Brain DNA damage and behavioral changes after repeated intermittent acute ethanol withdrawal by young rats

Costa, Priscila A.; Poli, Jefferson H. Z.; Sperotto, Nathalia Denise de Moura; Moura, Dinara Jaqueline; Saffi, Jenifer; Nin, Maurício Schüler; Barros, Helena Maria Tannhauser

doi:10.1007/s00213-015-4015-x

Cited by 11 publications

(3 citation statements)

References 64 publications

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“…; Costa et al . ), an effect that may be both a direct effect of ethanol on the generation of ROS or a secondary effect of increases in TNF‐α that are reported to activate mitochondrial reactive oxygen generation (Kastl et al . ), which could lead to a vicious cycle where neuroinflammation and ROS potentiate and perpetuate each other.…”

Section: Discussionmentioning

confidence: 99%

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Activated mesenchymal stem cell administration inhibits chronic alcohol drinking and suppresses relapse‐like drinking in high‐alcohol drinker rats

Ezquer

Quintanilla²,

Morales³

et al. 2017

Addiction Biology

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Neuroinflammation has been reported to follow chronic ethanol intake and may perpetuate alcohol consumption. Present studies determined the effect of human mesenchymal stem cells (hMSCs), known for their anti-inflammatory action, on chronic ethanol intake and relapse-like ethanol intake in a post-deprivation condition. Rats were allowed 12-17 weeks of chronic voluntary ethanol (10% and 20% v/v) intake, after which a single dose of activated hMSCs (5 × 10 ) was injected into a brain lateral ventricle. Control animals were administered vehicle. After assessing the effect of hMSCs on chronic ethanol intake for 1 week, animals were deprived of ethanol for 2 weeks and thereafter an ethanol re-access of 60 min was allowed to determine relapse-like intake. A single administration of activated hMSCs inhibited chronic alcohol consumption by 70% (P < 0.001), an effect seen within the first 24 hours of hMSCs administration, and reduced relapse-like drinking by 80% (P < 0.001). In the relapse-like condition, control animals attain blood ethanol ('binge-like') levels >80 mg/dl. The single hMSC administration reduced relapse-like blood ethanol levels to 20 mg/dl. Chronic ethanol intake increased by 250% (P < 0.001) the levels of reactive oxygen species in hippocampus, which were markedly reduced by hMSC administration. Astrocyte glial acidic fibrillary protein immunoreactivity, a hallmark of neuroinflammation, was increased by 60-80% (P < 0.001) by chronic ethanol intake, an effect that was fully abolished by the administration of hMSCs. This study supports the neuroinflammation-chronic ethanol intake hypothesis and suggest that mesenchymal stem cell administration may be considered in the treatment of alcohol use disorders.

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Section: Discussionmentioning

confidence: 99%

“…; Costa et al . ). Some reports indicate that ROS lead to inactivation of IkB, the NFkB inhibitor, allowing the entrance of the NFkB p65 moiety into the nucleus, which activates the synthesis of pro‐inflammatory cytokines, including TNF‐α (Kastl et al .…”

mentioning

confidence: 97%

Activated mesenchymal stem cell administration inhibits chronic alcohol drinking and suppresses relapse‐like drinking in high‐alcohol drinker rats

Ezquer

Quintanilla²,

Morales³

et al. 2017

Addiction Biology

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show abstract

“…Ethanol has a direct toxic effect on the hepatocyte and could induce severe liver perturbations of cholesterol and triglycerides metabolism [ 26 ]. Ethanol is also a neurotoxic agent that induces degeneration and damage to the brain, and alcohol drinking could impair the blood-brain barrier [ 27 , 28 ]. Since Citrus limon (yellow), Averrhoa carambola , Pyrus spp., and Syzygium samarangense juices could lower the concentration of ethanol in blood, consuming them might be beneficial when drinking alcohol.…”

Section: Resultsmentioning

confidence: 99%

Effects of 20 Selected Fruits on Ethanol Metabolism: Potential Health Benefits and Harmful Impacts

Zhang

Wang

Zhou

et al. 2016

IJERPH

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The consumption of alcohol is often accompanied by other foods, such as fruits and vegetables. This study is aimed to investigate the effects of 20 selected fruits on ethanol metabolism to find out their potential health benefits and harmful impacts. The effects of the fruits on ethanol metabolism were characterized by the concentrations of ethanol and acetaldehyde in blood, as well as activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in liver of mice. Furthermore, potential health benefits and harmful impacts of the fruits were evaluated by biochemical parameters including aspartate transaminase (AST), alanine transferase (ALT), malondialdehyde, and superoxide dismutase. Generally, effects of these fruits on ethanol metabolism were very different. Some fruits (such as Citrus limon (yellow), Averrhoa carambola, Pyrus spp., and Syzygium samarangense) could decrease the concentration of ethanol in blood. In addition, several fruits (such as Cucumis melo) showed hepatoprotective effects by significantly decreasing AST or ALT level in blood, while some fruits (such as Averrhoa carambola) showed adverse effects. The results suggested that the consumption of alcohol should not be accompanied by some fruits, and several fruits could be developed as functional foods for the prevention and treatment of hangover and alcohol use disorder.

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Adolescent alcohol binge drinking and withdrawal: behavioural, brain GFAP-positive astrocytes and acute methamphetamine effects in adult female rats

Costa,

Everett,

Turner

et al. 2024

Psychopharmacology

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Rationale Alcopop beverages are generally the first alcoholic beverage that young females drink which contain high levels of sugar and alcohol. The over-consumption of these drinks may encourage alcohol co-administration with methamphetamine (METH) impacting on drinking behaviour and glial function. Aims The aims of this study were to evaluate the effect of adolescent binge alcohol exposure on consumption level, anxiety-like behaviour, cross-sensitization with METH and on astrocyte expression in reward related brain regions. Methods Adolescent female Sprague-Dawley rats had daily 1-hour oral alcohol consumption of alcopop (ALCP; with sucrose) or ethanol-only (ETOH; without sucrose), transitioned from 5 to 15% (v/v) ethanol content for 34 days. Water and sucrose groups act as controls. During alcohol withdrawal, rats were tested for anxiety on the elevated plus maze (EPM) and locomotor activity following saline or METH (1 mg/kg i.p) treatment. Brains were then collected to assess astrocyte immunofluorescence for glial fibrillary acidic protein (GFAP) in reward-related brain regions. Results Rats pretreated with 5% ALCP consumed significantly more volume and ethanol intake when compared to 5% EtOH rats. Both ALCP and EtOH groups had a higher preference ratio for 5% than 15% alcohol solutions and ALCP rats had greater ethanol intake at 15% than EtOH rats. Alcohol withdrawal showed no significant differences between groups on anxiety, METH cross-sensitization effects or GFAP intensity in the regions studied. Conclusions Overall, the addition of sucrose to alcoholic solutions encouraged female rats to consume larger volumes and greater ethanol intake compared to ethanol-only solutions, yet did not have long lasting effects on behaviour and astrocytes.

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Brain DNA damage and behavioral changes after repeated intermittent acute ethanol withdrawal by young rats

Abstract: The animal model of repeated intermittent ethanol access induced behavioral changes in rats, and this ethanol exposure model induced an increase in DNA single-strand breaks and ROS production in all brain areas. Our results suggest that these brain damages may influence future behaviors.

Cited by 11 publications

References 64 publications

Activated mesenchymal stem cell administration inhibits chronic alcohol drinking and suppresses relapse‐like drinking in high‐alcohol drinker rats

Activated mesenchymal stem cell administration inhibits chronic alcohol drinking and suppresses relapse‐like drinking in high‐alcohol drinker rats

Effects of 20 Selected Fruits on Ethanol Metabolism: Potential Health Benefits and Harmful Impacts

Adolescent alcohol binge drinking and withdrawal: behavioural, brain GFAP-positive astrocytes and acute methamphetamine effects in adult female rats

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