoulos, Xenophon; Glover, Vivette; Bradford, H. F.; Stern, Gerald; Sandier, M.

doi:10.1016/s0006-2952(97)00073-7

Cited by 47 publications

(25 citation statements)

References 14 publications

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“…TNF-α and HGF, by disrupting NFkB-transactivating activity, are important for the anti-inflammatory function in this context. BDNF is known to be an important member of the neurotrophic family with neural growth and differentiation properties and the large amounts of circulating BDNF proteins are stored in platelets [60,61]. It reaches the maximum accumulation at 1day (about 30000 pg) and after this maintains a plateau.…”

Section: Discussionmentioning

confidence: 99%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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SummaryScientific background: Platelet concentrates are nowadays widely applied in different clinical fields to improve soft tissue and bone regeneration. "Concentrated Growth Factors" (CGF) is a new generation of platelet concentrate products, which exhibits an interesting clinical and biotechnological application potential. Aim of the study:The aim of this study is to assess the biological rationale for the use of CGF, by evaluating blood cell localization, the in vitro cumulative release of seven growth factors (PDGF-AB, VEGF, TNF-α, TGF-β1, BDNF, BMP-2 and IGF-1), its in vitro effects on cell proliferation and its mechanical behavior.Methods: CGFs were obtained from volunteer donors. Blood cell localization was evaluated after properly morphological staining and immunohistochemistry. The amount of growth factors release was measured at 5 hours, 1, 3, 6, 7 and 8 days, using ELISA assay. Cells were cultured with and without CGF and their proliferation was evaluated after 72 hours, performing the quantification of Ki-67, using flow cytometry (FACS). The mechanical response of CGF under compression was also attempted. Results:The results showed that platelets and leukocytes were found in a very thin space called "buffy coat", localized between the white and red part of CGF. Each growth factor evaluated, had a specific kinetic release with a great variability among subjects. The in vitro cell proliferation was stimulated. CGF showed an "apparent plasticity" and its mechanical response was influenced by fibrin network structure. Conclusion:These findings support the CGF's clinical use and will allow us to better understand and improve the clinical outcomes. B io lo g y and M e d ic in e

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Section: Discussionmentioning

confidence: 99%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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“…In order to obtain measures of pBDNF from serum, samples of whole blood must be allowed to clot prior to collecting aliquots for the performance of ELISAs. Blood platelets have been shown to contain high concentrations of BDNF, 26 and the radical difference between concentrations of BDNF in serum compared to plasma have been suggested to be due to the degradation of platelets during clotting which subsequently releases BDNF into the serum. 25,27 In their review, Knaepen et al 9 noted these differences in concentrations of pBDNF when assessed in samples from serum compared to plasma and suggested that assessments from plasma reflected concentrations of free-circulating BDNF, while assessments from serum reflected concentrations of BDNF stored in platelets and immune cells.…”

Section: Source Of Bdnf Measuresmentioning

confidence: 99%

Brain-derived neurotrophic factor (BDNF) as a potential mechanism of the effects of acute exercise on cognitive performance

Piepmeier

Etnier

2015

Journal of Sport and Health Science

168

112

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The literature shows that improvements in cognitive performance may be observed following an acute bout of exercise. However, evidence in support of the biological mechanisms of this effect is still limited. Findings from both rodent and human studies suggest brain-derived neurotrophic factor (BDNF) as a potential mechanism of the effect of acute exercise on memory. The molecular properties of BDNF allow this protein to be assessed in the periphery (pBDNF) (i.e., blood serum, blood plasma), making measurements of acute exercise-induced changes in BDNF concentration relatively accessible. Studies exploring the acute exerciseepBDNFecognitive performance relationship have had mixed findings, but this may be more reflective of methodological differences between studies than it is a statement about the role of BDNF. For example, significant associations have been observed between acute exercise-induced changes in pBDNF concentration and cognitive performance in studies assessing memory, and non-significant associations have been found in studies assessing non-memory cognitive domains. Three suggestions are made for future research aimed at understanding the role of BDNF as a biological mechanism of this relationship: 1) Assessments of cognitive performance may benefit from a focus on various types of memory (e.g., relational, spatial, long-term); 2) More finegrained measurements of pBDNF will allow for the assessment of concentrations of specific isoforms of the BDNF protein (i.e., immature, mature); 3) Statistical techniques designed to test the mediating role of pBDNF in the acute exercise-cognitive performance relationship should be utilized in order to make causal inferences.

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“…29 Thus, the difference between serum and plasma levels of BDNF could reflect the amount of BDNF stored in platelets. 29 Circulating platelets may not only serve as a transportation system for BDNF, but also as a repository that can release large amounts of BDNF in response to the acute demands of specific organs. 26 Brain-derived neurotrophic factor is also involved in the cognitive dysfunction that occurs in patients with OSAS.…”

Section: Regulation Of Bdnf In Obstructive Sleep Apnoeamentioning

confidence: 99%

Changes of Serum Brain-Derived Neurotrophic Factor in Children with Obstructive Sleep Apnoea–Hypopnoea Syndrome following Adenotonsillectomy

Wang

Zhao

et al. 2010

J Int Med Res

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Children with obstructive sleep apnoea syndrome (OSAS) have substantial cognitive functional morbidity. Brainderived neurotrophic factor (BDNF) is a key mediator of memory and cognition, but its regulation in OSAS is unknown. Circulating BDNF, transforming growth factor-b 1 and 5-hydroxytryptamine levels, cognitive ability and overnight polysomnography were evaluated in 44 children with newly-diagnosed OSAS and in 26 healthy children. All parameters were monitored pre-operatively and at 3, 6 and 12 months after adenotonsillectomy. Pre-operative cognitive ability and sleep parameters were significantly poorer in children with OSAS compared with controls, but BDNF levels were similar. At 3 months post-operation, serum BDNF concentrations decreased, but cognitive ability and sleep parameters remained deficient. At 6 months post-operation, serum BDNF levels, sleep parameters and cognitive ability had improved and, by 12 months, there were no differences between the two groups. It is concluded that adenotonsillectomy can rapidly normalize sleep parameters and improve the cognitive ability of children with OSAS, by regulating the circulating level of BDNF.

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Brain-derived neurotrophic factor in human platelets

Cited by 47 publications

References 14 publications

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Brain-derived neurotrophic factor (BDNF) as a potential mechanism of the effects of acute exercise on cognitive performance

Changes of Serum Brain-Derived Neurotrophic Factor in Children with Obstructive Sleep Apnoea–Hypopnoea Syndrome following Adenotonsillectomy

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